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Registros recuperados: 33
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Bioprotective Effect of Lactococcus piscium CNCM I-4031 Against Listeria monocytogenes Growth and Virulence ArchiMer
Saraoui, Taous; Leroi, Francoise; Chevalier, Frederique; Cappelier, Jean-michel; Passerini, Delphine; Pilet, Marie-france.
Listeria monocytogenes is a Gram-positive pathogen occurring in many refrigerated ready-to-eat foods. It is responsible for foodborne listeriosis, a rare but severe disease with a high mortality rate (20–30%). Lactococcus piscium CNCM I-4031 has the capacity to prevent the growth of L. monocytogenes in contaminated peeled and cooked shrimp and in a chemically defined medium using a cell-to-cell contact-dependent mechanism. To characterize this inhibition further, the effect of L. piscium was tested on a collection of 42 L. monocytogenes strains. All strains were inhibited but had different sensitivities. The effect of the initial concentration of the protective and the target bacteria revealed that the inhibition always occurred when L. piscium had reached...
Tipo: Text Palavras-chave: Biopreservation; Lactococcus piscium; Listeria monocytogenes; Co-culture; Cell ratio; Scanning electron microscopy; Virulence.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00449/56065/57588.pdf
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Characterization of neutrophil adhesion to different titanium surfaces. Repositório Alice
CAMPOS, V.; MELO, R. C. N.; SILVA, L. P. da; AQUINO, E. N.; CASTRO, M. S.; FONTES, W..
bitstream/item/179993/1/CAMPOS2014-Article-CharacterizationOfNeutrophilAd.pdf
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Titanium surfaces; Neutrophil morphology; Adhesion molecules; Inflammatory response; Flow cytometry; Scanning electron microscopy.
Ano: 2014 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/994650
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CHORDAE TENDINEAE ARCHITECTURE IN THE PAPILLARY MUSCLE INSERTION International Journal of Morphology
Wang Gusukuma,Luciana; Prates,José Carlos; Smith,Ricardo Luiz.
Chordae tendineae architecture and their junction with papillary muscle were studied with scanning electron microscopy, comparing human and swine hearts. Papillary muscles with the corresponding chordae tendineae were obtained from human and pig (Sus scrofa) hearts after exposition of the left ventricle cavity, fixed with 4% formaldehyde. The specimens were sectioned in small samples and divided in four groups, a control one and the others submitted to digestion in a solution of 2.5% sodium hypochlorite in saline solution during 5, 10 and 15 minutes respectively. The specimens were washed and fixed in 1% osmium tetroxide in cacodylate buffer, kept in 1% tannic acid for 30 minutes, dehydrated and critical point dried, mounted and sputtered with gold. The...
Tipo: Journal article Palavras-chave: Chordae tendineae; Architecture; Human; Pigs; Scanning electron microscopy.
Ano: 2004 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022004000400005
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Comparative Scanning Electron Microscopy of Platelets and Fibrin Networks of Human and Differents Animals International Journal of Morphology
Pretorius,E; Vieira,W. A; Oberholzer,H. M; Auer,R. E. J.
The purpose of the present study was to compare the platelet and fibrin network ultrastructure of humans to eight different animal species in order to determine the differences between human and animal platelet and fibrin morphology, and to determine whether the animals studied differ in their platelet and fibrin morphology, and whether these differences can be observed by scanning electron microscopy. Platelets and fibrin networks play an important role both in the coagulation process as well as physiologically in allergic processes and immunological mechanisms. The thickness of human fibrin networks were compared to mouse (Mus musculus), equine (Equus caballus), vervet monkey (Chlorocebus aethiops previously Cercopithecus aethiops), oryx (Oryx gazella),...
Tipo: Journal article Palavras-chave: Scanning electron microscopy; Platelets; Fibrin networks; Human; Animals.
Ano: 2009 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022009000100013
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Comparative Wood Anatomy of the Primuloid Clade (Ericales s.l.) Naturalis
Lens, F.; Jansen, S.; Caris, P.; Serlet, L.; Smets, E..
The wood structure of 78 species from 27 genera representing the woody primuloids (Maesaceae, Myrsinaceae, and Theophrastaceae) was investigated using light microscopy (LM) and scanning electron microscopy (SEM). Results indicated that the ray structure, the nature of mineral inclusions, and the occurrence of breakdown areas in rays can be used to separate the three primuloid families from each other. Within Ericales, the presence of exclusively multiseriate rays is synapomorphic for Myrsinaceae and Theophrastaceae, and the occurrence of breakdown areas in rays is synapomorphic for Myrsinaceae. Within Myrsinaceae, the wood structure of the mangrove genus Aegiceras differs because it has short vessel elements that are storied, non-septate fibers, a...
