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Beneficial Effects of Angiotensin II AT1 Blocker on Cardiovascular Adverse Remodeling Due to Nitric Oxide Synthesis Blockade International Journal of Morphology
Fernandes-Santos,Caroline; Mendonça,Leonardo de Souza; Mandarim-de-Lacerda,Carlos Alberto.
We studied with morphological tools the effects of different doses of Losartan upon the cardiovascular remodeling in nitric oxide deficient rats. At 15 weeks of age, thirty Wistar rats were separated in six groups: control (C), L-NAME (LN), and four groups were LN was given plus Losartan at different doses (1, 5, 20 and 40 mg/kg/day). The L-NAME was given for 9 weeks, the Losartan administration starting on the 2nd week of experiment. We studied the heart, thoracic aorta and superior mesenteric artery with light microscopy and stereology. The blood pressure (BP) increased since the first week of L-NAME administration, the Losartan treatment at doses of 20 and 40 mg/kg/day was efficient to reduce BP after the 7th week of treatment. The cardiac adverse...
Tipo: Journal article Palavras-chave: Losartan; Angiotensin II; L-NAME; Hypertension; Renin-angiotensin system; Stereology; L-NAME.
Ano: 2006 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022006000400003
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Beneficial effects of exercise training (treadmill) on insulin resistance and nonalcoholic fatty liver disease in high-fat fed C57BL/6 mice BJMBR
Marques,C.M.M.; Motta,V.F.; Torres,T.S.; Aguila,M.B.; Mandarim-de-Lacerda,C.A..
C57BL/6 mice develop signs and symptoms comparable, in part, to the human metabolic syndrome. The objective of the present study was to evaluate the effects of exercise training on carbohydrate metabolism, lipid profile, visceral adiposity, pancreatic islet alterations, and nonalcoholic fatty liver disease in C57BL/6 mice. Animals were fed one of two diets during an 8-week period: standard (SC, N = 12) or very high-fat (HF, N = 24) chow. An exercise training protocol (treadmill) was then established and mice were divided into SC and HF sedentary (SC-Sed, HF-Sed), exercised groups (SC-Ex, HF-Ex), or switched from HF to SC (HF/SC-Sed and HF/SC-Ex). HF/HF-Sed mice had the greatest body mass (65% more than SC/SC-Sed; P < 0.0001), and exercise reduced it by...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Obesity; Lipid metabolism; Metabolic syndrome; Aerobic training; Fatty liver; Stereology.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000500008
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Comparison of dietary phospholipids and neutral lipids: effects on gut, liver and pancreas histology in Atlantic cod (Gadus morha L.) larvae ArchiMer
Wold, P. -a.; Hoehne-reitan, K.; Cahu, Chantal; Zambonino, Jose-luis; Rainuzzo, J.; Kjorsvik, E..
The aim of the present study was to compare effects of dietary n-3 highly unsaturated fatty acids (HUFA) being incorporated in the phospholipid (PL) or in the neutral lipid (NL) fraction of the larval feed, on larval growth and histology of digestive organs in Atlantic cod (Gadus morhua L.) larvae. Three isoproteic and isolipidic diets, labelled according to the percentage of n-3 docosahexaenoic acid and eicosapentaenoic acid contained in NL1 or in PL1 and PL3 of the diets, were fed to cod larvae from 17 days post hatching (dph) to 45 dph. In the liver, hepatocytes and their nuclei were smaller in NL1 larvae compared with the PL larvae; the mitochondrial membrane structures were less dense and the amount of lipids observed in the liver was significantly...
Tipo: Text Palavras-chave: Stereology; Phospholipids; Nutrition; Lipid class; Larvae; Histology; Diet; Cod.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/2009/publication-6777.pdf
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Determination of Lateral Ventricle and Brain Volume in Children with Stereological Method Using MRI International Journal of Morphology
Karacan,Keziban; Kosar,Mehmet Ilkay; Çimen,Mehmet; Solak,Orhan; Sahin,Bünyamin.
