Sabiia Seb
PortuguêsEspañolEnglish
Embrapa
        Busca avançada

Botão Atualizar


Botão Atualizar

Ordenar por: RelevânciaAutorTítuloAnoImprime registros no formato resumido
Registros recuperados: 26
Primeira ... 12 ... Última
Imagem não selecionada

Imprime registro no formato completo
Assessment of primers designed from the small ribosomal subunit RNA for specific discrimination between Babesia bigemina and Babesia bovis by PCR UnB - FAB
Linhares, Guido Fontgalland Coelho; Santana, Ângela Patrícia; Lauerman, Lloyd Herman; Madruga, Cláudio Roberto.
ABSTRACT
Tipo: Article Palavras-chave: Babesia bovis; Babesia bigemina; RNA.
Ano: 2002 URL: http://hdl.handle.net/10482/7855
Imagem não selecionada

Imprime registro no formato completo
Campomanesia adamantium (Cambess.) O. Berg seed desiccation: influence on vigor and nucleic acids Anais da ABC (AABC)
DRESCH,DAIANE M.; MASETTO,TATHIANA E.; SCALON,SILVANA P.Q..
The aim of this study was to evaluate the sensitivity of Campomanesia adamantium seeds to desiccation by drying in activated silica gel (fast) and under laboratory conditions (slow). To assess the sensitivity of the seeds to desiccation, we used drying with silica gel and drying under laboratory conditions (25 °C), in order to obtain seeds with moisture content of 45, 35, 30, 25, 20, 15, 10 and 5%. The physiological potential of the seeds after desiccation was evaluated by measuring primary root protrusion, percentage of normal seedlings, germination seed index, seedling length, total seedling dry mass, electrical conductivity and DNA and RNA integrities. The C. adamantium seeds were sensitive to desiccation and to a reduction in moisture content to 21.1%...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cerrado; DNA; Drying; RNA; Viability.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000502217
Imagem não selecionada

Imprime registro no formato completo
Comparison of commercially-available RNA extraction methods for effective bacterial RNA isolation from milk spiked samples Electron. J. Biotechnol.
García-Nogales,Paula; Serrano,Alicia; Secchi,Sonia; Gutiérrez,Serafín; Arís,Anna.
Nucleic-acid based methods for bacterial identification are extremely useful in diagnostic applications due to their specificity and sensitivity. However, they require an optimal purification of the target molecules. As part of the development of a new diagnostic method for the detection of bacterial RNA in cow milk, we have compared four commercially available RNA extraction kits for the isolation of bacterial RNA from spiked UHT milk samples. The kits were compared in terms of extraction efficiency and RNA purity using two bacterial species, the Gram negative Escherichia coli and the Gram positive Staphylococcus aureus. Two kits are based in silica-matrix extraction, and the other two in the guanidinium thiocyanate-phenol-chloroform extraction. In our...
Tipo: Journal article Palavras-chave: Bacteria; Milk; Purification; RNA.
Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000500019
Imagem não selecionada

Imprime registro no formato completo
Control of gene expression in Trypanosomatidae BJMBR
Teixeira,S.M.R..
The study of mechanisms which control gene expression in trypanosomatids has developed at an increasing rate since 1989 when the first successful DNA transfection experiments were reported. Using primarily Trypanosoma brucei as a model, several groups have begun to elucidate the basic control mechanisms and to define the cellular factors involved in mRNA transcription, processing and translation in these parasites. This review focuses on the most recent studies regarding a subset of genes that are expressed differentially during the life cycle of three groups of parasites. In addition to T. brucei, I will address studies on gene regulation in a few species of Leishmania and the results obtained by a much more limited group of laboratories studying gene...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Trypanosoma; Leishmania; Gene expression; RNA; Antigenic variation; VSG.
Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001200001
Imagem não selecionada

Imprime registro no formato completo
coordinated research on groundnut of Rosette virus disease Open Agri
D. McDonald.
Palavras-chave: Groundnuts; Viruses; RNA; Grafting; ELISA; Genotypes; Backcrossing; Breeds (animals); Resistance varieties; Scions.
Ano: 1987 URL: http://agropedia.iitk.ac.in/openaccess/?q=node/3144
Imagem não selecionada

