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| Quero, C.; Lucas, P.; Renou, M.; Guerrero, A.. |
| The major component of the sex pheromone of female Spodoptera littoralis, (Z,E)-9,11-tetradecadienyl acetate (1), elicited all steps of the male behavioral sequence, i.e., wing fanning and taking flight, oriented upwind flight and arrival to the middle of the tunnel, close approach and contact with the source. The activity was equivalent to that elicited by virgin females. In the range of doses tested, the dosage of 1 had no significant effect on the number of source contacts. Male response was significantly affected by light intensity, being optimum at 3 lux. Activity of the minor components (Z)-9-tetradecenyl acetate (2), (E)-11-tetradecenyl acetate (3), tetradecyl acetate (4), (Z)-11-tetradecenyl acetate (5), and (Z,E)-9,12-tetradecadienyl acetate (6)... |
| Tipo: Journal Article |
Palavras-chave: SPODOPTERA; LEPIDOPTERE; SENSILLE; PHEROMONE SEXUELLE; TUNNEL DE VOL WIND TUNNEL; BEHAVIOR; SINGLE SENSILLUM RECORDING; SPODOPTERA LITTORALIS; LEPIDOPTERA; NOCTUIDAE; EGYPTIAN ARMYWORM. |
| Ano: 1996 |
URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PUB9600002173058628&uri=/notices/prodinra1/2010/11/ |
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| Durand, N.; Carot-Sans, G.; Chertemps, T.; Bozzolan, F.; Party, V.; Renou, M.; Debernard, S.; Rosell, G.; Maïbèche-Coisne, M.. |
| Background: Carboxyl/cholinesterases (CCEs) are highly diversified in insects. These enzymes have a broad range of proposed functions, in neuro/developmental processes, dietary detoxification, insecticide resistance or hormone/ pheromone degradation. As few functional data are available on purified or recombinant CCEs, the physiological role of most of these enzymes is unknown. Concerning their role in olfaction, only two CCEs able to metabolize sex pheromones have been functionally characterized in insects. These enzymes are only expressed in the male antennae, and secreted into the lumen of the pheromone-sensitive sensilla. CCEs able to hydrolyze other odorants than sex pheromones, such as plant volatiles, have not been identified. Methodology: In... |
| Tipo: Journal Article |
Palavras-chave: LEPIDOPTERA; NOCTUIDAE; HEXAPODA; PCR; ADNC; SDS PAGE; EXPRESSION DES GENES; ELECTROPHORESE; CARBOXYLESTERASE; SPODOPTERA LITTORALIS; OLFACTION; ODEEUR; PLANTE HOTE; INSECTE; ENZYME; BIOLOGIE MOLECULAIRE; BIOCHIMIE; ELECTROPHYSIOLOGIE; PHEROMONE; PHEROMONE SEXUELLE; RECOMBINAISON; PROTEINE. |
| Ano: 2010 |
URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2010229c7cd1&uri=/notices/prodinra1/2011/05/ |
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| Costa, S.C.P.; Ribeiro, C.; Girard, P.A.; Zumbihl, R.; Brehélin, M.. |
| Haemocytes are the main immunocompetent cells in insect cellular immune reactions. Here, we show that in Spodoptera littoralis, granular haemocytes are the primary phagocyte haemocytes, both in vivo and in vitro. The "trigger" and "zipper" modes of engulfment known in mammal macrophages are active, in vivo, in S. littoralis granular haemocytes, together with macropinocytosis. Lipopolysaccharide as well as lipoteichoic acid inhibit the binding of both Gram-positive (Corynebacterium xerosis) and Gramnegative (Escherichia coli) bacteria on granular haemocytes. In addition, different ligands can inhibit the binding of E coli. Most of these inhibitors are known as ligands of scavenger receptors in mammal macrophages and we hypothesise that one of the receptors... |
| Tipo: Journal Article |
Palavras-chave: SPODOPTERA LITTORALIS; NOCTUELLE MACROPINOCYTOSIS; TRIGGER; ZIPPER; RECEPTOR; INSECT. |
| Ano: 2005 |
URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD201069bfa10d&uri=/notices/prodinra1/2011/05/ |
