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Registros recuperados: 25
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A novel lipase / acyltransferase from the yeast Candida albicans: expression and characterisation of the recombinant enzyme Inra
Roustan, J.L.; Rascon Chu, A.; Moulin, G.; Bigey, F..
A gene encoding an extracellular lipase (CaLIP4) from Candida albicans was successfully expressed in Saccharomyces cerevisiae after mutagenesis of its unusual CUG serine codon into a universal one. The ability of this lipase, which shares 60% sequence homology with the lipase/acyltransferase from Candida parapsilosis, to synthesise esters was investigated. CaLIP4 behaved as a true lipase, displaying activity towards insoluble triglycerides and having no activity in the presence of short-chain fatty acid (FA) esters and phosphatidylcholine. Methyl, ethyl and propyl esters were efficiently used. The lipase exhibited highest selectivity for unsaturated FA. With saturated FAs, C14-C16 acyl chains were preferred. In a biphasic aqueous/lipid system, CaLIP4...
Tipo: Journal Article Palavras-chave: CANDIDA ALBICANS; CANDIDA PARAPSILOSIS; SACCHAROMYCES CEREVISIAE; ALCOHOLYSIS ACTIVITY; LIPASE/ACYLTRANSFERASE; KINETIC CONTROL.
Ano: 2005 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20084762bbfc&uri=/notices/prodinra1/2010/09/
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Acetaldehyde addition throughout the growth phase alleviates the phenotypic effect of zinc deficiency in Saccharomyces cerevisiae Inra
Cheraiti, N.; Sauvage, F.X.; Salmon, J.M..
During experiments to determine the effects of exogenously added acetaldehyde on pure cultures of various yeast strains, we discovered that an early acetaldehyde perfusion during the growth phase allowed several yeasts to partially overcome the phenotypic effects of zinc depletion during alcoholic fermentation. We, therefore, performed genome-wide expression and proteomic analysis on an industrial Saccharomyces cerevisiae yeast strain (VL1) growing in zinc-replete or zinc-depleted conditions in the presence of perfused acetaldehyde to identify molecular markers of this effect. Zinc depletion severely affects ethanol production and therefore nicotinamide adenine dinucleotide (NAD) regeneration, although we observed partial compensation by the upregulation...
Tipo: Journal Article Palavras-chave: SACCHAROMYCES CEREVISIAE.
Ano: 2008 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2008a3eb742f&uri=/notices/prodinra1/2010/09/
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Analysis of the genomic response of a wine yeast to rehydration and inoculation Inra
Rossignol, T.; Postaire, O.; Storaï, J.; Blondin, B..
We used DNA microarrays to study the transcriptome of a wine yeast before and after rehydration and during the first hours following inoculation of a synthetic must. There was a substantial transcriptional remodeling during this period, including 1,874 genes regulated more than threefold. Dried yeasts displayed an expression profile typical of respiratory-grown cells starved for nitrogen and carbon and which had been highly stressed. During rehydration, many genes involved in biosynthetic pathways, in transcription or in protein synthesis were coordinately induced while genes subject to glucose repression were down-regulated. The transcriptional response was very rapid indicating that yeast quickly recovered the capacity to sense environmental signals and...
Tipo: Journal Article Palavras-chave: INOCULATION; REHYDRATATION; MOUT; SACCHAROMYCES CEREVISIAE.
Ano: 2006 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2008290e2419&uri=/notices/prodinra1/2010/09/
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Assimiliation of grape phytosterols by Saccharomyces cerevisiae and their impact on enological fermentations Inra
Luparia, V.; Soubeyrand, V.; Berges, T.; Julien, A.; Salmon, J.M..
Although yeasts are known to be able toincorporate a wide variety of exogenous sterols understrict anaerobiosis, no data are available on the assimilationof grapevine phytosterols under enological conditionsand the eventual impact on fermentation kinetics. We usedtherefore a mixture of pure phytosterols, in a proportionrepresentative of the different grape skins phytosterols, tosupplement a synthetic fermentation medium simulating agrape must. Under anaerobiosis, normal biomass formationwas achieved with 5 mg phytosterols l−1. Similarresults were obtained in comparison with the observedmaximal fermentation rates. These results clearly indicatedthat grape phytosterols may efficiently act as a substitutefor ergosterol in the yeast membrane for promoting...