Imprime registro no formato completo
Coordinated Research on Groundnut Rosette Virus Disease Open Agri
Palavras-chave: Groundnuts; Viruses; RNA; Grafting; ELISA; Genotypes; Backcrossing; Breeds (animals); Resistance varieties; Scions.
Ano: 1988 URL: http://agropedia.iitk.ac.in/openaccess/?q=node/4054
Imagem não selecionada

Imprime registro no formato completo
De novo transcriptome assembly, functional annotation and differential gene expression analysis of juvenile and adult E. fetida, a model oligochaete used in ecotoxicological studies Biol. Res.
Thunders,Michelle; Cavanagh,Jo; Li,Yinsheng.
Abstract Background Earthworms are sensitive to toxic chemicals present in the soil and so are useful indicator organisms for soil health. Eisenia fetida are commonly used in ecotoxicological studies; therefore the assembly of a baseline transcriptome is important for subsequent analyses exploring the impact of toxin exposure on genome wide gene expression. Results This paper reports on the de novo transcriptome assembly of E. fetida using Trinity, a freely available software tool. Trinotate was used to carry out functional annotation of the Trinity generated transcriptome file and the transdecoder generated peptide sequence file along with BLASTX, BLASTP and HMMER searches and were loaded into a Sqlite3 database. To identify differentially expressed...
Tipo: Journal article Palavras-chave: Earthworm; Trinity; Transcriptome; E. fetida; Ecotoxicology; RNA; Sequence; Gene expression.
Ano: 2017 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100401
Imagem não selecionada

Imprime registro no formato completo
Development of genomic probes for detection of hepatitis A virus and enteroviruses in shellfish ArchiMer
Apaire, M; Leguyader, F; Besse, B; Billaudel, S.
The development of molecular biology techniques has modified research procedures for certain viruses, particularly hepatitis A virus (HAV) and enteroviruses. Accordingly, we designed cDNA and RNA genomic probes to detect viruses in shellfish in the environment. Better sensitivity was noted with RNA than cDNA probes: only 17/83 shellfish samples were positive with HAV and enterovirus DNA probes, where as 42/83 were positive with HAV and enterovirus RNA probes.
Tipo: Text Palavras-chave: Mollusca; Enterovirus; Hepatitis A virus; Picornaviridae; RNA probes; DNA probes; Seafood; Biology; Sensors; DNA; RNA; Viruses; Detection; Marine molluscs; Shellfish.
Ano: 1992 URL: http://archimer.ifremer.fr/doc/1992/acte-1613.pdf
Imagem não selecionada

Imprime registro no formato completo
Differential gene expression profiles in peripheral blood in Northeast Chinese Han people with acute myocardial infarction Genet. Mol. Biol.
Fan,Lin; Meng,Heyu; Guo,Xudong; Li,Xiangdong; Meng,Fanbo.
Abstract This study aimed to use gene chips to investigate differential gene expression profiles in the occurrence and development of acute myocardial infarction (AMI). The study included 12 AMI patients and 12 healthy individuals. Total mRNA of peripheral bloodwas extracted and reversed-transcribed to cDNA for microarray analysis. After establishing two pools with three subjects each (3 AMI patients and 3 healthy individuals), the remaining samples were used for RT-qPCR to confirm the microarray data. From the microarray results, seven genes were randomly selected for RT-qPCR. RT-qPCR results were analyzed by the 2-ΔΔCt method. Microarray analysis showed that 228 genes were up- regulated and 271 were down-regulated (p ≤ 0.05, |logFC| > 1). Gene...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Acute myocardial infarction; RNA; Differential expression.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000100059
Imagem não selecionada

