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Registros recuperados: 26
Primeira ... 12 ... Última
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Acute and latent infection in mice with a virulent strain of Aujeszky’s disease virus BJM
Flatschart,Roberto B.; Resende,Maurício.
Acute and latent infections with the Brazilian LA031 strain of Aujeszky’s disease virus (ADV) were established in mice. Ultraviolet irradiated ADV administered subcutaneously was a successful way to establish latent infection. The presence of ADV was detected by PCR. Two sets of 22-mer primers were synthesized and used to amplify gG glycoprotein gene sequences in acute and latent infected trigeminal nerve ganglia. The specificity of the amplification was verified by dot-blot hybridization.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aujeszky’s disease virus; Polymerase chain reaction; Dot-blot hybridization.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000400013
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Allelic Analysis of Immunodominant Major Piroplasm Surface Protein Genes of Benign Theileria Parasites in Australian Cattle OAK
Kubota, Shuichi; Kakuda, Tsutomu; Sugimoto, Chihiro; Waltisbuhl, David; Onuma, Misao.
Palavras-chave: Allele; Benign Theileria; Piroplasm surface antigen; Polymerase chain reaction.
Ano: 1996 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/240
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Caracterizacao genetica do Trypanosoma vivax isolado no pantanal do Estado de Mato Grosso e o diagnostico diferencial da infeccao por Trypanosoma evansi pela reacao em cadeia da polimerase (PCR). Infoteca-e
MADRUGA, C.R.; MORZARIA, S.; MAJIWA, P.O..
1999
Tipo: Séries anteriores (INFOTECA-E) Palavras-chave: Trypanosoma vivax; Trypanosoma evansi; Diagnostico; Reacao da polimerase em cadeia; Pantanal; Pocone; Mato Grosso; Brasil; Diagnosis; Polymerase chain reaction; Brazil.
Ano: 1999 URL: http://www.infoteca.cnptia.embrapa.br/handle/doc/322100
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Clinical and Laboratory evaluation of measleslike rash in children and young adults BJM
Stewien,Klaus Eberhard; Lima,Lourdes Rehder de Andrade Vaz de; Botosso,Viviane Fongaro; Oliveira,Maria Isabel de; Fagundes,Simone N.; Nogueira,Meri B.; Ragazzi,Selma Lopes Betta; Costa,Maria Tereza Zuluni da; Ejzenberg,Bernardo; Durigon,Edison Luiz.
A clinical and laboratory evaluation of 11 children and young adults with measleslike rash was done during the measles outbreak in the Greater São Paulo Metropolitan area at the end of 1996 and spread over the country during 1997. Measles was laboratory confirmed in 07 patients by specific IgM detection in acute serum specimens using an IgM-capture EIA, by specific IgG seroconversion in serum pairs, and by reverse transcription PCR and virus isolation in peripheral blood lymphocytes. Clinical presentations were not always classic; one of the 07 cases had received measles vaccine and corresponded to modified clinical case of measles. The 4 remaining cases were negative for measles and were diagnosed as exanthem subitum (2 cases), scarlet fever and Kawasaki...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Measles; Clinical evaluation; Laboratory investigation; Polymerase chain reaction; Measles virus isolation; Enzyme immunoassay.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000400008
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Comparison of eight methods to isolate genomic DNA from Hancornia speciosa. Repositório Alice
ALMEIDA, V. M.; LUZ, G. A.; MARTINS, P. P.; GOMES, M. F. C.; COSTA, M. F.; LIMA, P. S. da C.; VALENTE, S. E. S..
Mangabeira (Hancornia speciosa) is a native fruit tree found mainly in the Cerrado biome and shows great economic potential due to its multiple uses; the fruits are used in agriculture, are important as a food resource, and can be consumed in natura or processed. Due to a reduction in the area of ecosystems where it occurs, mangabeira is threatened by genetic erosion in Brazil. The characterization of the genetic diversity of plants can provide the basis for strategies to protect and conserve endangered populations, like mangabeira. This study aimed to compare eight DNA extraction methods in mangabeira because the key to success is the use of a pure genomic DNA for the characterization of genetic diversity in molecular biology techniques. The quality and...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Isolamento do DNA; Mangabeira; Reação em cadeia da polimerase; DNA isolation; Polymerase chain reaction.
Ano: 2017 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1073486
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Deletion of DNA sequences of using a polymerase chain reaction based approach Electron. J. Biotechnol.
Pérez-Pinera,Pablo; Menéndez-González,Manuel; Vega,José Antonio.
