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Genotypic profile of Pantanal creole sheep regarding susceptibility or resistance to scrapie. Repositório Alice
GONÇALVES, A. N. D.; SOARES, C. O.; SANCHES, S. C.; REIS, F. A.; ROSINHA, G. M. S..
The objective of this work was to determine the genotypic profile specific to scrapie in codons 136, 154, and 171 of the PRNP gene of the Pantanal creole sheep. Genomic DNA was extracted from blood samples collected from 66 sheep, and the regions of interest on the DNA strand were amplified by PCR. Five haplotypes were identified: ARR, alanine, arginine, arginine; ARQ, alanine, arginine, glutamine; AHQ, alanine, histidine, glutamine; ARH, alanine, arginine, histidine; and VRQ, valine, arginine, glutamine. The most common genotypes were ARQ/ARQ (27%) and ARR/ARQ (24%). The genotypic profile of the Pantanal creole sheep shows low to moderate susceptibility.
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Encefalopatia espongiforme transmissivel; Gene PRNP; Genotipagem; Polimorfismo de nucleotídeo único; Single nucleotide polymorphisms; Transmissible spongiform encephalopathies; Genotyping; Prion diseases.
Ano: 2016 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1050554
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Prions: the danger of biochemical weapons Ciênc. Tecnol. Aliment.
Xavier,Eric Almeida.
The knowledge of biotechnology increases the risk of using biochemical weapons for mass destruction. Prions are unprecedented infectious pathogens that cause a group of fatal neurodegenerative diseases by a novel mechanism. They are transmissible particles that are devoid of nucleic acid. Due to their singular characteristics, Prions emerge as potential danger since they can be used in the development of such weapons. Prions cause fatal infectious diseases, and to date there is no therapeutic or prophylactic approach against these diseases. Furthermore, Prions are resistant to food-preparation treatments such as high heat and can find their way from the digestive system into the nervous system; recombinant Prions are infectious either bound to soil...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Prions; Biochemical weapons; Prion diseases; Prions danger to the environment; Prions risk alert.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612014000300001
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Traceability of animal protein byproducts in ruminants by multivariate analysis of isotope ratio mass spectrometry to prevent transmission of prion diseases J. Venom. Anim. Toxins incl. Trop. Dis.
Ferreira Jr.,Rui Seabra; Silva,Daniela Alessandra Fossato da; Biscola,Natália Perussi; Sartori,Maria Márcia Pereira; Denadai,Juliana Célia; Jorge,André Mendes; Santos,Lucilene Delazari dos; Barraviera,Benedito.
Abstract Background: Ruminant feed containing animal byproduct proteins (ABPs) is prohibited in many countries due to its risk of transmitting prion diseases (PD). In most cases the entire herd is sacrificed, which causes great harm to the producer countries by preventing their exportation of ruminant derived-products. Methods: We used stable isotope ratio mass spectrometry (IRMS) of carbon (13C/12C) and nitrogen (15N/14N) to trace the animal protein in the blood of 15 buffaloes (Bubalus bubalis) divided into three experimental groups: 1 - received only vegetable protein (VP) during 117 days; 2 - received animal and vegetable protein (AVP); and 3 - received animal and vegetable protein with animal protein subsequently removed (AVPR). Groups 2 and 3...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Prion diseases; Monitoring; Bovine spongiform; Encephalopathy; Stable isotopes.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992019000100312
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