Tipo: Article / Letter to the editor Palavras-chave: Primuloids; Ericales s.l.; Maesaceae; Myrsinaceae; Theophrastaceae; Wood anatomy; Comparison; Light microscopy; Scanning electron microscopy.
Ano: 2005 URL: http://www.repository.naturalis.nl/record/407307
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Cytokinin preconditioning enhances multiple shoot regeneration in Pongamia pinnata (L.) Pierre - a potential, non-edible tree seed oil source for biodiesel Electron. J. Biotechnol.
Belide,Srinivas; Sajjalaguddam,Ramgopal Rao; Paladugu,Anitha.
An efficient, highly reproducible protocol for multiple shoot induction and plant regeneration of Pongamia pinnata has been successfully developed using cotyledonary node explants. This study also demonstrates that preconditioning of explant stimulates production of multiple shoots from cotyledonary nodes of P. pinnata. The highest direct shoot regeneration (90%) with an average of 18.4 � 3.1 shoots/explant were obtained when cotyledonary node explants were excised from seedlings germinated on Murashige and Skoog (MS) media supplemented with benzyladenine (BA) 1 mg l-1, and subsequently cultured on MS media with 1 mgl-1 thidiazuron (TDZ). Scanning electron microscope observations of cotyledonary node (CN) explants excised from pre-conditioned and normal...
Tipo: Journal article Palavras-chave: Benzyladenine; Cotyledonary node; Multiple shoot induction; Scanning electron microscopy; Thidiazuron.
Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000600003
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Ecophysiological, anatomical and ultrastructural characteristics of Vitex polygama Cham. (Verbenaceae) submitted to different concentrations of fluoride Biological Sciences
Rossi, Zélia Terezinha Teixeira; Castro, Evaristo Mauro de; Pereira, Giuslan Carvalho; Barbosa, João Paulo Rodrigues Alves Delfino; Carvalho, Graziele Wolff de Almeida; Rossi, Marco Aurélio de Lima Silva; Pereira, Fabrício José.
Fluorides released during the manufacture of aluminum, fertilizers, glass and ceramics are considered to be highly toxic to plants, causing damages in relatively small concentrations. This study aimed to evaluate fluoride (F) accumulation and its effects on Vitex polygama Cham. (Verbenaceae) exposed to simulated rain containing potassium fluoride in concentrations of 5, 10, 15 and 20 mg L-1. Vitex polygama presented no visual symptoms of foliar injury and accumulated a relatively small amount of F-, significant only for the treatments with higher concentrations of the pollutant.  F- promoted an increase in non-photochemical quenching (qN) and in the coefficient due to non-photochemical extinction (NPQ), which indicates higher dissipation of radiant energy...
Tipo: Info:eu-repo/semantics/article Palavras-chave: 2.03.00.00-0; 2.03.02.03-7; 2.03.03.00-9 chlorophyll a fluorescence; Potassium fluoride; Scanning electron microscopy; Transmission electron microscopy; Biomonitoring studies Botânica; Anatomia Vegetal; Fisiologia Vegetal.
Ano: 2016 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/31400
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Effect of morphological heterogeneity of somatic embryos of Melia azedarach on conversion into plants Biocell
Vila,Silvia; Gonzalez,Ana; Rey,Hebe; Mroginski,Luis.
Embryogenic cultures were initiated from immature Melia azedarach (Meliaceae) zigotic embryos. Explants were induced on Murashige and Skoog (1962) medium with 4.54 μM thidiazuron or 0.45 μM dichlorophenoxyacetic acid. After 6 weeks of culture on induction medium, somatic embryos were categorized in four morphological classes based on the presence of single or fused embryos and if they remained united or not to the original explant; that were evaluated histologically. The somatic embryos of every category were transferred, in groups or individually, on a 1/4 MS medium. Bipolar embryos, the more typically normal ones, had well defined shoot and root apical meristems and produced single plants; subcultured individually their conversion was...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Somatic embryo morphology; Conversion; Histology; Scanning electron microscopy.
Ano: 2010 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452010000100002
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Effect of ultrasonication of Switchgrass on fermentable sugar production and biomass physical structure CIGR Journal
Sharma, Rajat; Sharma-Shivappa, Ratna R.; Boyette, Michael D.; Stikeleather, Larry F..
