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Addition of benzyladenine to coconut explant cultured In vitro improves the formation of somatic embryos and their germination Agrociencia
Montero-Cortés,Mayra I.; Chan-Rodríguez,José L.; Cordova-Lara,Ivan; Oropeza-Salin,Carlos; Sáenz-Carbonell,Luis.
Coconut (Cocos nucífera L.) production has been declining in México and other countries due to deadly diseases; therefore, massive production of palms resistant to diseases is needed for replanting. Production of somatic embryos and micropropagation could be a very useful technique to produce free disease coconut population. Explants (embryogenic-structures) of Pacific Tall 2 ecotype were cultured in an induction medium defined as I (semisolid Y3 medium containing 0.65 mM 2,4-dichlorophenoxyacetic acid) and treated with the cytokinin benzyladenine (BA) at 0, 25, 100 or 200 ,µM to evaluate its effect on embryogenic callus formation. The BA treatments did not affect the percentage of embryogenic callus formation. In order to evaluate the effect of the BA...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cocos nucífera; Clonal propagation; Somatic embryogenesis; Cytokinin.
Ano: 2011 URL: http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S1405-31952011000600002
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AgNO3: a potential regulator of ethylene activity and plant growth modulator Electron. J. Biotechnol.
Kumar,Vinod; Parvatam,Giridhar; Ravishankar,Gokare Aswathanarayana.
The aim of this review is to critically analyze the role of silver nitrate (AgNO3) in modulating plant growth and development. In recent years, basic studies on ethylene regulation opened new vistas for applied research in the area of micro-propagation, somatic embryogenesis, in vitro flowering, growth promotion, fruit ripening, and sex expression. Silver nitrate has proved to be a very potent inhibitor of ethylene action and is widely used in plant tissue culture. Few properties of silver nitrate such as easy availability, solubility in water, specificity and stability make it very useful for various applications in exploiting plant growth regulation and morphogenesis in vivo and in vitro. Silver ion mediated responses seem to be involved in polyamines,...
Tipo: Journal article Palavras-chave: Calcium; Ethylene; Morphogenesis; Polyamines; Silver nitrate; Somatic embryogenesis.
Ano: 2009 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582009000200008
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Cell suspension culture and plant regeneration of a Brazilian plantain, cultivar Terra. Repositório Alice
MORAIS-LINO, L.S.; SANTOS-SEREJO, J.A. dos; SILVA, S. de O. e; SANTANA, J.R.F. de; KOBAYASHI, A.K..
The objective of this study was to establish cell suspension culture and plant regeneration via somatic embryogenesis of a Brazilian plantain, cultivar Terra Maranhão, AAB. Immature male flowers were used as explant source for generating highly embryogenic cultures 45 days after inoculation, which were used for establishment of cell suspension culture and multiplication of secondary somatic embryos. Five semisolid culture media were tested for differentiation, maturation, somatic embryos germination and for plant regeneration. An average of 558 plants per one milliliter of 5% SCV (settled cell volume) were regenerated in the MS medium, with 11.4 µM indolacetic acid and 2.2 µM 6-benzylaminopurine. Regenerated plants showed a normal development, and no...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Musa; Culture media; Propagation; Somatic embryogenesis; Meio de cultura; Micropropagação; Embriogênese somática.
Ano: 2008 URL: http://www.alice.cnptia.embrapa.br/handle/doc/122219
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Comparison of Economic Efficiency between in Vitro and Field Methods for Vegetative Propagation of Coffea Canephora. Repositório Alice
SANTOS, M. R. A. dos; SOUZA, C. A. de; ROCHA, J. F. da; ARAUJO, L. V. de; ESPINDULA, M. C..
The objective of this study was to approach the viability of the two forms of propagation, comparing cost and time for the production of new plantlets and number of plantlets produced in each propagation system..
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Coffee; Somatic embryogenesis; Rooting of cuttings; Production cost; Café conilon; Embriogênese somática; Enraizamento de estacas; Custo de produção.
Ano: 2015 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1020428
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Direct somatic embryogenesis and organogenesis pathway of plant regeneration can seldom occur simultaneously within the same explant of sorghum Open Agri
Girijashankar, V..
Palavras-chave: Somatic embryos; Transgenics; Somatic embryogenesis; Auxins; Regeneration; Protocols; Sorghum; Cytokinins; Embryonic development; Transgenic plants.
Ano: 2007 URL: http://agropedia.iitk.ac.in/openaccess/?q=node/3300
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Efeitos do metano sulfonato de etila sobre a sobrevivência e capacidade de regeneração de embriões somáticos de bananeira. Repositório Alice
MOREIRA, C. V.; SILVA, S. de O. e; SEREJO, J. A. dos S.; LINO, L. S. M.; NEPOMUCENO, C. F..
