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Addition of benzyladenine to coconut explant cultured In vitro improves the formation of somatic embryos and their germination Agrociencia
Montero-Cortés,Mayra I.; Chan-Rodríguez,José L.; Cordova-Lara,Ivan; Oropeza-Salin,Carlos; Sáenz-Carbonell,Luis.
Coconut (Cocos nucífera L.) production has been declining in México and other countries due to deadly diseases; therefore, massive production of palms resistant to diseases is needed for replanting. Production of somatic embryos and micropropagation could be a very useful technique to produce free disease coconut population. Explants (embryogenic-structures) of Pacific Tall 2 ecotype were cultured in an induction medium defined as I (semisolid Y3 medium containing 0.65 mM 2,4-dichlorophenoxyacetic acid) and treated with the cytokinin benzyladenine (BA) at 0, 25, 100 or 200 ,µM to evaluate its effect on embryogenic callus formation. The BA treatments did not affect the percentage of embryogenic callus formation. In order to evaluate the effect of the BA...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cocos nucífera; Clonal propagation; Somatic embryogenesis; Cytokinin.
Ano: 2011 URL: http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S1405-31952011000600002
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Advances observed in papaya tree propagation Rev. Bras. Frutic.
Costa,Adelaide de Fátima Santana da; Abreu,Emanuel Felipe Medeiros; Schmildt,Edilson Romais; Costa,Aureliano Nogueira da; Schmildt,Omar.
Abstract Papaya (Carica papaya L.) is a fruit tree of great economic and food interest worldwide. Its propagation can occur both sexually and asexually. Traditionally, it is propagated by seeds; however, vegetative propagation has been often used with the use of in vitro and ex vitro culture techniques. In vegetative propagation, the cutting method has been extensively explored. Research has also been developed using grafting. In recent years, papaya cultivation has also become a model for other fruit plants in the use of biotechnological techniques aiming applications ranging from germplasm conservation to genetic transformation of plants. Since the beginning of the 1970s, the culture of papaya tree tissues has been intensely studied, allowing greater...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Carica papaya L.; Propagation of papaya; Vegetative propagation; Somatic embryogenesis.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452019000501001
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AgNO3: a potential regulator of ethylene activity and plant growth modulator Electron. J. Biotechnol.
Kumar,Vinod; Parvatam,Giridhar; Ravishankar,Gokare Aswathanarayana.
The aim of this review is to critically analyze the role of silver nitrate (AgNO3) in modulating plant growth and development. In recent years, basic studies on ethylene regulation opened new vistas for applied research in the area of micro-propagation, somatic embryogenesis, in vitro flowering, growth promotion, fruit ripening, and sex expression. Silver nitrate has proved to be a very potent inhibitor of ethylene action and is widely used in plant tissue culture. Few properties of silver nitrate such as easy availability, solubility in water, specificity and stability make it very useful for various applications in exploiting plant growth regulation and morphogenesis in vivo and in vitro. Silver ion mediated responses seem to be involved in polyamines,...
Tipo: Journal article Palavras-chave: Calcium; Ethylene; Morphogenesis; Polyamines; Silver nitrate; Somatic embryogenesis.
Ano: 2009 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582009000200008
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Anatomy of somatic embryogenesis in Carica papaya L. BABT
Fernando,Juliana A.; Melo,Murilo; Soares,Marli K. M.; Appezzato-da-Glória,Beatriz.
Mature zygotic embryos of Carica papaya L. ‘Sunrise Solo’ were used as explants for embryogenesis induction. The explants were inoculated on Murashige and Skoog culture medium supplemented with 2 mg.L-1 2,4-dichlorophenoxyacetic acid and incubated in darkness at 25+2°C. Histological analysis of callogenesis and somatic embryogenesis indicated occurrence of direct and indirect somatic embryogenesis development. Direct somatic embryo formation was observed from hypocotyledonary epidermic cells only from explant 18 days after inoculation. Somatic embryos formed indirectly were originated from embryogenic superficial cells of pre-embryonic complexes located on peripherical and on internal cell layers of callus 49 days after inoculation. Diverse morphological...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Anatomy; Somatic embryogenesis; Papaya.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132001000300005
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Assessment of somaclonal variation in indirect morphogenesis-derived plants of Arracacia xanthorrhiza PAB
Vitamvas,Jan; Viehmannova,Iva; Cepkova,Petra Hlasna; Mrhalova,Hana; Eliasova,Katerina.
