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Architecture and relevance of several strongly adhered biofilms over a polyester imide (PEI) surface BJM
Soncini-Júnior,Giancarlo; Franchetti,Sandra Mara Martins; Marconato,José Carlos.
A microscopical study was undertaken to analyze the eventual fungi adhesion over a polyester imide surface in enameled copper wires. Scanning electron microscopy allowed to observe in these adhered biofilms, a high amount of pigments, hyphae and its enzymatic arsenal probably acting in the surface of the polymer. Due to the highly aromatic nature of this polyester and the residual phenol derivates still present in the reticulated polymer, an antifungal activity could be expected, but no significant changes were noted in fungal growing and in the adhesion process. Additionally, the wires showed completely failure in all insulation properties. The studies aimed to understand and evaluate the great fungi potential that could be explored in biodeterioration...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fungi; Biofilms; Hyphae; Pigments; Adhesion; Scanning electron microscopy; Polymeric surface; Polyester-imide; Enameled copper wires.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500036
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Bioprotective Effect of Lactococcus piscium CNCM I-4031 Against Listeria monocytogenes Growth and Virulence ArchiMer
Saraoui, Taous; Leroi, Francoise; Chevalier, Frederique; Cappelier, Jean-michel; Passerini, Delphine; Pilet, Marie-france.
Listeria monocytogenes is a Gram-positive pathogen occurring in many refrigerated ready-to-eat foods. It is responsible for foodborne listeriosis, a rare but severe disease with a high mortality rate (20–30%). Lactococcus piscium CNCM I-4031 has the capacity to prevent the growth of L. monocytogenes in contaminated peeled and cooked shrimp and in a chemically defined medium using a cell-to-cell contact-dependent mechanism. To characterize this inhibition further, the effect of L. piscium was tested on a collection of 42 L. monocytogenes strains. All strains were inhibited but had different sensitivities. The effect of the initial concentration of the protective and the target bacteria revealed that the inhibition always occurred when L. piscium had reached...
Tipo: Text Palavras-chave: Biopreservation; Lactococcus piscium; Listeria monocytogenes; Co-culture; Cell ratio; Scanning electron microscopy; Virulence.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00449/56065/57588.pdf
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Bubaline Diaphragm Matrix: Development and Clinical Assessment into Cattle Abdominal Hernia Repair BABT
Vora,Shruti Dineshbhai; Kumar,Vineet; Asodiya,Foram Arvindbhai; Singh,Vivek Kumar; Fefar,Dhaval Tribhovanbhai; Gajera,Harsukh Popatbhai.
Abstract The purpose of the study was to develop a xenogenic bubaline diaphragm matrix (BDM) for abdominal hernia repair. A fresh diaphragm was decellularized using aqueous sodium dodecyl sulfate (SDS) solutions (0.5-4% w/v) over a period. Acellularity was confirmed histologically and characterized by Masson’s trichrome staining, scanning electron microscopy (SEM), DNA quantification, agarose gel electrophoresis, and Fourier-transform infrared spectroscopy. The BDM was used for clinical abdominal hernia repair in six cattle. Clinical, hemato-biochemical and antioxidant parameters were evaluated to assess biocompatibility of xenogenic BDM. Histologically, the diaphragm treated with 2% SDS for 48 h showed complete acellularity and orderly arranged collagen...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biocompatibility; Bubaline diaphragm matrix; DNA quantification; Spectroscopy; Scanning electron microscopy.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100309
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Characterization of neutrophil adhesion to different titanium surfaces. Repositório Alice
CAMPOS, V.; MELO, R. C. N.; SILVA, L. P. da; AQUINO, E. N.; CASTRO, M. S.; FONTES, W..
bitstream/item/179993/1/CAMPOS2014-Article-CharacterizationOfNeutrophilAd.pdf
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Titanium surfaces; Neutrophil morphology; Adhesion molecules; Inflammatory response; Flow cytometry; Scanning electron microscopy.
Ano: 2014 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/994650
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Chemical-Mineralogical Characterization of Magnetic Materials from Magnetic Soils of the Southern Espinhaço Mountain Chain and of the Upper Jequitinhonha Valley, State of Minas Gerais, Brazil Rev. Bras. Ciênc. Solo
Silva,Alexandre Christófaro; Souza,Solange de; Fabris,José Domingos; Barbosa,Maurício Soares; Barral,Uidemar Morais; Costa,Roberto Vial.
