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Molecular cloning and ultrastructural localization of the core protein of an eggshell matrix proteoglycan, ovocleidin-116 Inra
Hincke, M.T.; Gautron, J.; Tsang, C.P.W.; McKee, M.D.; Nys, Y..
The role of avian eggshell matrix proteins in shell formation is poorly understood. This calcitic biomaterial forms in a uterine fluid where the protein composition varies during the initial, calcification, and terminal phases of eggshell deposition. A specific antibody was raised to a 116-kDa protein, which is most abundant in uterine fluid during active eggshell calcification. This antiserum was used to expression screen a bacteriophage cDNA library prepared using mRNA extracted from pooled uterine tissue harvested at the midpoint of eggshell calcification. Plasmids containing inserts of differing 5′-lengths were isolated with a maximum cDNA sequence of 2.4 kilobases. Northern blotting and reverse transcriptase-polymerase chain reaction demonstrated that...
Tipo: Journal Article Palavras-chave: RECHERCHE AVICOLE; OEUF; CLONAGE MOLECULAIRE; EXPRESSION DES GENES; TECHNIQUE ANALYTIQUE; TRANSCRIPTION; SEQUENCE NUCLEOTIDIQUE; COQUILLE D'OEUF; ULTRASTRUCTURE; STRUCTURE MINERALE; OVOCLEIDINE; PROTEOGLYCANE; MICROSCOPIE ELECTRONIQUE.
Ano: 1999 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PUB0300012762095479&uri=/notices/prodinra1/2010/10/
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