O presente estudo tem como objetivo avaliar a sensibilidade e o desenvolvimento de embriões somáticos de bananeira ?Tropical? submetidos ao tratamento com Metano Sulfonato de Etila (EMS) como agente mutagênico.
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Embriogênese somática; Musa spp; Banana; Mutação somática; Somatic mutation; Somatic embryogenesis.
Ano: 2014 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1006063
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Encapsulated Somatic Embryos and Zygotic Embryos for Obtaining Artificial Seeds of Rauli-Beech (Nothofagus alpina (Poepp. & Endl.) Oerst.) Chilean J. Agric. Res.
Cartes R,Priscila; Castellanos B,Hermes; Ríos L,Darcy; Sáez C,Katia; Spierccolli H,Scarlette; Sánchez O,Manuel.
Somatic and zygotic embryos from mature seeds of rauli-beech, Nothofagus alpina (Poepp. & Endl.) Oerst., were encapsulated in different artificial endosperms in order to generate a cover that fulfills the function of nourishment and protection of the embryos, facilitating their later germination. The content of sodium alginate varied by 4%, 3%, and 2%, as did the immersion time in calcium chloride (CaCl2), which acts as complexing agent. The artificial endosperm components of the Murashige and Skoog medium (MS) were added, supplemented with 0.5 mg L-1 indolacetic acid (IAA), 0.5 mg L-1 naphthaleneacetic acid (NAA), 2 mg L-1 6-benzylaminopurine (BAP) and 30 g L-1 sucrose. The germinative behaviors of encapsulated somatic and zygotic embryos were...
Tipo: Journal article Palavras-chave: Rauli-beech; Somatic embryogenesis; Synthetic seed; Sodium alginate.
Ano: 2009 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392009000100014
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Endogenous Quantification of Abscisic Acid and Indole-3-Acetic Acid in Somatic and Zigotic Embryos of Nothofagus alpina (Poepp. & Endl.) Oerst Chilean J. Agric. Res.
Cartes Riquelme,Pricila; Ríos Leal,Darcy; Sáez Carrillo,Kátia; Uribe Moraga,Matilde; Valenzuela Aguilar,Sofía; Joseph Bolus,Stephen; Sánchez Olate,Manuel.
Abscisic acid (ABA) and indole-3-acetic acid (IAA) participate in the propagation of plants by somatic embryogenesis, causing polar structural differentiation of the embryo. The goal of the assay was to compare endogenous levels of ABA and IAA between somatic embryos (SE) and zygotic embryos (ZE) of Nothofagus alpina (Poepp. & Endl.) Oerst. In this study, a somatic embryo maturation assay involving the addition of varying concentrations of exogenous ABA was performed on cotyledonary-stage of N. alpina. Furthermore, the endogenous levels of ABA and IAA were quantified in the immature ZE, the mature ZE, and the embryonic axis of a mature embryo of N. alpina. The current study utilized high performance liquid chromatography (HPLC) for quantification....
Tipo: Journal article Palavras-chave: Somatic embryogenesis; Maturation; Germination; HPLC.
Ano: 2011 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392011000400007
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Evaluation of soybean cultivars on the embryogenic and organogenic potential - doi: 10.4025/actasciagron.v33i1.12344 Agronomy
Texeira, Leandra Regina; UEM; Braccini, Alessandro De Lucca e; UEM; Churata, Bruno Grimaldo Martinho; UFPR; Vieira, Elisa Serra Negra; Cooperativa Central de Pesquisa Agrícola; Martins, Polyana Kelly; Cooperativa Central de Pesquisa Agrícola; Schuster, Ivan; Cooperativa Central de Pesquisa Agrícola.
The first step in plant transformation using somatic embryogenesis or organogenesis for plant regeneration in vitro has been the identification of genotypes with the best totipotency. Thus, the objective of this experiment was to evaluate the totipotency of ten cultivars and one soybean line from the Coodetec Gene Bank. The somatic embryos developed from immature cotyledons measuring from 4 to 6 mm in length, and the organogenesis was evaluated from mature soybean seeds. The cultivars CD 201, CD 216 and CD 220 were the most promising genotypes to produce somatic embryos, whereas the cultivar CD 217 had the highest quantity of differentiated explants, which represents the best regeneration efficiency for participating in organogenic systems. In the...
Tipo: Laboratory analysis Palavras-chave: Glycine max; Somatic embryogenesis; Organogenesis Glycine max; Somatic embryogenesis; Organogenesis Melhoramento Vegetal; Cultura de Tecidos.
Ano: 2011 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/12344
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Expression of RNA polymerase IV and V in Oryza sativa Electron. J. Biotechnol.
Moo,Leydi de Rocio Canche; González,Angela Kú; Rodríguez-Zapata,Luis Carlos; Suarez,Victor; Castaño,Enrique.