ABSTRACT In the Southern Espinhaço Mountain Chain and in the Upper Jequitinhonha Valley, magnetic soils, in different pedogenetic stages, are found to be forming over intrusions of basic lithology. The essential chemical and mineralogical properties of samples from magnetic soil profiles from those two physiographic environments in the state of Minas Gerais, Brazil, are reported. Three of the pedons (Rhodic Kandiustox – RKox, Rhodic Haplustox – RHox, and Typic Argiustoll - TAoll) were identified as being indeed developed over basic rocks; the fourth pedon (Typic Haplustox - THox) is currently forming on an acidic rock. Particle size and routine chemical analyses were performed on samples from all horizons of the four selected soil profiles. For a deeper...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Iron oxides; X ray diffractometry; Mössbauer spectroscopy; Scanning electron microscopy; Energy dispersive spectroscopy.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832017000100305
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CHORDAE TENDINEAE ARCHITECTURE IN THE PAPILLARY MUSCLE INSERTION International Journal of Morphology
Wang Gusukuma,Luciana; Prates,José Carlos; Smith,Ricardo Luiz.
Chordae tendineae architecture and their junction with papillary muscle were studied with scanning electron microscopy, comparing human and swine hearts. Papillary muscles with the corresponding chordae tendineae were obtained from human and pig (Sus scrofa) hearts after exposition of the left ventricle cavity, fixed with 4% formaldehyde. The specimens were sectioned in small samples and divided in four groups, a control one and the others submitted to digestion in a solution of 2.5% sodium hypochlorite in saline solution during 5, 10 and 15 minutes respectively. The specimens were washed and fixed in 1% osmium tetroxide in cacodylate buffer, kept in 1% tannic acid for 30 minutes, dehydrated and critical point dried, mounted and sputtered with gold. The...
Tipo: Journal article Palavras-chave: Chordae tendineae; Architecture; Human; Pigs; Scanning electron microscopy.
Ano: 2004 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022004000400005
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Citrus sinensis leaf petiole and blade colonization by Xylella fastidiosa: details of xylem vessel occlusion Scientia Agricola
Alves,Eduardo; Leite,Breno; Pascholati,Sérgio Florentino; Ishida,Maria Lúcia; Andersen,Peter Craig.
Citrus variegated chlorosis (CVC), caused by Xylella fastidiosa, is an important disease of citrus in Brazil. X. fastidiosa is restricted to xylem vessels of plants and knowledge regarding xylem colonization is still limited. Our goal was to verify how this bacterium colonizes and spreads within xylem vessels of sweet orange Citrus sinensis cv. Pêra. Petioles and pieces of leaf blades from naturally infected plant exhibiting characteristic symptoms were prepared for light microscopy (LM), scanning electron microscopy (SEM), transmission electron microscopy (TEM) and immunogold labeling (IGL). Petioles from healthy plants were used as control. IGL results, using an antibody against wall hemicelluloses, revealed that the pit membrane of vessels was altered....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ultrastructure; Scanning electron microscopy; Transmission electron microscopy; Immunolabel.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162009000200011
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Comparative leaf anatomy of some species of Abies and Picea (Pinaceae) Acta Botanica
Ghimire,Balkrishna; Lee,Chunghee; Yang,Jongcheol; Heo,Kweon.
ABSTRACTA number of conifer species are still lacking anatomical data, which is significant because morphological and anatomical data are essential for systematic study. Leaf anatomy was studied in selected species of Abiesand Piceausing light and scanning electron microscopy. Both genera were found to have typical coniferous and highly xerophytic leaves with sunken stomata and an epidermis covered by a thick cuticle. In the genus Abies, species can be differentiated by the nature of the lignified hypodermis and the number and position of resin ducts. Abies firma and A. holophylla have a continuous hypodermis whereas in A. koreana and A. nephrolepis the hypodermis is discontinuous and represented by isolated cells or groups of four or five cells. On the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Abies; Leaf anatomy; Picea; Scanning electron microscopy; Taxonomy.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062015000300346
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Comparative pollen morphological analysis in the subgenera Passiflora and Decaloba Anais da ABC (AABC)
SOARES,TALIANE L.; JESUS,ONILDO N.; SOUZA,EVERTON H.; ROSSI,MÔNICA L.; OLIVEIRA,EDER J..