Imprime registro no formato completo
Effects of cottonseed meal supplemented with lysine and enzyme (Hydroenzyme XP) on egg quality and performance of laying hens R. Bras. Zootec.
Saki,Ali Asghar; Pournia,Khashayar; Tabatabaie,Mohamad Mehdi; Zamani,Pouya; Haghighat,Mojtaba; Salary,Jalal.
This study was conducted to evaluate the effect of cottonseed meal supplemented with lysine and enzyme on laying hens performance and egg quality. Eighty Hy-Line W-36 white Leghorns were allotted for 12 weeks in a 2 × 2 factorial experiment in a completely randomized design, including four treatments and five replications with four birds in each. Treatments included: basal diet + 1% lysine + 0% enzyme; basal diet + 1% lysine + 0.025% enzyme; basal diet + 2% lysine + 0% enzyme; and basal diet + 2% lysine + 0.025% enzyme. Protein content in the magnum tissue was not significantly affected by different levels of lysine and enzyme, although magnum Protein:RNA ratio increased with 2% of lysine as compared with 1%. Moreover, jejunum DNA's concentration was not...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cottonseed meal; DNA; Performance; Protein synthesis; RNA.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982012001000013
Imagem não selecionada

Imprime registro no formato completo
Engenharia genética: conceitos básicos, ferramentas e aplicações. Infoteca-e
CORDEIRO, M. C. R..
ABSTRACT: Basic concepts and methodologies concerning genetic engineering are presented in this review and is directed to people or professionales not familiarized to it. Moreover, it can be a document to clarity what is genetic engineering, the importance of its contributions to modern agriculture, medicine or livestock research and to support discussions on genetic research ethics. Covers information on structure of nucleic acids, types, functions and main processes such us DNA duplication, transcription and traduction. Important methodologies are covered about DNA/RNA extraction, electrophoresis, PCR, nucleic acid libraries production, cloning, sequencing, gene characterization in connection to main historical events of genetic engineering. It...
Tipo: Documentos (INFOTECA-E) Palavras-chave: Ácido Nucléico; Biologia Molecular; Biotecnologia; Clonagem; DNA; Engenharia Genética; Gene; Marcador Genético; RNA; Organismo Transgênico..
Ano: 2003 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/568132
Imagem não selecionada

Imprime registro no formato completo
Gene transcription in Lactarius quietus-Quercus petraea ectomycorrhizas from a forest soil Inra
Courty, P.E.; Poletto, M; Duchaussoy, F.; Buee, M.; Garbaye, J.; Martin, F..
Extracting fungal mRNA from ectomycorrhizas (ECMs) and forest soil samples for monitoring in situ metabolic activities is a significant challenge when studying the role of ECMs in biogeochemical cycles. A robust, simple, rapid, and effective method was developed for extracting RNA from rhizospheric soil and ECMs by adapting previous grinding and lysis methods. The quality and yield of the extracted RNA were sufficient to be used for reverse transcription. RNA extracted from ECMs of Lactarius quietus in a 100-year-old oak stand was used to construct a cDNA library and sequence expressed sequence tags. The transcripts of many genes involved in primary metabolism and in the degradation of organic matter were found. The transcription levels of four...
Tipo: Journal Article Palavras-chave: ECTOMYCORHIZE AMINO ACIDS; ECTOMYCORRHIZAS; FOREST SOILS; GENE EXPRESSION; GLUTAMATE-AMMONIA LIGASE; METABOLISM; MYCORRHIZAL FUNGI; N-ACETYL-BETA-GLUCOSAMINIDASE; RHIZOSPHERE; RNA; SOIL TYPES; BETA-N-ACETYLGLUCOSAMINIDASE; DNA TRANSCRIPTION; GLUTAMINE SYNTHETASE; RIBONUCLEIC ACID; TYROSINASE.
Ano: 2008 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2010e4344c33&uri=/notices/prodinra1/2010/11/
Imagem não selecionada

Imprime registro no formato completo
Groundnut Virus Diseases in Africa Open Agri
International Crops Research Institute for the Semi-Arid Tropics (ICRISAT).
Palavras-chave: Groundnuts; Viruses; Genotypes; RNA; Quarantine; Breeds (animals); Genetic markers; Genes; Recessive genes; Resistance varieties.
Ano: 1991 URL: http://agropedia.iitk.ac.in/openaccess/?q=node/4093
Imagem não selecionada