Tipo: Journal Article Palavras-chave: OENOLOGIE VEGETALE; PHYSIOLOGIE; CINETIQUE; SACCHAROMYCES CEREVISIAE.
Ano: 2004 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PUB0400025093104737&uri=/notices/prodinra1/2010/09/
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Association between competition and obligate mutualism in a chemostat Inra
El Hajji, M.; Harmand, J.; Chaker, H.; Lobry, C..
In this paper, we consider a simple chemostat model involving two obligate mutualistic species feeding on a limiting substrate. Systems of differential equations are proposed as models of this association. A detailed qualitative analysis is carried out. We show the existence of a domain of coexistence, which is a set of initial conditions in which both species survive. We demonstrate, under certain supplementary assumptions, the uniqueness of the stable equilibrium point which corresponds to the coexistence of the two species.
Tipo: Journal Article Palavras-chave: SURVIE DES ESPECES; COMPORTEMENT ASYMPTOTIQUE; EQUATION DIFFERENTIELLE; INTERACTION MICROBIOLOGIQUE MICROBIAL INTERACTIONS; MICROBIAL ECOLOGY; YEAST; SACCHAROMYCES CEREVISIAE; CHEMOSTAT; COMPETITION; MUTUALISM; ASYMPTOTIC BEHAVIOUR; COEXISTENCE; DULAC CRITERION; THIEME’S RESULT; ;  .
Ano: 2009 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD201035ecf34a&uri=/notices/prodinra1/2010/12/
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Bread, beer and wine: Saccharomyces cerevisiae diversity reflects human history Inra
Legras, J.L.; Merdinoglu, D.; Cornuet, J.M.; Karst, F..
Fermented beverages and foods have played a significant role in most societies worldwide for millennia. To better understand how the yeast species Saccharomyces cerevisiae , the main fermenting agent, evolved along this historical and expansion process, we analysed the genetic diversity among 651 strains from 56 different geographical origins, worldwide. Their genotyping at 12 microsatellite loci revealed 575 distinct genotypes organized in subgroups of yeast types, i.e. bread, beer, wine, sake. Some of these groups presented unexpected relatedness: Bread strains displayed a combination of alleles intermediate between beer and wine strains, and strains used for rice wine and sake were most closely related to beer and bread strains. However, up to 28% of...
Tipo: Journal Article Palavras-chave: BIERE; INFERENCE BAYESIENNE DOMESTICATION; MICROSATELLITE; POPULATION GENETICS; SACCHAROMYCES CEREVISIAE; BAYESIAN INFERENCE.
Ano: 2007 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20083bedc889&uri=/notices/prodinra1/2008/08/
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Cloning of a cDNA encoded by a member of the Arabidopsis thaliana ATP sulfurylase multigene family. Expression studies in yeast and in relation to plant sulfur nutrition Inra
Logan, H.M.; Cathala, N.; Grignon, C.; Davidian, J.C..
Tipo: Journal Article Palavras-chave: ARABIDOPSIS THALIANA; ATP; SULFURYLASE; CLONAGE MOLECULAIRE; ADNC; SEQUENCE NUCLEOTIDIQUE; SACCHAROMYCES CEREVISIAE; SEQUENCE HOMOLOGUE; ACTIVITE ENZYMATIQUE; TRANSCRIPTION; SULFATE; BRASSICA NAPUS.
Ano: 1996 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PUB0200029830090142&uri=/notices/prodinra1/2010/09/
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Cofactor engineering in Saccharomyces cerevisiae: Expression of a H2O-forming NADH oxidase and impact on redox metabolism Inra
Heux, S.; Cachon, R.; Dequin, S..