ABSTRACT The genus Passiflora is the most diversified of the Passifloraceae, and its palynology presents wide morphological variability. The objective of the study was to evaluate the pollen morphology of 18 Passiflora species in order to identify informative pollinic characteristics to contribute to the taxonomic classification of the genus. The morphology of the pollen grains and the exine structure were investigated using light microscopy and scanning electron microscopy. Differences in the pollen morphology were found in the studied species, mainly in terms of shape, pollen aperture and ornamentation pattern of the exine. Most of the species belonging to the subgenus Passiflora presented ornamented 6-syncolpate pollen grains with an oblate-spheroidal...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Passifloraceae; Pollen grains; Scanning electron microscopy; Exine; Morphological characterization.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000502381
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Comparative Scanning Electron Microscopy of Platelets and Fibrin Networks of Human and Differents Animals International Journal of Morphology
Pretorius,E; Vieira,W. A; Oberholzer,H. M; Auer,R. E. J.
The purpose of the present study was to compare the platelet and fibrin network ultrastructure of humans to eight different animal species in order to determine the differences between human and animal platelet and fibrin morphology, and to determine whether the animals studied differ in their platelet and fibrin morphology, and whether these differences can be observed by scanning electron microscopy. Platelets and fibrin networks play an important role both in the coagulation process as well as physiologically in allergic processes and immunological mechanisms. The thickness of human fibrin networks were compared to mouse (Mus musculus), equine (Equus caballus), vervet monkey (Chlorocebus aethiops previously Cercopithecus aethiops), oryx (Oryx gazella),...
Tipo: Journal article Palavras-chave: Scanning electron microscopy; Platelets; Fibrin networks; Human; Animals.
Ano: 2009 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022009000100013
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Comparative Wood Anatomy of the Primuloid Clade (Ericales s.l.) Naturalis
Lens, F.; Jansen, S.; Caris, P.; Serlet, L.; Smets, E..
The wood structure of 78 species from 27 genera representing the woody primuloids (Maesaceae, Myrsinaceae, and Theophrastaceae) was investigated using light microscopy (LM) and scanning electron microscopy (SEM). Results indicated that the ray structure, the nature of mineral inclusions, and the occurrence of breakdown areas in rays can be used to separate the three primuloid families from each other. Within Ericales, the presence of exclusively multiseriate rays is synapomorphic for Myrsinaceae and Theophrastaceae, and the occurrence of breakdown areas in rays is synapomorphic for Myrsinaceae. Within Myrsinaceae, the wood structure of the mangrove genus Aegiceras differs because it has short vessel elements that are storied, non-septate fibers, a...
Tipo: Article / Letter to the editor Palavras-chave: Primuloids; Ericales s.l.; Maesaceae; Myrsinaceae; Theophrastaceae; Wood anatomy; Comparison; Light microscopy; Scanning electron microscopy.
Ano: 2005 URL: http://www.repository.naturalis.nl/record/407307
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Cytokinin preconditioning enhances multiple shoot regeneration in Pongamia pinnata (L.) Pierre - a potential, non-edible tree seed oil source for biodiesel Electron. J. Biotechnol.
Belide,Srinivas; Sajjalaguddam,Ramgopal Rao; Paladugu,Anitha.
An efficient, highly reproducible protocol for multiple shoot induction and plant regeneration of Pongamia pinnata has been successfully developed using cotyledonary node explants. This study also demonstrates that preconditioning of explant stimulates production of multiple shoots from cotyledonary nodes of P. pinnata. The highest direct shoot regeneration (90%) with an average of 18.4 � 3.1 shoots/explant were obtained when cotyledonary node explants were excised from seedlings germinated on Murashige and Skoog (MS) media supplemented with benzyladenine (BA) 1 mg l-1, and subsequently cultured on MS media with 1 mgl-1 thidiazuron (TDZ). Scanning electron microscope observations of cotyledonary node (CN) explants excised from pre-conditioned and normal...
Tipo: Journal article Palavras-chave: Benzyladenine; Cotyledonary node; Multiple shoot induction; Scanning electron microscopy; Thidiazuron.
Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000600003
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Early somatic embryogenesis in Heliconia chartacea Lane ex Barreiros cv. Sexy Pink ovary section explants BABT
Ulisses,Cláudia; Camara,Terezinha Rangel; Willadino,Lilia; Albuquerque,Cynthia Cavalcanti de; Brito,Júlio Zoé de.