We developed a simple, rapid and reliable method to delete DNA fragments in plasmids using a polymerase chain reaction based amplification of the circular DNA sequence that excludes the fragment to be deleted. The primers are designed to contain a non-complementary 5' sequence consisting of a restriction enzyme target sequence. Following PCR amplification, the plasmid is digested with Dpn I to eliminate the template DNA, with the chosen restriction enzyme, and ligated. The only limitation is the selection of the restriction enzyme target sequence that must not be present in the original plasmid. The method is straightforward in its execution and success relies on a meticulous primer design that permits us obtain 100% of transformants containing the desired...
Tipo: Journal article Palavras-chave: Deletion; Mutagenesis; Polymerase chain reaction; Plasmid.
Ano: 2006 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000500018
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Designing and validation of genus-specific primers for human gut flora study Electron. J. Biotechnol.
Rekha,Rani; Alam Rizvi,Moshahid; Jaishree,Paul.
The aim of this study, was to design and validate 16S rRNA targeted oligonucleotide genus specific primers for amplifying the predominant members of gut flora using polymerase chain reaction. Primers were validated against human faecal samples. Gut flora of a normal individual was compared with that of two diseased individuals. Our observations showed that the genera Lactobacillus, Bacteroides, Peptococcus, Bifidobacterium, and E. coli were invariably present in all studied subjects however, the absence of butyrate producing bacteria Ruminococcus and Peptostreptococcus were significant. Presence of the members of the genus, Campylobacter in both the diseased samples were also unusual.
Tipo: Journal article Palavras-chave: 16S rRNA based primers; Anaerobic bacteria; Diarrhoea patient; Polymerase chain reaction.
Ano: 2006 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000500005
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Detection of bovine Clostridium perfringens by polymerase chain reaction J. Venom. Anim. Toxins incl. Trop. Dis.
Piatti,R. M.; Ikuno,A. A.; Baldassi,L..
A polymerase chain reaction (PCR) assay to detect Clostridium perfringens alpha toxin gene (cpa) was used to identify eighty-nine C. perfringens strains obtained from bovine clinical material. The strains were biochemically characterized as C. perfringens. The isolated strains were cultured on plates containing brain heart infusion agar with 5% sheep blood under anaerobic conditions. DNA extraction was performed by boiling. The 324 bp amplification product of cpa was observed in all isolates. C. sordellii, C. botulinum, C. novyi, and C. septicum were also tested but did not produce any alpha toxin gene amplification. These findings suggest that PCR is a useful assay in identifying C. perfringens toxin types.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Clostridium perfringens; PCR; Alpha toxin; Polymerase chain reaction.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992004000200005
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Detection of different Brazilian strains of the bovine herpesvirus-1 (BHV-1) by polymerase chain reaction Arq. Bras. Med. Vet. Zootec.
Cândido,A.L.; Martins,A.S.; Barros,P.B.; Resende,M..
A técnica da reação em cadeia pela DNA polimerase (PCR) foi usada para a amplificação rápida de um fragmento de 456bp da região única curta (Us) do genoma do BHV-1. Iniciadores de 18pb do gene da ORF1 foram usados para a amplificação das amostras-padrão e brasileiras. Uma amplificação clássica não foi bem sucedida. A amplificação foi obtida quando se escolheu uma região com baixa concentração de GC no DNA do BHV-1 e através da desnaturação térmica (95° C para 5min) seguida de ciclos térmicos (94° C por 1min e 30seg; 52° C por 1min; 72° C por 1min e 30seg; e ainda por 35 ciclos de 94° C por 1min; 52° C por 1min; 72° C por 1min e 30seg; usando um tempo de extensão final de 72° C por 5min). A clivagem com Pst I confirmou a especificidade do fragmento da ORF 1...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Bovine herpesvirus 1; ORF-1; Polymerase chain reaction; Brazil.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09351999000300007
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Development of a polymerase chain reaction for the detection of abalone herpesvirus infection based on the DNA polymerase gene ArchiMer
Chen, M.h.; Kuo, S. T.; Renault, Tristan; Friedman, C. S.; Chang, P. H..
A 5781-base pair (bp) fragment of genomic DNA from the Taiwanese abalone herpesvirus was obtained and showed 99% (5767/5779) homology in the nucleotide sequence and 99% (1923/1926) in the amino acid sequence with the DNA polymerase gene of the abalone herpesvirus strain Victoria/AUS/2007. Homology of the amino acid sequence with the DNA polymerase of ostreid herpesvirus 1 was 30% (563/1856). In this study, a PCR-based procedure for detecting herpesvirus infection of abalone, Haliotis diversicolor supertexta, in Taiwan was developed. The method employed primer sets targeting the viral DNA polymerase gene, and was able to amplify DNA fragments of the expected size from infected samples. Primer sets of 40f and 146r were designed for amplification of an...