RNA polymerase IV and V are principal players in the RdDM pathway, where their current study has shown interaction of several factors that control DNA silencing of intergenic regions and siRNA production. DNA silencing is an important process during cell differentiation, nuclear structure and viral control. However, RNA pol IV and V are yet to be study in model monocot systems like Oryza sativa that can provide further information on genetic silence mechanism in plats. We show the expression pattern of these polymerases in tissues extracts of Oryza sativa. Detectable amounts of these polymerases are found in specific adult plant tissues and particularly expressed during somatic embryogenesis but not during early stages of normal embryo development. The use...
Tipo: Journal article Palavras-chave: Oryza sativa; RNA pol IV; RNA pol V; Somatic embryogenesis; Zygotic embryogenesis.
Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000200009
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Formação de calos embriogênicos em genótipos de milho tropical. Repositório Alice
SALGADO, F. F.; CARNEIRO, A. A.; ALVES, M. de C..
2016
Tipo: Artigo em anais de congresso (ALICE) Palavras-chave: Embriogênese somática; Embriogênese; Embryogenesis; Somatic embryogenesis.
Ano: 2016 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1054866
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Histocytological examination on organogenesis and somatic embryogenesis of HBsAg-transgenic cherry tomato mutant Phyton
Guan,Z-J; Guo,B; Huo,Y-L; Dai,J-K; Wei,Y-H.
The initiation and development of organogenic buds and somatic embryos in HBsAg-transgenic cherry tomato (Lycopersicum esculentum var. cerasiforme) mutant were studied histologically. The leaf explants of the mutant were cultured on Murashige & Skoog (MS) basal medium supplemented with 6-BA 1.0 mg/L and IAA 0.05 mg/L for callus induction. Histological studies on the leaf explants of the mutant at various developmental stages revealed that organogenic buds first appeared in the axillary position of explants on the 14th cultured day, and then somatic embryos formed in the same mutant explants after 35 days of culture. Transmission electron microscopy and scanning electron microscopy indicated that there were significant changes in morphology and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Histocytological; Organogenesis; Somatic embryogenesis; HBsAg; Transgenic; Cherry tomato; Mutant.
Ano: 2012 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-56572012000100008
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In vitro callogenesis in anthers of Hevea spp. Repositório Alice
SILVA, E. J. D. da; CORDEIRO, E. R.; QUISEN, R. C..
bitstream/item/191921/1/2018-RAC-ReginaQ-IUFRO-Coimbra-In-vitro.pdf
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Rubber tree; Haploid culture; Plant growth regulators; Hevea; Seringueira; Somatic embryogenesis.
Ano: 2018 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1104940
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Induction of somatic embryogenesis and plant regeneration of sugarcane cv. RB925345 from somatic embryos Sci. Agrar.Paran. / SAP
Almeida, Rodrigo de Oliveira; Silva, Sérgio Delmar dos Anjos e; Dutra, Leonardo Ferreira.
The aim of this study was to establish a protocol for induction of somatic embryogenesis and regeneration of somatic embryos of sugarcane (cultivar RB925345). Best results was obtained using the concentration of 13.5 µM L-1 2,4-dichlorophenoxyacetic acid, 45 days of incubation in induction medium, one subculture and 15 days of incubation in regeneration medium, providing 47.5% of embryogenic calli and 38.9% of plants regenerated. Adjust are necessary in this protocol to increase the rate of embryogenic calli production and regeneration of somatic embryos.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Saccharum spp.; 2 4-dichlorophenoxyacetic acid; Plant tissue culture; Somatic embryogenesis.
Ano: 2018 URL: http://e-revista.unioeste.br/index.php/scientiaagraria/article/view/14165
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Induction of somatic embryogenesis as an example of stress-related plant reactions Electron. J. Biotechnol.
Zavattieri,Maria Amely; Frederico,António Miguel; Lima,Mónica; Sabino,Rui; Arnholdt-Schmitt,Birgit.
In this review, we address the role of stress as one of the principal causes for a cell or tissue to change its pre-existing somatic program, reprogramming itself to express the embryogenic pathway. The focus of this paper is the effect of different stress conditions on the induction phase of plant somatic embryogenesis, as well as the development of embryogenic competence as a result of the applied stresses. We also present a variety of data that link plant somatic embryogenesis, DNA methylation and oxidative stress response.
Tipo: Journal article Palavras-chave: Cell reprogramming; Reactive oxygen species; Somatic embryogenesis; Stress.
Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000100012
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Influence of 2,4-D and BAP on callus growth and the subsequent regeneration of somatic embryos in long-term cultures of Pelargonium x domesticum cv. Madame Layal Electron. J. Biotechnol.
Haensch,Klaus-Thomas.
