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ISSR as new markers for genetic characterization and evaluation of relationships among phytoplankton ArchiMer
Bornet, Benjamin; Antoine, Elisabeth; Bardouil, Michele; Marcaillou-le Baut, Claire.
In order to increase the molecular tools and markers needed for the identification of phytoplankton species, the inter simple sequence repeat ( ISSR) fingerprinting was adapted to micro-algae and its use in genetic analysis was demonstrated. Twelve strains, 6 Alexandrium, 4 Pseudo-nitzschia, 1 Skeletonema and 1 Tetraselmis were analysed for the first time with ISSR amplifications. The patterns were highly polymorphic and very reproducible. The 6 primers gave 223 polymorphic markers that clearly and easily distinguished all 12 strains ( mainly toxic ones) and gave 187 polymorphic markers among the Alexandrium and the Pseudo-nitzschia species. ISSR amplifications also indicated a large occurrence of simple sequence repeat ( SSR) in phytoplankton genomes,...
Tipo: Text Palavras-chave: Tetraselmis; Skeletonema; Pseudo nitzschia; ISSR; Genetic characterization and diversity; Fingerprints; Alexandrium.
Ano: 2004 URL: http://archimer.ifremer.fr/doc/2004/publication-516.pdf
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Large-scale microalgae production for nursery rearing of marine bivalves OMA
De Pauw, N.; Verboven, J.; Claus, C..
The feasibility of large-scale bloom induction of nutritionally suited natural phytoplankton species, to feed a semi-industrial nursery of edible shellfish, built on the Belgian coast, was tested. The outdoor microalgal production unit consisted of four tanks of 100 m² surface each (two of 1 m depth and two of 0.5 m depth), equipped with different mixing devices. The cultures were run as chemostats in which seawater was enriched with commercial inorganic N, P and Si fertilizers. Depending on the season, between 5-10 and 80% of the culture volume could be harvested daily, with algal densities ranging from 50,000 to 500,000 cells per ml. By manipulation of operational parameters such as detention time, nutrient levels and nutrient ratios (N: Si: P), unsuited...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Algal culture; Marine aquaculture; Mass culture; Mollusc culture; Rearing; Chaetoceros; Chlorella; Nitzchia; Phaeodactylum; Skeletonema.
Ano: 1983 URL: http://www.vliz.be/nl/open-marien-archief?module=ref&refid=3395
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