Pretreatment of lignocellulosic biomass for enhancing sugar yields has been studied extensively over the years.  Conventional methods employed to preprocess biomass to make it conducive for enzymatic hydrolysis have been hampered by issues like poor energy efficiency and production of undesirable by-products.  Ultrasonication, which involves the treatment of biomass through ultrasonic sound waves in a liquid medium without additional chemicals, is believed to have potential for biomass pretreatment.  In this study the effects of ultrasonication on switchgrass, a potential feedstock for bioethanol production due to its high cellulosic content, were investigated.  Results of compositional analysis and scanning electron microscopy conducted to visualize...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Panicum virgatum; Amplitude; Pretreatment; Hydrolysis; Scanning electron microscopy.
Ano: 2013 URL: http://www.cigrjournal.org/index.php/Ejounral/article/view/2549
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Etude du biofilm microalgal des vasières intertidales : dynamique spatio-temporelle à micro-échelle et performances photosynthétiques ArchiMer
Herlory, Olivier.
The microalgal biofilm, which is present on sediment surface, is the primary production unit on bare intertidal mudlfats. In order to improve our understanding of this primary production system, a study was undertaken at microscale to analyse the structural and functional properties of this biofilm. In the first part of this work, a detailed descriptive analysis lead to the characterisation of the spatiotemporal dynamics of the physical structure of the biofilm. It turns out that the biofilm exhibits its own dynamics at the sediment surface (as a discrete compartment in the top 200 µm), but still has a functional relationship with the total available biomass (PCB) distributed within the top 1 cm (linear relationship between the maximum size of the biofilm...
Tipo: Text Palavras-chave: PAM Fluorometry; Scanning electron microscopy; Photosynthesis; Spatio temporal dynamic; Micro scale; Microphytobenthic biofilm; Intertidal mudflats; Fluorimétrie PAM; Microscopie électronique à balayage; Photosynthèse; Dynamique spatio temporelle; Micro échelle; Biofilm microphytobenthique; Vasière intertidal.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/these-2138.pdf
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Extrato de casca de café, óleo essencial de tomilho e acibenzolar-S-metil no manejo da cercosporiose-do-cafeeiro. Repositório Alice
PEREIRA, R.B.; ALVES, E.; RIBEIRO JÚNIOR, P.M.; RESENDE, M.L.V. de; LUCAS, G.C.; FERREIRA, J.B..
O objetivo deste trabalho foi avaliar o efeito de concentrações de extrato de casca de café, óleo essencial de tomilho e acibenzolar-S-metil na germinação, no crescimento micelial e no desenvolvimento in vivo de Cercospora coffeicola, além de caracterizar a eficiência deles como indutores de resistência, e determinar a atividade da enzima peroxidase e o acúmulo de lignina nos tecidos de cafeeiro. O extrato de casca de café não afetou a germinação, entretanto, inibiu o crescimento micelial proporcionalmente ao aumento das concentrações. O óleo essencial de tomilho inibiu a germinação e o crescimento micelial com o aumento das concentrações. O extrato de casca de café e o acibenzolar-S-metil não afetaram a germinação nem o desenvolvimento dos tubos...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Cercospora coffeicola; Coffea arabica; Indução de resistência; Microscopia eletrônica de varredura; Resistance induction; Scanning electron microscopy.
Ano: 2008 URL: http://www.alice.cnptia.embrapa.br/handle/doc/122214
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Floral development in three species of Impatiens (Balsaminaceae) Naturalis
Caris, P.L.; Geuten, K.P.; Janssens, S.B.; Smets, E..
The floral morphological and developmental patterns in three species of Impatiens (Balsaminaceae), namely I. columbaria, I. hawkeri, and I. niamniamensis, were studied to contribute to a better understanding of floral evolution in the genus. Strangely enough, the highly diverse floral morphology and ontogeny of this horticulturally important genus have never been studied thoroughly (e.g., using scanning electron microscopic techniques). We discuss the position and the developmental sequence of the different perianth members. We hypothesized that in the course of evolution, the anterolateral sepals become reduced and that a morphocline can be recognized going from species with five sepals, over species with rudimentary sepals that fuse postgenitally with...
Tipo: Article / Letter to the editor Palavras-chave: Balsaminaceae; Ericales; Floral development; Floral evolution; Floral morphology; Impatiens; Scanning electron microscopy; 42.40.
Ano: 2006 URL: http://www.repository.naturalis.nl/record/422332
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Floral Ontogeny in Ficinia and Isolepis (Cyperaceae), with Focus on the Nature and Origin of the Gynophore Naturalis
Vrijdaghs, H.; Goetghebeur, P.; Muasya, A.M.; Caris, P.; Smets, E..