Abstract: The objective of this work was to induce and detect somaclonal variation in arracacha (Arracacia xanthorrhiza) plants regenerated via indirect morphogenesis, in order to evaluate the potential of this technique to produce new genotypes for breeding purposes of this crop. Calli were induced from petiole segments on Murashige & Skoog (MS) medium supplied with 0.1 mg L-1 2,4-dichlorophenoxyacetic acid. The regeneration of plants via indirect morphogenesis was carried out on half-strength MS medium without plant growth regulators. Fifteen randomly chosen plants were subjected to flow cytometry and “inter-simple sequence repeat” (ISSR) analysis. Ploidy level remained stable in all tested regenerants (2n=4x=44), with no changes in the genome....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Arracacha; ISSR; Plant breeding; Ploidy level; Somatic embryogenesis.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2019000102200
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Biochemical and morphological changes during the growth kinetics of Araucaria angustifolia suspension cultures BABT
Santos,André Luis Wendt dos; Silveira,Vanildo; Steiner,Neusa; Maraschin,Marcelo; Guerra,Miguel Pedro.
Embryogenic cultures of Araucaria angustifolia were established in a BM liquid medium supplemented with 2 µM 2,4-dichlorophenoxyacetic acid, 1 µM 6-benzylaminopurine and 1 µM kinetin (BM2) and in a BM medium free of growth regulators (BM0). During 42 days in culture, the cell growth pattern of both cultures was similar. The pH of the culture medium of both BM0 and BM2 underwent progressive reduction during culture time. For both the embryogenic cultures a preferential uptake of glucose in the late stages of cell growth kinetics was observed. The extracellular protein content was similar for both the embryogenic cultures. Acetocarmine and Evan's blue double stain showed major differences for early somatic embryo organisation, in which only the embryogenic...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Araucariaceae; Glucose; PH changes; Protein; Somatic embryogenesis.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000300001
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Bioreactors in coffee micropropagation Braz. J. Plant Physiol.
Etienne,Hervé; Dechamp,E; Barry-Etienne,D; Bertrand,Bernóit.
In coffee, bioreactors are the most promising way for scaling-up micropropagation processes, particularly somatic embryogenesis. The availability of an efficient somatic embryogenesis process would allow the rapid mass production of heterozygous materials such as selected Coffea canephora clones and F1 Arabica hybrid varieties. For the last fifteen years, bioreactors (mechanically or pneumatically agitated bioreactors, temporary immersion bioreactors) have mostly been used on coffee to optimize the mass regeneration of somatic embryos from embryogenic tissues. This review presents the main results, obtained with several bioreactor models, concerning the different steps of the micropropagation process : i) the multiplication of embryogenic tissues, ii) the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Coffea; Breeding; Liquid medium; Mass propagation; Somatic embryogenesis; Temporary immersion.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202006000100005
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Cell suspension culture and plant regeneration of a Brazilian plantain, cultivar Terra. Repositório Alice
MORAIS-LINO, L.S.; SANTOS-SEREJO, J.A. dos; SILVA, S. de O. e; SANTANA, J.R.F. de; KOBAYASHI, A.K..
The objective of this study was to establish cell suspension culture and plant regeneration via somatic embryogenesis of a Brazilian plantain, cultivar Terra Maranhão, AAB. Immature male flowers were used as explant source for generating highly embryogenic cultures 45 days after inoculation, which were used for establishment of cell suspension culture and multiplication of secondary somatic embryos. Five semisolid culture media were tested for differentiation, maturation, somatic embryos germination and for plant regeneration. An average of 558 plants per one milliliter of 5% SCV (settled cell volume) were regenerated in the MS medium, with 11.4 µM indolacetic acid and 2.2 µM 6-benzylaminopurine. Regenerated plants showed a normal development, and no...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Musa; Culture media; Propagation; Somatic embryogenesis; Meio de cultura; Micropropagação; Embriogênese somática.
