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Development and assessment of a latex agglutination test based on recombinant MSP5 to detect antibodies against Anaplasma marginale in cattle BJM
Ramos,Carlos A.N.; Araújo,Flábio R.; Santos,Rafaelle C.; Melo,Elaine S.P.; Sousa,Letícia C.; Vidal,Carlos E.S.; Guerra,Neurisvan R.; Ramos,Rafael A.N..
The recombinant protein MSP5 has been established as an important antigen for serological diagnosis of Anaplasma marginale by enzyme-linked immunosorbent assay (ELISA). However, due to the high cost of specialized equipment, this technique is not accessible to all laboratories, especially in developing countries in areas where the disease is endemic. The present study describes the standardization of a latex agglutination test (LAT) to detect antibodies against A. marginale based on recombinant MSP5. Compared with indirect enzyme-linked immunosorbent assay (iELISA), the relative sensitivity and specificity of the LAT were 95.21% and 91.86% respectively, with an almost perfect agreement between tests (kappa index = 0.863). These results can be considered...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Latex agglutination; Rapid test; Anaplasma marginale; MSP5.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000100026
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Evaluation of rapid tests for human immunodeficiency virus as a tool to detect recent seroconversion BJID
Girardi,Samira Barboza; Barreto,Angela Maria Egydio de Carvalho; Barreto,Claudia Cortese; Proietti,Anna Barbara; Carvalho,Silvia Maia Farias de; Loureiro,Paula; Sabino,Ester Cerdeira.
The identification of recent HIV infection is important for epidemiological studies and to monitor the epidemic. The objective of this study was to evaluate two rapid tests that are easily available to the Brazilian scientific community for using as markers of recent HIV infection. The Rapid Test - HIV-1/2 Bio-Manguinhos (Bio-Manguinhos/Fiocruz, Brazil) and the Rapid Check HIV 1&2 (NDI-UFES, Center for Infectious Diseases, Universidade Federal do Espírito Santo) were tested, using 489 samples with HIV positive serology, from blood donors, previously classified as recent or long-term infection by serological testing algorithm for recent HIV seroconversion (STARHS) or LS-HIV Vitros assay methods. The samples were diluted prior to testing (1:50 and 1:100...
Tipo: Info:eu-repo/semantics/article Palavras-chave: HIV-1 incidence; Detuned assay; Surveillance; Rapid test.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702012000500009
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