The present work evaluated the development of embryogenic callus from transversal ovary sections. The experiments were carried out under two experimental regimes using combinations of IAA (0; 5.71; 8.56; 11.42; 14.27μM) and 2,4-D (0; 13.57; 18.10; 22.62μM) or combinations of 2,4-D with BA (0; 4.43; 6.65; 8.87; 11.09μM). Assessments were made of anatomical aspects of the callus and for the presence of embryogenic structures using cytochemical and histological analyses and stereomicroscopic and scanning electronic microscopic observations. Treatments with 2,4-D and IAA produced friable calluses demonstrating cellular acquisition of morphogenetic competence as well as the formation of pro-embryogenic sectors. The expression of embryogenic program could be...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Callogenesis; Growth regulator; Histology; Scanning electron microscopy.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000100002
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Ecophysiological, anatomical and ultrastructural characteristics of Vitex polygama Cham. (Verbenaceae) submitted to different concentrations of fluoride Biological Sciences
Rossi, Zélia Terezinha Teixeira; Castro, Evaristo Mauro de; Pereira, Giuslan Carvalho; Barbosa, João Paulo Rodrigues Alves Delfino; Carvalho, Graziele Wolff de Almeida; Rossi, Marco Aurélio de Lima Silva; Pereira, Fabrício José.
Fluorides released during the manufacture of aluminum, fertilizers, glass and ceramics are considered to be highly toxic to plants, causing damages in relatively small concentrations. This study aimed to evaluate fluoride (F) accumulation and its effects on Vitex polygama Cham. (Verbenaceae) exposed to simulated rain containing potassium fluoride in concentrations of 5, 10, 15 and 20 mg L-1. Vitex polygama presented no visual symptoms of foliar injury and accumulated a relatively small amount of F-, significant only for the treatments with higher concentrations of the pollutant.  F- promoted an increase in non-photochemical quenching (qN) and in the coefficient due to non-photochemical extinction (NPQ), which indicates higher dissipation of radiant energy...
Tipo: Info:eu-repo/semantics/article Palavras-chave: 2.03.00.00-0; 2.03.02.03-7; 2.03.03.00-9 chlorophyll a fluorescence; Potassium fluoride; Scanning electron microscopy; Transmission electron microscopy; Biomonitoring studies Botânica; Anatomia Vegetal; Fisiologia Vegetal.
Ano: 2016 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/31400
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Effect of convective and microwave methods on drying characteristics, color, rehydration and microstructure properties of ginger Ciênc. Tecnol. Aliment.
IZLI,Nazmi; POLAT,Ahmet.
Abstract In this research, the effects of convective drying method (60, 70 and 80 °C) and microwave drying method (160 and 350 W) on the drying characteristics, color, rehydration and microstructure properties of ginger has been examined. For the purpose of choosing the most appropriate thin-layer drying models in terms of the drying applications, nine mathematical models have been applied on the experimental data. Pursuant to the assessment of the statistical tests, models of Diffusion Approach, Midilli et al. and Page that represent drying characteristics are found to be better than other models. Drying applications have affected the color values of all fresh ginger samples. Results indicated that browning degree increases as drying temperature and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Color; Drying characteristics; Ginger; Rehydration ratio; Scanning electron microscopy.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612019000300652
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Effect of fungicide on Fusarium verticillioidesmycelial morphology and fumonisin B1 production BJM
Miguel,Tatiana de Á.; Bordini,Jaqueline G.; Saito,Gervásio H.; Andrade,Célia G.T. de J.; Ono,Mario A.; Hirooka,Elisa Y.; Vizoni,Édio; Ono,Elisabete Y.S..
The effect of fludioxonil + metalaxyl-M on the mycelial morphology, sporulation and fumonisin B1 production by Fusarium verticillioides 103 F was evaluated. Scanning electron microscopy analysis showed that the fungicide caused inhibition of hyphal growth and defects on hyphae morphology such as cell wall disruption, withered hyphae, and excessive septation. In addition, extracellular material around the hyphae was rarely observed in the presence of fludioxonil + metalaxyl-M. While promoting the reduction of mycelial growth, the fungicide increased sporulation of F. verticillioides compared to the control, and the highest production occurred on the 14th day in the treatments and on the 10th day in the control cultures. Fumonisin B1production in the culture...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Toxigenic fungi; Mycotoxin; Scanning electron microscopy; Electron micrographs; Extracellular material.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000100293
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Effect of morphological heterogeneity of somatic embryos of Melia azedarach on conversion into plants Biocell
Vila,Silvia; Gonzalez,Ana; Rey,Hebe; Mroginski,Luis.