Ano: 2008 URL: http://www.alice.cnptia.embrapa.br/handle/doc/122219
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Cell suspension culture and plant regeneration of a Brazilian plantain, cultivar Terra PAB
Morais-Lino,Lucymeire Souza; Santos-Serejo,Janay Almeida dos; Silva,Sebastião de Oliveira e; Santana,José Raniere Ferreira de; Kobayashi,Adilson Kenji.
The objective of this study was to establish cell suspension culture and plant regeneration via somatic embryogenesis of a Brazilian plantain, cultivar Terra Maranhão, AAB. Immature male flowers were used as explant source for generating highly embryogenic cultures 45 days after inoculation, which were used for establishment of cell suspension culture and multiplication of secondary somatic embryos. Five semisolid culture media were tested for differentiation, maturation, somatic embryos germination and for plant regeneration. An average of 558 plants per one milliliter of 5% SCV (settled cell volume) were regenerated in the MS medium, with 11.4 µM indolacetic acid and 2.2 µM 6-benzylaminopurine. Regenerated plants showed a normal development, and no...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Musa; Culture media; Propagation; Somatic embryogenesis.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2008001000010
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Characterization of the third SERK gene in pineapple (Ananas comosus) and analysis of its expression and autophosphorylation activity in vitro Genet. Mol. Biol.
Ma,Jun; He,Yehua; Hu,Zhongyi; Xu,Wentian; Xia,Jingxian; Guo,Cuihong; Lin,Shunquan; Chen,Chengjie; Wu,Chenghou; Zhang,Junli.
Two somatic embryogenesis receptor-like kinase genes (identified as AcSERK1 and AcSERK2) have previously been characterized from pineapple (Ananas comosus). In this work, we describe the characterization of a third gene (AcSERK3) in this family. AcSERK3 had all the characteristic domains and shared extensive sequence homology with other plant SERKs. AcSERK3 expression was studied by in situ hybridization and quantitative real-time PCR to analyze its function. Intense in situ hybridization signals were observed only in single competent cells and competent cell clusters; no hybridization signal was detected in the subsequent stages of somatic embryogenesis. AcSERK3 was highly expressed in embryogenic callus compared to other organs, e.g., 20-80 fold more...
Tipo: Info:eu-repo/semantics/article Palavras-chave: AcSERK3; Autophosphorylation activity; Expression analysis; Prokaryotic expression; Somatic embryogenesis.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000400009
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Clonal Arabica coffee resistant to Meloidogyne paranaensis and damage threshold on plants development Scientia Agricola
Fatobene,Bárbhara Joana dos Reis; Gonçalves,Wallace; Oliveira,Claudio Marcelo Gonçalves; Guerreiro Filho,Oliveiro.
ABSTRACT: Plants of Coffea arabica Icatu IAC 925 germplasm show resistance to Meloidogyne paranaensis under field conditions, but with variable rates of segregation. One mother plant, IAC 3, of Icatu IAC 925 was evaluated under controlled conditions through the reaction of its clones, obtained by somatic embryogenesis. Reproduction of nematodes in clones were compared with an open-pollinated progeny from the same mother plant, C. arabica cultivar Mundo Novo IAC 515-20, susceptible to nematodes, and Clone IAC 1, also susceptible and obtained by somatic embryogenesis. Open-pollinated progeny showed segregation to resistance and was classified as susceptible according to the reproduction factor. The average number of nematodes (eggs and J2) per gram of roots...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Root-knot nematodes; Resistant variety; Somatic embryogenesis.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162019001300227
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Coffee biotechnology Braz. J. Plant Physiol.