The pyridine nucleotides NAD(H) and NADP(H) play major roles in the formation of by-products. To analyse how Saccharomyces cerevisiae (S. cerevisiae) metabolism during growth on glucose might be altered when intracellular NADH pool is decreased, we expressed noxE encoding a water-forming NADH oxidase from Lactococcus lactis (L. lactis) in the S. cerevisiae strain V5. During batch fermentation under controlled microaeration conditions, expression of the NADH oxidase under the control of a yeast promoter lead to large decreases in the intracellular NADH concentration (five-fold) and NADH/NAD+ ratio (six-fold). This increased NADH consumption caused a large redistribution of metabolic fluxes. The ethanol, glycerol, succinate and hydroxyglutarate yields were...
Tipo: Journal Article Palavras-chave: SACCHAROMYCES CEREVISIAE; COFACTOR ENGINEERING; NADH OXIDASE; METABOLITE YIELDS; MICROAEROBIC GROWTH.
Ano: 2006 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD200735919da0&uri=/notices/prodinra1/2008/04/
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Comparison of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and PCR-RAPD as potential tools to differentiate SACCHAROMYCES CEREVISIAE strains. Repositório Alice
AUGUSTINI, B. C.; CANOSSA, S.; MANFROI, V.; SILVA, G. A. da.
2015
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Industria do vinho; VINHO; SACCHAROMYCES CEREVISIAE.
Ano: 2015 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1028042
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Cross effects of the strain of dietary Saccharomyces cerevisiae and rearing conditions on the onset of intestinal microbiota and digestive enzymes in rainbow trout, Onchorhynchus mykiss, fry Inra
Waché, Y.; Auffray, F.; Gatesoupe, J.F.; Zambonino, J.; Gayet, V.; Labbé, L.; Quentel, C..
Tipo: Journal Article Palavras-chave: DEVELOPPEMENT LARVAIRE; SACCHAROMYCES CEREVISIAE.
Ano: 2006 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20074638afdf&uri=/notices/prodinra1/2007/11/
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Effects of GPD1 overexpression in Saccharomyces cerevisiae commercial wine yeast strains lacking ALD6 genes Inra
Cambon, B.; Monteil, V.; Remize, F.; Camarasa, C.; Dequin, S..
The utilization of Saccharomyces cerevisiae strains overproducing glycerol and with a reduced ethanol yield is a potentially valuable strategy for producing wine with decreased ethanol content. However, glycerol overproduction is accompanied by acetate accumulation. In this study, we evaluated the effects of the overexpression of GPD1, coding for glycerol-3-phosphate dehydrogenase, in three commercial wine yeast strains in which the two copies of ALD6 encoding the NADP(+)-dependent Mg2+-activated cytosolic acetaldehyde dehydrogenase have been deleted. Under wine fermentation conditions, the engineered industrial strains exhibit fermentation performance and growth properties similar to those of the wild type. Acetate was produced at concentrations similar...
Tipo: Journal Article Palavras-chave: GLYCEROL PRODUCTION; ALCOHOLIC FERMENTATION; ACETATE FORMATION; NADH; SACCHAROMYCES CEREVISIAE; WINE YEAST.
Ano: 2006 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2007862704cb&uri=/notices/prodinra1/2009/02/
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Engineering a Saccharomyces cerevisiae wine yeast that exhibits reduced ethanol production during fermentation under controlled microoxygenation conditions Inra
Heux, S.; Sablayrolles, J.M.; Cachon, R.; Dequin, S..
We recently showed that expressing an H2O-NADH oxidase in Saccharomyces cerevisiae drastically reduces the intracellular NADH concentration and substantially alters the distribution of metabolic fluxes in the cell. Although the engineered strain produces a reduced amount of ethanol, a high level of acetaldehyde accumulates early in the process (1 g/liter), impairing growth and fermentation performance. To overcome these undesirable effects, we carried out a comprehensive analysis of the impact of oxygen on the metabolic network of the same NADH oxidase-expressing strain. While reducing the oxygen transfer rate led to a gradual recovery of the growth and fermentation performance, its impact on the ethanol yield was negligible. In contrast, supplying oxygen...
Tipo: Journal Article Palavras-chave: WINE YEAST; SACCHAROMYCES CEREVISIAE; NADPH; MICROOXYGENATION; ETHANOL.