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| Chouquet, B.; Lucas, P.; Bozzolan, F.; Solvar, M.; Maïbèche-Coisné, M.; Durand, N.; Debernard, S.. |
| To clarify the role of phospholipase C (PLC) in insect olfactory transduction, we have undertaken its molecular identification in the moth Spodoptera littoralis. From the analysis of a male antennal expressed sequence tag library, we succeeded in cloning a full-length cDNA encoding a PLC that belongs to the cluster of PLC-b subtypes. In adult males, the PLC-b transcript was located predominantly in brain and antennae where its presence was detected in the olfactory sensilla trichodea. Moreover, PLC-b was expressed in antennae at the beginning of the pupal stage, then reached a maximum at the end of this stage and was maintained at this level during the adult period. Taken together, these results provided molecular evidence for the putative participation of... |
| Tipo: Journal Article |
Palavras-chave: INSECTE; PHOSPHOLIPASE; OLFACTION; ODEUR; MITE; TECHNIQUE ANALYTIQUE; SEQUENCE NUCLEOTIDIQUE; EXPRESSION DES GENES; ADNC; TRANSCRIPTION; NORTHERN BLOT; ELECTROPHORESE; PCR; CLONAGE MOLECULAIRE; ANALYSE PHYLOGENETIQUE; LEPIDOPTERA; NOCTUELLE MEDITERRANEENNE EXPRESSION PATTERNS; OLFACTORY SYSTEM; PHOSPHOLIPASE C; SPODOPTERA LITTORALIS; SIGNAL TRANSDUCTION. |
| Ano: 2010 |
URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20108d7c7c75&uri=/notices/prodinra1/2010/09/ |
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| Abd-Alla, A.; Jousset, O.X.; Li, Y.; Fédière, G.; Cousserans, F.; Bergoin, M.. |
| The genome of Junonia coenia densovirus (JcDNV) shares with members of the genus Densovirus the property of possessing structural (VP) and nonstructural (NS) genes in opposite orientations. The three NS genes located in the 5' half on one strand encode three NS proteins assumed to be involved in viral DNA replication: NS-1 and NS-2, which are common to all DNVs, and a 28-kDa polypeptide, NS-3, with a unique sequence. Whereas the essential role played by JcDNV NS-1 in viral DNA replication has been clearly established (C. Ding, M. Urabe, M. Bergoin, and R. M. Kotin, J. Virol. 76:338-345, 2002), nothing is known of the biological function(s) of NS-3. To investigate this function, we designed constructs derived from pBRJ, a plasmid encompassing an infectious... |
| Tipo: Journal Article |
Palavras-chave: JUNONIA COENICA; SPODOPTERA LITTORALIS; DENSOVIRUS; VIRAL DNA REPLICATION. |
| Ano: 2004 |
URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2008cdec6062&uri=/notices/prodinra1/2009/03/ |
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| Party, V.; Hanot, C.; Said, I.; Rochat, D.; Renou, M.. |
| In moths, the components of the female pheromone blend are detected in the male antennae by pheromone olfactory receptor neurons (Ph-ORNs) expressing narrowly tuned olfactory receptors. Responses to sex pheromones have generally been thought to be independent from the odorant background. However, interactions between pheromone components and plant volatiles have been reported at behavioral and detection levels. To document the mechanisms of such interactions, we analyzed Ph-ORN responses of Spodoptera littoralis to the main pheromone component, Z9E11-14:Ac, in the presence of 4 monoterpenes. To mimic natural contexts in which plant odors and pheromone emanate from different sources, the 2 stimuli were presented with different temporal patterns and from... |
| Tipo: Journal Article |
Palavras-chave: SPODOPTERA LITTORALIS; NOCTUIDAE; ARTHROPODA; HEXAPODA; INSECTE; LEPIDOPTERA; PLANTE; COMPOSE VOLATILE; MITE; MONOTERPENE; PHEROMONE; ATTRACTIF SEXUEL; ODEUR ODORANT INTERACTIONS; OLFACTION; OLFACTORY RECEPTOR NEURONS; PLANT VOLATILE COMPOUNDS. |
| Ano: 2009 |
URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2010e254489a&uri=/notices/prodinra1/2010/10/ |
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