De Los Santos-Briones,César; Hernández-Sotomayor,S. M. Teresa.
In the last three decades, interest has turned to in vitro cell culture in different areas of coffee research. In vitro techniques have been applied not only for coffee improvement through genetic transformation but also to study various aspects in coffee cells such as chemical (caffeine synthesis and the production of coffee aroma), physiological and more recently, biochemical aspects. The most important advances obtained to date on in vitro coffee techniques in fields like biochemistry, physiology, regeneration systems and genetic engineering, are presented and discussed.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Coffea; Coffee; Genetic transformation; In vitro tissue culture; Regeneration; Somatic embryogenesis.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202006000100015
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Comparison of Economic Efficiency between in Vitro and Field Methods for Vegetative Propagation of Coffea Canephora. Repositório Alice
SANTOS, M. R. A. dos; SOUZA, C. A. de; ROCHA, J. F. da; ARAUJO, L. V. de; ESPINDULA, M. C..
The objective of this study was to approach the viability of the two forms of propagation, comparing cost and time for the production of new plantlets and number of plantlets produced in each propagation system..
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Coffee; Somatic embryogenesis; Rooting of cuttings; Production cost; Café conilon; Embriogênese somática; Enraizamento de estacas; Custo de produção.
Ano: 2015 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1020428
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Date palm micropropagation: Advances and applications Ciência e Agrotecnologia
Al-Khayri,Jameel Mohammed; Naik,Poornananda Madhava.
ABSTRACT Date palm (Phoenix dactylifera L.) is a fruit tree resilient to adverse climatic conditions predominating in hot arid regions of the Middle East and North Africa. The date fruit contains numerous chemical components that possess high nutritional and medicinal values. Traditional propagation by offshoots is inefficient to satisfy current demands for date palm trees. Alternatively, micropropagation provides an efficient means for large-scale propagation of date palm cultivars. Both somatic embryogenesis and organogenesis, either directly or indirectly though the callus phase, have been demonstrated in date palm in vitro regeneration. Culture initiation commonly utilizes shoot-tip explants isolated from young offshoots. Recently, the immature...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cryopreservation; Somatic embryogenesis; Somaclonal variation; Organogenesis; Molecular marker..
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542017000400347
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Direct somatic embryogenesis and organogenesis pathway of plant regeneration can seldom occur simultaneously within the same explant of sorghum Open Agri
Girijashankar, V..
Palavras-chave: Somatic embryos; Transgenics; Somatic embryogenesis; Auxins; Regeneration; Protocols; Sorghum; Cytokinins; Embryonic development; Transgenic plants.
Ano: 2007 URL: http://agropedia.iitk.ac.in/openaccess/?q=node/3300
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Efeitos do metano sulfonato de etila sobre a sobrevivência e capacidade de regeneração de embriões somáticos de bananeira. Repositório Alice
MOREIRA, C. V.; SILVA, S. de O. e; SEREJO, J. A. dos S.; LINO, L. S. M.; NEPOMUCENO, C. F..
O presente estudo tem como objetivo avaliar a sensibilidade e o desenvolvimento de embriões somáticos de bananeira ?Tropical? submetidos ao tratamento com Metano Sulfonato de Etila (EMS) como agente mutagênico.
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Embriogênese somática; Musa spp; Banana; Mutação somática; Somatic mutation; Somatic embryogenesis.
Ano: 2014 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1006063
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Effect of carbon source on morphology and histodifferentiation of Araucaria angustifolia embryogenic cultures BABT
Steiner,Neusa; Vieira,Felipe do Nascimento; Maldonado,Sara; Guerra,Miguel Pedro.