Embryogenic cultures were initiated from immature Melia azedarach (Meliaceae) zigotic embryos. Explants were induced on Murashige and Skoog (1962) medium with 4.54 μM thidiazuron or 0.45 μM dichlorophenoxyacetic acid. After 6 weeks of culture on induction medium, somatic embryos were categorized in four morphological classes based on the presence of single or fused embryos and if they remained united or not to the original explant; that were evaluated histologically. The somatic embryos of every category were transferred, in groups or individually, on a 1/4 MS medium. Bipolar embryos, the more typically normal ones, had well defined shoot and root apical meristems and produced single plants; subcultured individually their conversion was...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Somatic embryo morphology; Conversion; Histology; Scanning electron microscopy.
Ano: 2010 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452010000100002
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Effect of ultrasonication of Switchgrass on fermentable sugar production and biomass physical structure CIGR Journal
Sharma, Rajat; Sharma-Shivappa, Ratna R.; Boyette, Michael D.; Stikeleather, Larry F..
Pretreatment of lignocellulosic biomass for enhancing sugar yields has been studied extensively over the years.  Conventional methods employed to preprocess biomass to make it conducive for enzymatic hydrolysis have been hampered by issues like poor energy efficiency and production of undesirable by-products.  Ultrasonication, which involves the treatment of biomass through ultrasonic sound waves in a liquid medium without additional chemicals, is believed to have potential for biomass pretreatment.  In this study the effects of ultrasonication on switchgrass, a potential feedstock for bioethanol production due to its high cellulosic content, were investigated.  Results of compositional analysis and scanning electron microscopy conducted to visualize...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Panicum virgatum; Amplitude; Pretreatment; Hydrolysis; Scanning electron microscopy.
Ano: 2013 URL: http://www.cigrjournal.org/index.php/Ejounral/article/view/2549
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Essential oils and whole milk in the control of soybean powdery mildew Ciência Rural
Perina,Fabiano José; Alves,Eduardo; Pereira,Ricardo Borges; Lucas,Gilvaine Ciavareli; Labory,Claudia Regina Gontijo; Castro,Hilário Antonio de.
This research aimed to evaluate the potential of essential oils (EOs) and cow's whole milk (CWM) in order to control soybean powdery mildew and to estimate the most effective concentrations of these natural products in reducing the disease severity on soybean plants. Three experiments were carried out: The first experiment evaluated and selected the most effective treatments to reduce the severity of soybean powdery mildew under greenhouse conditions; the second experiment evaluated the effect of CWM and EOs of citronella, lemongrass, eucalyptus, cinnamon and tea tree on the pathogen through the ultrastructure analysis of soybean leaflets infected by Erysiphe diffusa using the scanning electron microscope (SEM) and light microscope (LM) technology. In the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Erysiphe diffusa; Glycine max; Essential oil as biocontrol; Scanning electron microscopy; Effective concentrations.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782013001100003
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Etude du biofilm microalgal des vasières intertidales : dynamique spatio-temporelle à micro-échelle et performances photosynthétiques ArchiMer
Herlory, Olivier.
The microalgal biofilm, which is present on sediment surface, is the primary production unit on bare intertidal mudlfats. In order to improve our understanding of this primary production system, a study was undertaken at microscale to analyse the structural and functional properties of this biofilm. In the first part of this work, a detailed descriptive analysis lead to the characterisation of the spatiotemporal dynamics of the physical structure of the biofilm. It turns out that the biofilm exhibits its own dynamics at the sediment surface (as a discrete compartment in the top 200 µm), but still has a functional relationship with the total available biomass (PCB) distributed within the top 1 cm (linear relationship between the maximum size of the biofilm...
Tipo: Text Palavras-chave: PAM Fluorometry; Scanning electron microscopy; Photosynthesis; Spatio temporal dynamic; Micro scale; Microphytobenthic biofilm; Intertidal mudflats; Fluorimétrie PAM; Microscopie électronique à balayage; Photosynthèse; Dynamique spatio temporelle; Micro échelle; Biofilm microphytobenthique; Vasière intertidal.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/these-2138.pdf
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