Tipo: Text Palavras-chave: Herpes virus; Abalone; Haliotis diversicolor supertexta; DNA polymerase; Polymerase chain reaction.
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00098/20952/19675.pdf
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Diagnosis of Trypanosoma evansi by the polymerase chain reaction (PCR) OAK
Donelson, J. E.; Artama, W. T..
Palavras-chave: Trypanosoma evansi; Diagnosis; Polymerase chain reaction.
Ano: 1998 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/300
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Diagnóstico molecular e análise filogenética de isolados brasileiros de Trypanosoma vivax baseado na reação da polimerase em cadeia - PCR. Infoteca-e
MADRUGA, C. R.; ARAÚJO, F. R. de; SOARES, C. O.; MELO, E. S. de P.; ALMEIDA, D. A.; ALMEIDA JÚNIOR, N. F. de; XAVIER, M. A. S.; OSÓRIO, A. L. A.; GÓES-CAVALCANTE, G.; RAMOS, C. A. do N..
2003
Tipo: Comunicado Técnico (INFOTECA-E) Palavras-chave: Trypanosoma vivax; Filogenia; Diagnóstico; Reação da polimerase em cadeia; Brasil; Phylogeny; Diagnosis; Polymerase chain reaction; Brazil.
Ano: 2003 URL: http://www.infoteca.cnptia.embrapa.br/handle/doc/325753
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Effect of single resistance genes and their pyramid on the diversity of Xanthomonas oryzae pv. oryzae population under field conditions as revealed by insertion sequence-polymerase chain reaction (IS PCR) International Rice Research Institute
Nguyen Dac Khoa.
xv, 105 leaves : ill. Thesis (M.S.) -- University of the Philippines at Los Baños
Tipo: Thesis Palavras-chave: Rice; Molecular genetics; Genetic resistance; Host-pathogen relationships; Xanthomonas oryzae pv. oryzae; Genetic variation; Transposons; Polymerase chain reaction.
Ano: 2005 URL: http://hdl.handle.net/123456789/358
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Epidemiological survey of Babesia gibsoni infection in dogs in eastern Japan OAK
Miyama, Takako; Sakata, Yoshimi; Shimada, Yojiro; Ogino, Shoji; Watanabe, Malaika; Itamoto, Kazuhito; Okuda, Masaru; Verdida, Rodolfo A.; Xuan, Xuenan; Nagasawa, Hideyuki; Inokuma, Hisashi.
To determine the distribution of Babesia gibsoni infection in dogs in the eastern part of Japan, an epidemiological survey of dogs suspected of having B. gibsoni infection was attempted using the polymerase chain reaction (PCR) and enzyme-linked immunosorbent assay (ELISA). Thirty-five of 115 such dogs (30.4%) were positive by PCR and/or ELISA. The 35 positive dogs consisted of 28 Tosa dogs, 4 American Pit Bull Terriers, and 3 mongrel dogs in Aomori, Fukushima, Ibaraki, Gunma, Chiba, Tokyo, Kanagawa, and Nagano Prefectures. The positive dogs had a significantly lower rate of tick exposure and a higher rate of bites by other dogs. Twenty-two of 35 B. gibsoni-positive dogs were infected with hemoplasma, and the rate of infection was significantly higher than...
Palavras-chave: Babesia gibsoni; Eastern Japan; Enzyme-linked immunosorbent assay; Hemoplasma; Polymerase chain reaction.
Ano: 2005 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/924
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Estabelecimento de PCR multiplex para detecção dos genes associados à virulência em isolados de Pasteurella multocida. Repositório Alice
REBELATTO, R.; OLIVEIRA FILHO, J. X. de; BELLAVER, F. A. V.; SERVELIN, E. C.; SILVA, G. B.; MORES, M. A. Z.; MORES, N.; KLEIN, C. S..
2013
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: PCR; Sanidade animal; Virologia; Suíno; Animal diseases; Virology; Swine; Polymerase chain reaction.
Ano: 2013 URL: http://www.alice.cnptia.embrapa.br/handle/doc/979704
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Estirpes de Bacillus thuringiensis efetivas contra insetos das ordens Lepidoptera, Coleoptera e Diptera. Repositório Alice
PRAÇA, L.B.; BATISTA, A.C.; MARTINS, E.S.; SIQUEIRA, C.B.; DIAS, D.G. de S.; GOMES, A.C.M.M.; FALCÃO, R.; MONNERAT, R.G..
