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Double-stranded RNA high-throughput sequencing reveals a new Cytorhabdovirus in a bean golden mosaic virus-resistant common bean transgenic line. Repositório Alice
ALVES-FREITAS, D. M. T.; PINHEIRO-LIMA, B.; FARIA, J. C.; LACORTE, C.; RIBEIRO, S. G.; MELO, F. L..
Using double-strand RNA (dsRNA) high-throughput sequencing, we identified five RNA viruses in a bean golden mosaic virus (BGMV)-resistant common bean transgenic line with symptoms of viral infection. Four of the identified viruses had already been described as infecting common bean (cowpea mild mottle virus, bean rugose mosaic virus, Phaseolus vulgaris alphaendornavirus 1, and Phaseolus vulgaris alphaendornavirus 2) and one is a putative new plant rhabdovirus (genus Cytorhabdovirus), tentatively named bean-associated cytorhabdovirus (BaCV). The BaCV genome presented all five open reading frames (ORFs) found in most rhabdoviruses: nucleoprotein (N) (ORF1) (451 amino acids, aa), phosphoprotein (P) (ORF2) (445 aa), matrix (M) (ORF4) (287 aa), glycoprotein (G)...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Rhabdovirus; Feijão; Phaseolus Vulgaris; Organismo Transgênico; Vírus; Mosaico Dourado; Beans; Cytorhabdovirus; DsRNA viruses; Transgenic plants; Bean golden mosaic virus.
Ano: 2019 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1104582
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First isolation of hirame rhabdovirus from freshwater fish in Europe ArchiMer
Borzym, E.; Matras, M.; Maj-paluch, J.; Baud, M.; De Boisseson, C.; Talbi, Chiraz; Olesen, N. J.; Bigarre, L..
A rhabdovirus was isolated in cell culture inoculated with tissue material from diseased grayling, Thymallus thymallus (L.), originating from a fish farm affected by a mortality episode in Poland. Diagnostics tests showed that the virus was not related to novirhabdoviruses known in Europe, nor to vesiculovirus-like species, except perch rhabdovirus (PRhV) with which it shared moderate serological relations. However, RT-PCR with PRhV probes gave negative results. To identify the virus, a random-priming sequence-independent single primer amplification was adopted. Surprisingly, two of the obtained sequences exhibited a high identity (>99%) with hirame rhabdovirus (HIRRV), a novirhabdovirus usually found in fish in marine Asiatic countries, for instance...
Tipo: Text Palavras-chave: Outbreak; Brown trout; Rhabdovirus; Sequence-independent single primer amplification; Molecular tracing; Grayling.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00197/30852/32727.pdf
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