Imprime registro no formato completo
LA DUALIDAD ANÁLOGO DIGITAL DE LA INFORMACIÓN SE EJEMPLIFICA EN EL ESTUDIO DE LAS MÓLECULAS DE RNA Acta biol.Colomb.
ANDRADE,EUGENIO.
Las investigaciones sobre las moléculas de RNA, han contribuido a precisar y formalizar la noción física de información considerada tanto en su aspecto analógico como digital. El dogma central de la biología molecular, restringe la noción de información al aspecto digital de la misma, y por tanto al copiado fiel de una cadena de símbolos en los procesos de replicación (DNA-DNA) y transcripción (DNA-RNA), e igualmente en la traducción a proteínas mediante el código genético. Esta visión genera la paradoja sobre qué surgió primero si DNA o proteínas, la cual se resuelve en parte recurriendo a la hipótesis según la cual existió un mundo prebiótico RNA, anterior a las proteínas codificadas genéticamente y al DNA. Esta hipótesis tiene soporte empírico sólido...
Tipo: Info:eu-repo/semantics/article Palavras-chave: RNA; DNA; Proteínas; Información digital; Información analógica; Dogma central; Mundo RNA.
Ano: 2011 URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2011000300002
Imagem não selecionada

Imprime registro no formato completo
Liaison de l'oestradiol 17 beta dans les hepatocytes de truite Arc-en-ciel et induction de la vitellogenine et de son ARNm specifique ArchiMer
Maitre, J; Mercier, L; Dolo, L; Valotaire, Y.
In the liver of rainbow trout (Salmo gairdnerii ), vitellogenin (Vg) synthesis is influenced by oestradiol (E sub(2)) which is believed to act through the classical mechanism of steroid hormone action. After binding of the hormone to a soluble specific receptor protein, the oestradiol-receptor complex is translocated to the nucleus, where it interacts with DNA and modulates expression of Vg genes, leading to increased synthesis of specific mRNA and Vg. The authors show here: (i) the presence of specific oestrogen receptors in the cytosol of the male trout liver. (ii) The male liver, offering, an ideal experimental control of "zero" background, the authors followed - in the liver of male trouts - the kinetics of induction of Vg mRNA by hybridization with Vg...
Tipo: Text Palavras-chave: Salmonidae; Pisces; Proteins; Liver; RNA; Steroids; Biosynthesis; Receptors; Sex hormones; Sexual reproduction.
Ano: 1983 URL: http://archimer.ifremer.fr/doc/1983/acte-1230.pdf
Imagem não selecionada

Imprime registro no formato completo
Molecular detection of Potato yellow vein virus in the natural whitefly vector Trialeurodes vaporariorum, Westwood Rev. Protección Veg.
Barragan,Carlos Eduardo; Guzmán-Barney,Mónica.
Potato yellow vein virus (PYVV) expresses yellowing symptoms on plants and it is transmitted by the whitefly vector Trialeurodes vaporariorum Westwood. No molecular viral detection from the vector extracts has been reported to date. The objectives of this study were I) amplification of the PYVV major coat protein (CP) gene from viruliferous vector extracts by RT-PCR using specific primers, II) cloning and III) sequencing and sequences analysis. Some Colombian Solanum phureja (Jus et Buk) field potato plants expressing yellowing symptoms and confirmed PYVV positive by RT-PCR were used as virus donor plants for feeding whiteflies. Viruliferous vectors were obtained by depositing aviruliferous whiteflies on symptomatic leaves and kept in a muslin...
Tipo: Journal article Palavras-chave: PYVV; RNA; Whitefly; Detection; Vector.
Ano: 2014 URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1010-27522014000300002
Imagem não selecionada

Imprime registro no formato completo
Monitoring the regulation of gene expression in a growing organ using a fluid mechanics formalism Inra
Merret, R.; Moulia, B.; Hummel, I.; Cohen, D.; Dreyer, E.; Bogeat, M.B..
Background: Technological advances have enabled the accurate quantification of gene expression, even within single cell types. While transcriptome analyses are routinely performed, most experimental designs only provide snapshots of gene expression. Molecular mechanisms underlying cell fate or positional signalling have been revealed through these discontinuous datasets. However, in developing multicellular structures, temporal and spatial cues, known to directly influence transcriptional networks, get entangled as the cells are displaced and expand. Access to an unbiased view of the spatiotemporal regulation of gene expression occurring duringdevelopment requires a specific framework that properly quantifies the rate of change of a property in a moving...
Tipo: Journal Article Palavras-chave: POPULUS DELTOIDES × NIGRA CV 'SOLIGO'); GENE EXPRESSION; MONITORING; REAL-TIME POLYMERASE CHAIN REACTION; KINEMATICS; GROWTH REGULATION; VELOCITY; RNA.
Ano: 2010 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2011c2f24ced&uri=/notices/prodinra1/2011/01/
Imagem não selecionada

