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Molecular detection of Potato yellow vein virus in the natural whitefly vector Trialeurodes vaporariorum, Westwood Rev. Protección Veg.
Barragan,Carlos Eduardo; Guzmán-Barney,Mónica.
Potato yellow vein virus (PYVV) expresses yellowing symptoms on plants and it is transmitted by the whitefly vector Trialeurodes vaporariorum Westwood. No molecular viral detection from the vector extracts has been reported to date. The objectives of this study were I) amplification of the PYVV major coat protein (CP) gene from viruliferous vector extracts by RT-PCR using specific primers, II) cloning and III) sequencing and sequences analysis. Some Colombian Solanum phureja (Jus et Buk) field potato plants expressing yellowing symptoms and confirmed PYVV positive by RT-PCR were used as virus donor plants for feeding whiteflies. Viruliferous vectors were obtained by depositing aviruliferous whiteflies on symptomatic leaves and kept in a muslin...
Tipo: Journal article Palavras-chave: PYVV; RNA; Whitefly; Detection; Vector.
Ano: 2014 URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1010-27522014000300002
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