! Background and Aims The generic delimitations of Ficinia and Isolepis, sister genera in the Cypereae, are blurred. Typical Ficinia flowers have a lobed gynophore, which envelops the base of the nutlet, whereas in Isolepis the character is considered to be absent. Some former species of Isolepis, lacking the gynophore, were recently included in Ficinia. The floral ontogeny of representative taxa in Ficinia and Isolepis were investigated with the aim of evaluating the origin and nature of the gynophore in the Cypereae. ! Methods The spikelet and floral ontogeny in inflorescences collected in the field was investigated using scanning electron microscopy (SEM) and light microscopy (LM). ! Key Results SEM images of Isolepis setacea and I. antarctica, Ficinia...
Tipo: Article / Letter to the editor Palavras-chave: Ficinia; Floral ontogeny; Gynophore; Isolepis; Scanning electron microscopy.
Ano: 2005 URL: http://www.repository.naturalis.nl/record/407286
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Floral Ontogeny in Scirpus, Eriophorum and Dulichium (Cyperaceae), with Special Reference to the Perianth Naturalis
Vrijdaghs, A.; Caris, P.; Goetghebeur, P.; Smets, E..
Background and Aims Based on molecular phylogenetic analysis, it has been suggested recently that the Cyperaceae comprises only two subfamilies: the Mapanioideae and the Cyperoideae. In most flowers of the Cyperoideae, the whorl of inner stamens is reduced, resulting in tetracyclic flowers. In the more primitive (scirpoid) genera within the Cyperoideae, the perianth consists of two polysymmetric whorls, whereas the perianth parts in the more derived genera have been subject to modifications and/or reduction. Comparative studies of the many silky hairs of Eriophorum and of the eight bristles of Dulichium have given rise to much discussion about their homology. Methods The spikelet and floral ontogeny in freshly collected inflorescences was investigated...
Tipo: Article / Letter to the editor Palavras-chave: Dulichium arundinaceum (L.) Britton; Eriophorum latifolium Hoppe; Floral ontogeny; Perianth; Scirpoid flower; Scirpus sylvaticus L; Scanning electron microscopy.
Ano: 2005 URL: http://www.repository.naturalis.nl/record/407290
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Gill Morphology in Two Bottom Feeder Mediterranean Sea Fishes: Grey Gurnard Fish (Eutrigla gurnardus, Linnaeus, 1758) and Striped Red Mullet Fish (Mullus barbatus surmuletus, Linnaeus, 1758) by Scanning Electron Microscopy International Journal of Morphology
Abumandour,Mohamed M. A; El-Bakary,Neveen E. R.
The purpose of the current investigation was to describe the gill morphology of two bottom feeders Mediterranean Sea fishes: Striped red mullet fish (M. surmuletus) and grey gurnard fish (E. gurnardus) that showed the same feeding habits. The gill system of the grey gurnard consisted of four pairs of gill arches while consisted of three pairs of gill arches in the striped red mullet. Our study focused on the scanning electron microscopical (SEM) features of the gills, where some differences in the gill arches and gill rakers surface in both species were observed. Our results marked that there was an interbranchial septum carrying a four transverse elevated crest in grey gurnard while in striped red mullet carrying a median longitudinal elevated crest....
Tipo: Journal article Palavras-chave: Grey gurnard fish; Striped red mullet fish; Scanning electron microscopy; Gill raker; Gill arch; Gill filament.
Ano: 2017 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022017000100014
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Leaf Surface Scanning Electron Microscopy of 16 Mulberry Genotypes (Morus spp.) with Respect to their Feeding Value in Silkworm (Bombyx mori L.) Rearing Chilean J. Agric. Res.
Singhal,B.K; Baqual,M.F; Khan,M.A; Bindroo,B.B; Dhar,A.
Mulberry (Morus spp.) is the only silkworm (Bombyx mori L.) food plant. In Indian sub tropics, S-146 is the only popular and ruling mulberry genotype for silkworm rearing. As a result, mulberry leaf availability is always the limiting factor, and therefore, sub tropics are contributing less than 1% of the country’s total silk production compared with more than 60% under tropical conditions. Besides climatic conditions, this is due to a very limited number of mulberry genotypes available in this region for silkworm rearing. However, in the mean time, 15 mulberry genotypes viz. ‘Tr-10’,‘Chinese White’,‘K-2’,‘Sujanpur...
Tipo: Journal article Palavras-chave: Scanning electron microscopy; Stomata; Trichomes; Idioblasts; Leaf yield; Sericulture.
Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392010000200001
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Micromorphology in Mycale taxonomy (Mycalidae, Poecilosclerida, Demospongiae), with the description of two new micracanthoxea-bearing species Naturalis
Carballo, José Luis; Hajdu, Eduardo.
Two new species of Mycale with micracanthoxeas are described: Mycale (Aegogropila) lilianae n. sp., mainly characterized by the presence of toxas as large as 300 µm, and Mycale (Carmia) urizae n. sp., the first Mycale with micracanthoxeas described from Africa, characterized by the presence of anisochelae-III with the falx projecting downwards from the middle basal portion of the frontal alae of the head, and by the presence of two morphologically distinct size classes of toxas, with toxas-II being notably oxea-shaped. A discussion of all species of Mycale with micracanthoxeas is included. It is postulated that a comprehensive assessment of the distribution of this character, as well as of other micromorphological features of Mycale spicules, may shed...
Tipo: Article / Letter to the editor Palavras-chave: Poecilosclerida; Mycalidae; Mycale; Taxonomy; Micromorphology; Phylogeny; Microscleres; Scanning electron microscopy.
Ano: 1998 URL: http://www.repository.naturalis.nl/record/534412
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Mineral phase in shell repair of Manila clam Venerupis philippinarum affected by brown ring disease ArchiMer
Trinkler, Nolwenn; Bardeau, Jean-francois; Marin, Frederic; Labonne, Maylis; Jolivet, Aurelie; Crassous, Philippe; Paillard, Christine.
The mineral phase of shell repair in the Manila clam Venerupis philippinarum affected by brown ring disease (BRD) was characterised at various scales and at various stages of shell repair by confocal Raman microspectrometry and scanning electron microscopy. Spherulitic and quadrangular aragonite microstructures associated with polyene pigments were clearly observed. Von Kossa staining showed that at the beginning of shell repair, hemocytes are filled with insoluble calcium carbonate salts in all fluids and then are transported toward the extrapallial fluids and the repair sites. Our analyses suggest that after a Vibrio tapetis attack and BRD deposit some clams rapidly cover the deposit, resulting in a modification in the microstructure, which could be...
Tipo: Text Palavras-chave: Aragonite; Hemocytes; Shell microstructure; Calcium carbonate; Biomineralization; Mollusc; Raman microspectrometry; Scanning electron microscopy.
Ano: 2011 URL: http://archimer.ifremer.fr/doc/00029/14065/11301.pdf
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Preservation of Haemal Nodes Fixed by Means of Two Different Aldehyde Fixatives: a Scanning Electron Microscopic Study International Journal of Morphology
Guerrero,F; Cerutti,P; Marcaccini,A.
Glutaraldehyde is the fixative most commonly used in electron microscope studies of biological tissues, however it is often necessary to use samples which were not fixed in this fixative, even with the usual uncertainty of the results that may be obtained. The fixation is the more delicate step of the sample processing. Therefore in this work, the quality of preservation of haemal nodes fixed with two classic aldehyde fixatives: formaldehyde and glutaraldehyde we have compared under the scanning electron microscope. Our results showed that both fixatives were successful in preserving the morphology of haemal nodes components; however glutaraldehyde conferred satisfactory results mainly in the preservation of parenchymal cells, whereas formaldehyde was...
Tipo: Journal article Palavras-chave: Scanning electron microscopy; Fixation; Fixative; Haemal node; Glutaraldehyde; Formaldehyde.
Ano: 2011 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022011000200059
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Recovery in culture of viable but nonculturable Vibrio parahaemolyticus: regrowth or resuscitation? ArchiMer
Coutard, Francois; Crassous, Philippe; Droguet, Michael; Gobin, Eric; Colwell, Rita; Pommepuy, Monique; Hervio Heath, Dominique.
The objective of this study was to explore the recovery of culturability of viable but nonculturable ( VBNC) Vibrio parahaemolyticus after temperature upshift and to determine whether regrowth or resuscitation occurred. A clinical strain of V. parahaemolyticus Vp5 was rendered VBNC by exposure to artificial seawater ( ASW) at 4 degrees C. Aliquots of the ASW suspension of cells ( 0.1, 1 and 10 ml) were subjected to increased temperatures of 20 degrees C and 37 degrees C. Culturability of the cells in the aliquots was monitored for colony formation on a rich medium and changes in morphology were measured by scanning ( SEM) and transmission ( TEM) electron microscopy. Samples of VBNC cells were fixed and examined by SEM, revealing a heterogeneous population...
Tipo: Text Palavras-chave: Transmission electron microscopy; Scanning electron microscopy; Resuscitation; Regrowth; Vibrio parahaemolyticus; VBNC state.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-3117.pdf
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