Imprime registro no formato completo
Response of Paenibacillus polymyxa to iron: alternations in cellular chemical composition and the production of fusaricidin type antimicrobial compounds BABT
Raza,Waseem; Hongsheng,Wu; Qirong,Shen.
In this work, growth, cellular chemical composition and production of fusaricidin type antimicrobial compounds by P. polymyxa SQR-21 were compared in tryptone broth supplemented with four concentrations of iron (25, 50, 100 and 200 µM). The data revealed that the growth of P. polymyxa SQR-21 was increased by 3-8% with the increase in concentration of ferric ion (Fe3+). The production of fusaricidin type compounds was increased by 33-49% only up to 50 µM Fe3+ and the highest level of Fe3+ was inhibitory. Increase in the liquid culture Fe3+concentration increased the intracellular protein (2%), intracellular carbohydrate (14%), extracellular protein (7%) and polysaccharide contents (18%) while the intracellular lipid contents were increased (11%) only up to...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chemical composition; Fusaricidin; Iron; Paenibacillus polymyxa; RNA.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000500019
Imagem não selecionada

Imprime registro no formato completo
Review: Genetics and genomics in equine exercise physiology: an overview of the new applications of molecular biology as positive and negative markers of performance and health Inra
Barrey, E..
Equine breeding selection has been developed by applying quantitative genetic methods for calculating the heritability of the complex traits such as performance in racing or sport competitions. With the great development of biotechnologies, equine molecular genetics has come of age. The recent sequencing of the equine genome by an international consortium was a major advance that will impact equine genomics in the near future. With the rapid progress in equine genetics, new applications in early performance evaluation and the detection of disease markers become available. Many new biomolecular tools will change management of horse selection, disease diagnosis and treatment. The purpose of this review is to present new developments in equine genetics and...
Tipo: Journal Article Palavras-chave: Horse; Gene; Transcript; RNA; DNA; Microarray; SNP; Exercise; Muscle.
Ano: 2010 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20101f0f686a&uri=/notices/prodinra1/2011/02/
Imagem não selecionada

Imprime registro no formato completo
The effect of nucleic acids on growth, ureagenesis and nitrogen excretion of gilthead sea bream Sparus aurata juveniles ArchiMer
Oliva Teles, A; Guedes, M; Vachot, Christianne; Kaushik, Sadasivam.
The aim of this trial was to evaluate the effect of dietary nucleic acids on ureagenesis and nitrogen balance in gilthead sea bream juveniles. For that purpose, 5 isonitrogenous (7.25% N) diets were formulated based on fish meal as protein source (FM-control diet) and to partially replace the fish meal nitrogen with two levels of nucleic acids (diets RNA1 and RNA2) or brewers yeast (diets BY1 and BY2). Each diet was fed for 10 weeks, to apparent visual satiety, to triplicate groups of 50 fish each with an average body weight of 12.7 g. Inclusion of nucleic acid N either as brewers yeast or RNA led to significant improvement of feed intake and growth, except for fish fed diet RNA2. There were no differences in final weight between groups fed the yeast-...
Tipo: Text Palavras-chave: Sea bream; N accretion; Ureagenesis; Brewers yeast; RNA.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/publication-6681.pdf
Registros recuperados: 26
Primeira ... 12 ... Última
 

Empresa Brasileira de Pesquisa Agropecuária - Embrapa
Todos os direitos reservados, conforme Lei n° 9.610
Política de Privacidade
Área restrita

Embrapa
Parque Estação Biológica - PqEB s/n°
Brasília, DF - Brasil - CEP 70770-901
Fone: (61) 3448-4433 - Fax: (61) 3448-4890 / 3448-4891 SAC: https://www.embrapa.br/fale-conosco

Valid HTML 4.01 Transitional