Ano: 2006 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20079f1a58eb&uri=/notices/prodinra1/2008/04/
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Genetic analysis of Saccharomyces cerevisiae strains isolated from Palm Wine in Eastern Nigeria. Comparison with other African strains. Inra
Ezeronye, O.U.; Legras, J.L..
Aims: To study the yeast diversity of Nigerian palm wines by comparison with other African strains. Methods and Results: Twenty-three Saccharomyces cerevisiae strains were obtained from palm wine samples collected at four locations in eastern Nigeria, and characterized using different molecular techniques: internal transcribed spacer restriction fragment length polymorphism and sequence analysis, pulsed field gel electrophoresis, inter delta typing and microsatellite multilocus analysis. These techniques revealed that palm wine yeasts represent a group of closely related strains that includes other West African isolates (CBS400, NCYC110, DVPG6044). Population analysis revealed an excess of homozygote strains and an allelic richness similar to...
Tipo: Journal Article Palavras-chave: FUNGI; MICROBIOLOGIE APPLIQUEE; POLYMORPHISME; ETUDE COMPARATIVE; VIN DE PALME; SACCHAROMYCES CEREVISIAE; GENETIQUE MICROSATELLITE; MOLECULAR POLYMORPHISM; PALM WINE; YEAST.
Ano: 2009 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD200941167abe&uri=/notices/prodinra1/2010/07/
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Genetic characterization of commercial Saccharomyces cerevisiae isolates recovered from vineyard environments Inra
Schuller, D.; Pereira, L.; Alves, H.; Cambon, B.; Dequin, S.; Casal, M..
One hundred isolates of the commercial Saccharomyces cerevisiae strain ZymafloreVL1 were recovered from spontaneous fermentations carried out with grapes collectedfrom vineyards located close to wineries in the Vinho Verde wine region ofPortugal. Isolates were differentiated based on their mitochondrial DNA restrictionpatterns and the evaluation of genetic polymorphisms was carried out by microsatelliteanalysis, interdelta sequence typing and pulsed-field gel electrophoresis (PFGE).Genetic patterns were compared to those obtained for 30 isolates of the original commercializedZymaflore VL1 strain. Among the 100 recovered isolates we found a highpercentage of chromosomal size variations, most evident for the smaller chromosomesIII and VI. Complete loss of...
Tipo: Journal Article Palavras-chave: SACCHAROMYCES CEREVISIAE.
Ano: 2007 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2008c4af97ba&uri=/notices/prodinra1/2008/04/
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Genome-wide analysis of the response to cell wall mutations in the yeast Saccharomyces cerevisiae Inra
Lagorce, A.; Hauser, N.C.; Labourdette, D.; Rodriguez, C.; Martin-Yken, H.; Arroyo, J.; Hobeisel, J.D.; François, J..
Perturbations of the yeast cell wall trigger a repair mechanism that reconfigures its molecular structure to preserve cell integrity. To investigate this mechanism, we compared the global gene expression in five mutant strains, each bearing a mutation (i.e. fks1, kre6, mnn9, gas1, and knr4 mutants) that affects in a different manner the cell wall construction. Altogether, 300 responsive genes were kept based on high stringency criteria during data processing. Functional classification of these differentially expressed genes showed a substantial subset of induced genes involved in cell wall construction and an enrichment of metabolic, energy generation, and cell defense categories, whereas families of genes belonging to transcription, protein synthesis, and...
Tipo: Journal Article Palavras-chave: SACCHAROMYCES CEREVISIAE; TECHNIQUE ANALYTIQUE; STRUCTURE; EXPRESSION DES GENES; MICROARRAY; ACTIVITE ENZYMATIQUE.
Ano: 2003 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PUB0400023382102223&uri=/notices/prodinra1/2010/11/
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Interaction between yeast Sgs1 helicase and DNA Topoisomerse III Inra
Bennett, R.J.; Noirot-Gros, M.F.; Wang, J.C..