This long-term study demonstrates for the first time that it is possible to propagate embryogenic cultures in pelargoniums and to subsequently initiate the differentiation of embryos using the cultivar Madame Layal (Pelargonium x domesticum). Propagation of callus was only possible with combinations of 2,4-dichlorophenoxyacetic acid (2,4-D) and 6-benzylaminopurine (BAP), which gave rise to embryos from the primary culture stage on. However, the propagation of cells, as well as the differentiation of embryos, was inhibited by a continuous application of these growth regulators. For this reason, a long period on medium lacking growth regulators was necessary before the differentiation of embryos occurred again. The consequences for improving the propagation...
Tipo: Journal article Palavras-chave: Auxin; Cytokinin; Propagation; Somatic embryogenesis.
Ano: 2007 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000100007
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Meios alternativos para o cultivo in vitro de calos embriogênicos das tangerineiras Sunki Tropical e Cleópatra. Repositório Alice
SANTOS, E. B.; SOUZA, A. da S.; CARVALHO, M. de J. da S. de; SOARES FILHO, W. dos S..
O desenvolvimento de novas biotecnologias, associadas aos programas convencionais de melhoramento, poderão resultar na produção de novas variedades de citros. Diversas técnicas de cultura de tecidos têm sido desenvolvidas em citros, visando, entre outras aplicações, facilitar a manipulação genética, de maneira a superar as limitações encontradas no melhoramento convencional e efetivamente contribuir na geração de novos genótipos.
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Tangerina; Citrus; Somatic embryogenesis; Breending.
Ano: 2013 URL: http://www.alice.cnptia.embrapa.br/handle/doc/965612
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Micropropagação de bambu em largaescala: princípios, estratégias e desafios. Repositório Alice
NOGUEIRA, J. S.; COSTA, F. H. da S.; VALE, P. A. A. do; LUIS, Z. G.; SCHERWINSKI-PEREIRA, J. E..
bitstream/item/170604/1/26392-105-131.pdf
Tipo: Capítulo em livro científico (ALICE) Palavras-chave: Poaceae; Bambusoideae; Embriogênese somática; Biorreatores de imersão temporária; Meio líquido; Micropropagação de baixo custo; Temporary immersion systems; Liquid medium; Low-cost micropropagation; Poaceae; Somatic embryogenesis.
Ano: 2017 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1084622
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Physiological and biochemical changes during organogenesis and somatic embryogenesis of HBsAg-transgenic cherry tomato mutant Phyton
Guan,Z-J; Lu,S-B; Huo,Y-L; Guo,B; Guan,Z-P; Wei,Y-H.
Leaf explants of the HBsAg-transgenic cherry tomato (Solanum lycopersicum) mutant were cultured on Murashige and Skoog (MS) basal medium, supplemented with 1.0 mg/L 6-BA and 0.05 mg/L IAA for callus induction, to clarify the physiological and biochemical characteristics of morphogenesis development. Therefore, the physiological and biochemical changes during the development of organogenic shoots and somatic embryos in the mutant were studied. Superoxide dismutase (SOD) activities of the mutant had only one peak value on the 21st day. Peroxidase (POD) activities of the mutant declined less sharply since the explants were cultured. IAA oxidase activity of the mutant increased steadily until 42 days from culturing and then decreased sharply. Malondialdehyde...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Physiological; Biochemical; Organogenesis; Somatic embryogenesis; HBsAg-transgenic; Cherry tomato; Mutant.
Ano: 2015 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-56572015000200013
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Plant regeneration via indirect somatic embryogenesis and optimisation of genetic transformation in Coffea arabica L. cvs. Caturra and Catuaí Electron. J. Biotechnol.
Gatica-Arias,Andrés M; Arrieta-Espinoza,Griselda; Espinoza Esquivel,Ana M.
A protocol for Coffea arabica L. cvs. Caturra and Catuaí plant regeneration via indirect somatic embryogenesis (ISE) was established. Furthermore, a biolistic mediated genetic transformation protocol was optimized for Catuaí callus aggregates. Maximum callus induction was obtained when Caturra (87%) and Catuaí (67%) leaves were cultured on Murashige and Skoog medium with 18.56 µM kinetin and 4.52 µM2,4-dichlorophenoxyacetic acid (2,4-D). Catuaí suspension cultures were established from embryogenic callus using liquid proliferation CP and Sli media and diffused light and darkness. The higher suspension cultures fresh weight was obtained using Erlenmeyer (1425.4 ± 354.9 mg) than Recipient for Automated Temporary Immersion System (RITA®) (518.6 ± 55.1 mg),...
Tipo: Journal article Palavras-chave: Bioreactor; Coffee; Genetic transformation; Somatic embryogenesis; Suspension cultures.
Ano: 2008 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000100010
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