The aim of the present work was to establish in vitro conditions for the induction, stabilization and proliferation of embryogenic cultures of A. angustifolia. Pre-cotyledonary staged zygotic embryos inoculated BM medium supplemented with 5 µM 2,4- D, 2 µM BAP and Kin, and 3% maltose or sucrose resulted in 66.7% induction rate. The rate of induction of embryogenic cultures was affected by the carbon source, as well the multiplication and morphology of the embryogenic cultures. Embryogenic cultures maintained in BM medium with maltose presented bipolar morphology. Globular somatic embryos were obtained BM medium with 9% (PEG) and (9%) maltose. These results could establish an in vitro regenerative protocol towards the conservation and improvement of this...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cell competence; Somatic embryogenesis; Carbohydrate; Conifers.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000800005
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Efficient In Vitro Somatic Embryogenesis and Plant Regeneration from Mature and Immature Embryos of Wheat ( Triticum aestivum L.) BABT
Ahmadpour,Roghayeh; Zare,Nasser; Asghari-Zakarta,Rasool; Sheikhzadeh,Parisa.
ABSTRACT An efficient regeneration system is a pre-requisite for the application of genetic transformation and functional genomics study of important plants. In this study, the effect of different factors (plant growth regulators, casein hydrolysate, aspartic acid and ascorbic acid) on in vitro embryogenesis and regeneration of Arta, Bahar and Zagros cultivars from mature and immature explants were investigated. Immature and mature embryos were dissected from disinfected seeds 20-25 days after pollination and imbedded mature seeds, respectively, and cultured on MS (Murashige and Skoog) medium supplemented with different compounds. The results showed that immature embryos expose high capacity of embryogenesis and regeneration in comparison with mature...
Tipo: Info:eu-repo/semantics/article Palavras-chave: In vitro culture; Plant growth regulators; Somatic embryogenesis; Wheat.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100206
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Embryogenic potential of the callus of gabirobeira, Campomanesia adamantium (Cambess) O. Berg Biological Sciences
Rossato, Marieli; Schumacher, Pedro Vitor; Costa Netto, Antônio Paulino da; Stein, Vanessa Cristina; Reis, Edésio Fialho dos; Vilela, Marlúcia Souza Pádua; Paiva, Luciano Vilela.
Campomanesia adamantium is a native plant species of Brazilian Cerrado with diverse economic potential and great medicinal importance. Its sexual propagation is impaired by the recalcitrance of its seeds, which prevents effective and profitable propagation. With the purpose of establishing commercial crops and minimizing the extractive use of vegetal resources, the aim of the present study was to induce embryogenic calli in nodal segments of gabirobeira, and to determine and characterize their embryogenic phase through the establishment of a growth curve based on cellular characteristics. Calli were induced using nodal segments inoculated in WPM culture medium without the addition of hormones (control) and with different concentrations of 2,4-D, IAA, IBA,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Somatic embryogenesis; Growth regulator; Callus.
Ano: 2019 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/46358
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Encapsulated Somatic Embryos and Zygotic Embryos for Obtaining Artificial Seeds of Rauli-Beech (Nothofagus alpina (Poepp. & Endl.) Oerst.) Chilean J. Agric. Res.
Cartes R,Priscila; Castellanos B,Hermes; Ríos L,Darcy; Sáez C,Katia; Spierccolli H,Scarlette; Sánchez O,Manuel.
Somatic and zygotic embryos from mature seeds of rauli-beech, Nothofagus alpina (Poepp. & Endl.) Oerst., were encapsulated in different artificial endosperms in order to generate a cover that fulfills the function of nourishment and protection of the embryos, facilitating their later germination. The content of sodium alginate varied by 4%, 3%, and 2%, as did the immersion time in calcium chloride (CaCl2), which acts as complexing agent. The artificial endosperm components of the Murashige and Skoog medium (MS) were added, supplemented with 0.5 mg L-1 indolacetic acid (IAA), 0.5 mg L-1 naphthaleneacetic acid (NAA), 2 mg L-1 6-benzylaminopurine (BAP) and 30 g L-1 sucrose. The germinative behaviors of encapsulated somatic and zygotic embryos were...
Tipo: Journal article Palavras-chave: Rauli-beech; Somatic embryogenesis; Synthetic seed; Sodium alginate.
Ano: 2009 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392009000100014
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