Tipo: Journal Article Palavras-chave: SACCHAROMYCES CEREVISIAE; HELICASE; ADN TOPOISOMERASE; CHROMATOGRAPHIE D'AFFINITE; TECHNIQUE ANALYTIQUE.
Ano: 2000 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PUB0300012684095402&uri=/notices/prodinra1/2010/09/
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Inventory and monitoring of wine microbial consortia Inra
Renouf, V.; Claisse, O.; Lonvaud-Funel, A..
The evolution of the wine microbial ecosystem is generally restricted to Saccharomyces cerevisiae and Oenococcus oeni, which are the two main agents in the transformation of grape must into wine by acting during alcoholic and malolactic fermentation, respectively. But others species like the yeast Brettanomyces bruxellensis and certain ropy strains of Pediococcus parvulus can spoil the wine. The aim of this study was to address the composition of the system more precisely, identifying other components. The advantages of the polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) approach to wine microbial ecology studies are illustrated by bacteria and yeast species identification and their monitoring at each stage of wine production....
Tipo: Journal Article Palavras-chave: SACCHAROMYCES CEREVISIAE; ASCOMYCOTINA; ENDOMYTALES; SACCHAROMYCES; OENOCOCCUS OENI; BRETTANOMYCES BRUXELLENSIS; PEDIOCOCCUS PARVULUS; BACTERIA; STREPTOCOCCACEAE; PEDIOCOCCUS; PCR-DGGE; INVENTAIRE; IDENTIFICATION.
Ano: 2007 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20077b2366db&uri=/notices/prodinra1/2010/09/
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Iron uptake and storage in plants Inra
Briat, J.F.; Arnaud, N.; Cellier, F.; Curie, C.; Gaymard, F.; Ravet, K.; Seguela, M.; Vert, G..
Tipo: Journal Article Palavras-chave: FERRIC CHELATE; METAL TRANSPORT; IRON; ROOT; SACCHAROMYCES CEREVISIAE; FERRIN GENE EXPRESSION; IRT1.
Ano: 2007 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20076a9b48c7&uri=/notices/prodinra1/2007/09/
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L'ensemencement séquentiel d'une levure non Saccharomyces et d'une levure Saccharomyces cerevisiae vers de nouveaux horizons aromatiques Inra
Pillet, O.; Aguera, E.; Silvano, A.; Languet, P..
Tipo: Journal Article Palavras-chave: NON-SACCHAROMYCES; ENSEMENCEMENT; SACCHAROMYCES CEREVISIAE; CHARDONNAY; TORULASPORA DELBRUECKII.
Ano: 2010 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD201089d7576b&uri=/notices/prodinra1/2010/08/
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Nicotinic acid controls lactate production by K1-LDH: a Saccharomyces cerevisiae strain expressing a bacterial LDH gene Inra
Colombié, S.; Sablayrolles, J.M..
Industrial applications for lactate, such as the production of chemicals, has led to interest in producing this organic acid by metabolically engineered a yeast such as Saccharomyces cerevisiae, which is more acid tolerant than lactic acid bacteria. This paper deals with lactate production by S. cerevisiae K1-LDH, in which the Lactobacillus plantarum lactate dehydrogenase (LDH) gene is integrated into the genome of the wine yeast strain K1. We show that a vitamin, nicotinic acid (NiA), was the limiting factor for lactate production during fermentation with the K1-LDH strain. Increasing the NiA concentration in batch conditions or in the medium used to feed chemostats affected the lactate yield. Moreover, the addition of pulses of NiA or the exponential...
Tipo: Journal Article Palavras-chave: ACIDE NICOTINIQUE; L-LACTATE; LACTATE DESHYDROGENASE; SACCHAROMYCES CEREVISIAE; OENOLOGIE; EXPRESSION DES GENES NICOTINIC ACID; L-LACTATE; ALCOHOLIC FERMENTATION; LACTATE DEHYDROGENASE; SACCHAROMYCES CEREVISIAE.
Ano: 2004 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PUB0400025097104741&uri=/notices/prodinra1/2010/11/
Registros recuperados: 25
Primeira ... 12 ... Última
 

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