O objetivo deste trabalho foi selecionar entre 300 estirpes de Bacillus thuringiensis as efetivas simultaneamente contra larvas de Spodoptera frugiperda J.E. Smith e Anticarsia gemmatalis Hübner (Lepidoptera: Noctuidae), Anthonomus grandis Boheman (Coleoptera: Curculionidae), Aedes aegypti Linnaeus e Culex quinquefasciatus Say (Diptera: Culicidae). Foram selecionadas duas estirpes de B. thuringiensis, denominadas S234 e S997, que apresentaram atividade contra as três ordens de insetos. As estirpes foram caracterizadas por métodos morfológicos, bioquímicos e moleculares. As mesmas apresentaram duas proteínas principais de 130 e 65 kDa, produtos de reação em cadeia da polimerase de tamanho esperado para a detecção dos genes cry1Aa, cry1Ab, cry1Ac, cry1B e...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Larva; Controle biológico; Proteína; Reação em cadeia da polimerase; Combate às pragas; Biopesticida; Larvae; Biological control; Protein; Polymerase chain reaction; Pest control; Biopesticides.
Ano: 2004 URL: http://www.alice.cnptia.embrapa.br/handle/doc/108616
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Experimental infection of dogs with Babesia microti OAK
Ohmori, T.; Uetsuka, K.; Nunoya, T.
Palavras-chave: Babesia microti; Dog; Experimental infection; Polymerase chain reaction; Indirect immunofluorescence test.
Ano: 2011 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3132
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Molecular diagnosis optimization of virus, bacteria and fungi in sugarcane. Repositório Alice
SAWAZAKI, H. E.; SA, L. A. N. de; GONÇALVES, C. R. N. C. B.; VEIGA, R. F. A.; COLOMBO, C. A..
Abstract: Aiming at optimizing the diagnosis of major sugarcane diseases by PCR, primers were developed (scald, orange rust) and amplified fragments according to the literature were used as positive control, such as those caused by: 1 - Bacteria, (ratoon stunting and leaf scald) 2 - Viruses, (yellow leaf, mosaic, mosaic streak and fijivirus) 3-Fungus, (smut, orange rust and curvularia). For diseases with long latency period (leaf scald, ratoon stunting, yellow leaf, mosaic, fijivirus, smut and orange rust), primers were designed for real time PCR. For testing, infected leaves, fungal colonies, amplified DNA fragment or 40 seedlings were used. Real time PCR analyses have enabled the detection of sugarcane samples with highest dilution of DNA. The pair of...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Leaf scald; PCR; Cana de açúcar; Doença de planta; Diagnóstico; Bactéria; Vírus; Fungo; Escaldadura; Ferrugem alaranjada; Plant diseases and disorders; Sugarcane; Ratoon stunting disease; Scald diseases; Sugarcane yellow leaf virus; Sugarcane streak virus; Fijivirus; Smut diseases; Rust diseases; Curvularia; Polymerase chain reaction.
Ano: 2013 URL: http://www.alice.cnptia.embrapa.br/handle/doc/963146
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Monitoring Trypanosoma cruzi infection in triatomines using PCR in Mato Grosso do Sul, Brazil Repositório Alice
COMINETTI, M. C.; ALMEIDA, R. F. C. de; GONÇALVES, G. M. do A.; ANDREOTTI, R..
2013
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Epidemiology; Triatomine infection monitoring; Polymerase chain reaction; Microscopy examination; Trypanosoma cruzi.
Ano: 2013 URL: http://www.alice.cnptia.embrapa.br/handle/doc/970527
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Multiplex PCR sets of novel microsatellite loci for the great scallop Pecten maximus and their application in parentage assignment ArchiMer
Morvezen, Romain; Cornette, Florence; Charrier, Gregory; Guinand, Bruno; Lapegue, Sylvie; Boudry, Pierre; Laroche, Jean.
We report the isolation, development and multiplex optimisation of 12 new microsatellite loci for the great scallop, Pecten maximus. Diversity was moderate to high, with number of alleles ranging from 4 to 20 and observed heterozygosity between 0.28 and 0.88. Progeny produced in a commercial hatchery was used to test locus power for parentage assignment. The percentage of offspring that was unambiguously assigned to a unique pair of parents was 97% (software package CERVUS-COLONY). Parentage assignment revealed that 22% of the studied progeny resulted from unplanned crosses. Effective population size of the study progeny was also estimated. Our study illustrates the power of microsatellites for the genetic monitoring of hatchery-produced great scallops.
Tipo: Text Palavras-chave: Microsatellites; Polymerase chain reaction; Parentage; Assignment; Bivalve mollusc.
Ano: 2013 URL: http://archimer.ifremer.fr/doc/00137/24822/22907.pdf
Registros recuperados: 26
Primeira ... 12 ... Última
 

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