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| Registros recuperados: 25 | |
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| Roustan, J.L.; Rascon Chu, A.; Moulin, G.; Bigey, F.. |
| A gene encoding an extracellular lipase (CaLIP4) from Candida albicans was successfully expressed in Saccharomyces cerevisiae after mutagenesis of its unusual CUG serine codon into a universal one. The ability of this lipase, which shares 60% sequence homology with the lipase/acyltransferase from Candida parapsilosis, to synthesise esters was investigated. CaLIP4 behaved as a true lipase, displaying activity towards insoluble triglycerides and having no activity in the presence of short-chain fatty acid (FA) esters and phosphatidylcholine. Methyl, ethyl and propyl esters were efficiently used. The lipase exhibited highest selectivity for unsaturated FA. With saturated FAs, C14-C16 acyl chains were preferred. In a biphasic aqueous/lipid system, CaLIP4... |
| Tipo: Journal Article |
Palavras-chave: CANDIDA ALBICANS; CANDIDA PARAPSILOSIS; SACCHAROMYCES CEREVISIAE; ALCOHOLYSIS ACTIVITY; LIPASE/ACYLTRANSFERASE; KINETIC CONTROL. |
| Ano: 2005 |
URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20084762bbfc&uri=/notices/prodinra1/2010/09/ |
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| Cheraiti, N.; Sauvage, F.X.; Salmon, J.M.. |
| During experiments to determine the effects of exogenously added acetaldehyde on pure cultures of various yeast strains, we discovered that an early acetaldehyde perfusion during the growth phase allowed several yeasts to partially overcome the phenotypic effects of zinc depletion during alcoholic fermentation. We, therefore, performed genome-wide expression and proteomic analysis on an industrial Saccharomyces cerevisiae yeast strain (VL1) growing in zinc-replete or zinc-depleted conditions in the presence of perfused acetaldehyde to identify molecular markers of this effect. Zinc depletion severely affects ethanol production and therefore nicotinamide adenine dinucleotide (NAD) regeneration, although we observed partial compensation by the upregulation... |
| Tipo: Journal Article |
Palavras-chave: SACCHAROMYCES CEREVISIAE. |
| Ano: 2008 |
URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2008a3eb742f&uri=/notices/prodinra1/2010/09/ |
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| Legras, J.L.; Merdinoglu, D.; Cornuet, J.M.; Karst, F.. |
| Fermented beverages and foods have played a significant role in most societies worldwide for millennia. To better understand how the yeast species Saccharomyces cerevisiae , the main fermenting agent, evolved along this historical and expansion process, we analysed the genetic diversity among 651 strains from 56 different geographical origins, worldwide. Their genotyping at 12 microsatellite loci revealed 575 distinct genotypes organized in subgroups of yeast types, i.e. bread, beer, wine, sake. Some of these groups presented unexpected relatedness: Bread strains displayed a combination of alleles intermediate between beer and wine strains, and strains used for rice wine and sake were most closely related to beer and bread strains. However, up to 28% of... |
| Tipo: Journal Article |
Palavras-chave: BIERE; INFERENCE BAYESIENNE DOMESTICATION; MICROSATELLITE; POPULATION GENETICS; SACCHAROMYCES CEREVISIAE; BAYESIAN INFERENCE. |
| Ano: 2007 |
URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20083bedc889&uri=/notices/prodinra1/2008/08/ |
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| Cambon, B.; Monteil, V.; Remize, F.; Camarasa, C.; Dequin, S.. |
| The utilization of Saccharomyces cerevisiae strains overproducing glycerol and with a reduced ethanol yield is a potentially valuable strategy for producing wine with decreased ethanol content. However, glycerol overproduction is accompanied by acetate accumulation. In this study, we evaluated the effects of the overexpression of GPD1, coding for glycerol-3-phosphate dehydrogenase, in three commercial wine yeast strains in which the two copies of ALD6 encoding the NADP(+)-dependent Mg2+-activated cytosolic acetaldehyde dehydrogenase have been deleted. Under wine fermentation conditions, the engineered industrial strains exhibit fermentation performance and growth properties similar to those of the wild type. Acetate was produced at concentrations similar... |
| Tipo: Journal Article |
Palavras-chave: GLYCEROL PRODUCTION; ALCOHOLIC FERMENTATION; ACETATE FORMATION; NADH; SACCHAROMYCES CEREVISIAE; WINE YEAST. |
| Ano: 2006 |
URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2007862704cb&uri=/notices/prodinra1/2009/02/ |
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| Lagorce, A.; Hauser, N.C.; Labourdette, D.; Rodriguez, C.; Martin-Yken, H.; Arroyo, J.; Hobeisel, J.D.; François, J.. |
| Perturbations of the yeast cell wall trigger a repair mechanism that reconfigures its molecular structure to preserve cell integrity. To investigate this mechanism, we compared the global gene expression in five mutant strains, each bearing a mutation (i.e. fks1, kre6, mnn9, gas1, and knr4 mutants) that affects in a different manner the cell wall construction. Altogether, 300 responsive genes were kept based on high stringency criteria during data processing. Functional classification of these differentially expressed genes showed a substantial subset of induced genes involved in cell wall construction and an enrichment of metabolic, energy generation, and cell defense categories, whereas families of genes belonging to transcription, protein synthesis, and... |
| Tipo: Journal Article |
Palavras-chave: SACCHAROMYCES CEREVISIAE; TECHNIQUE ANALYTIQUE; STRUCTURE; EXPRESSION DES GENES; MICROARRAY; ACTIVITE ENZYMATIQUE. |
| Ano: 2003 |
URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PUB0400023382102223&uri=/notices/prodinra1/2010/11/ |
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| Registros recuperados: 25 | |
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