Although brain development is most active during the intrauterina period of life, the processes of myelination and arborization affect the structure of the brain throughout childhood and adolescence. Brain development is also very active in the early years of a child's life, and continues to be so for approximately 15 years after gestation. Volumetric changes in the brain are effected by sex. Understanding the variability of human brain volume during development is important for the interpretation of childhood neuroimaging studies. Hence the aim of this study is to determine the effects of sex difference on brain volume (BV), lateral ventricle volume (LV) and the ratio of brain volume and lateral ventricle volumes as a percentage (RLBV%) of 90 healthy...
Tipo: Journal article Palavras-chave: Brain; Lateral ventricles; Magnetic resonance imaging; Stereology.
Ano: 2013 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022013000100035
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Disector Z-axis mechanical method for stereology Anais da ABC (AABC)
Xavier-Vidal,Ricardo.
The purpose of this work is to calibrate a not expensive microscope to be applied in Optical Disector estimation. The evaluation of "Z-axis" bias and the "Z-axis" calibration were made utilizing a cover slip and a "manual digimatic outside micrometer scale" (Mitutoyo, Japan). Calibrating the cover slip we performed the calibration of the "Z-axis" of a microscope. In the cover slip two lines were painted with different colors using a pen glass. A blue line was painted on the up surface and another line (red) on the bottom surface of the cover slip forming a cross. Two metal rings with one palette welded in each were adapted in the microscope. Other palette was welded in the gross focuses in order to restrict the route of the fine focuses (Z-axis study)...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Stereology; Disector; Optical disector; Morphometry; Z-axis.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652010000200028
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Early effects of estrogen on the rat ventral prostate BJMBR
García-Flórez,M.; Oliveira,C.A.; Carvalho,H.F..
Complex interactions between androgen and estrogen (E2) regulate prostatic development and physiology. We analyzed the early effects of a high single dose of E2 (25 mg/kg body weight) and castration (separately or combined) on the adult 90-day-old male Wistar rat ventral prostate. Androgen levels, prostate weight, and the variation in the relative and absolute volume of tissue compartments and apoptotic indices were determined for 7 days. Castration and exogenous E2 markedly reduced ventral prostate weight (about 50% of the control), with a significant reduction in the epithelial compartment and increased stroma. The final volume of the epithelium was identical at day 7 for all treatments (58.5% of the control). However, E2 had an immediate effect, causing...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Apoptosis; Estrogen; Prostate; Stereology; Tissue kinetics.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000400002
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EFFECT OF CADMIUM ON RAT MAXILLARY MOLAR JUNCTIONAL EPITHELIUM DURING LACTATION: EFECTO DEL CADMIO SOBRE EL EPITELIO DE LA ZONA DE UNIÓN MAXILO-MOLAR DE RATAS DURANTE LA LACTANCIA International Journal of Morphology
Ribas,Paulo; Lopes,Ruberval A.; Sala,Miguel A.; Ribas,Lucia M.R.; de Mattos,Maria da Glória C.; Semprini,Marisa; Watanabe,Ii-Sei; Regalo,Simone C.H..
Cadmium in the air, drinking water and food has the potential to affect the health of people, mainly those who live in highly industrialized regions. Cadmium affects placental functions, can cross the placental barrier and directly disturbs fetal development. It is also excreted into milk. The organism is particularly susceptible to cadmium exposure at perinatal period. The effect on rat maxillary molar junctional epithelium a continuous exposure to drinking water containing low level of cadmium during lactation was studied. Female rats received drinking water ad libitum containing 300 mg/l CdCl2 throughout the whole lactation. Control animals received a similar volume of water without cadmium. Lactent rats (21 day-old) were killed by lethal dose of...
Tipo: Journal article Palavras-chave: Oral mucosae; Junctional epithelium; Cadmium; Lactation; Morphometry; Stereology.
Ano: 2004 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022004000400003
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Effect of Photoperiod and Melatonin on Volume and Cellular Parameters of Testis in Pinealectomized Rats International Journal of Morphology
Akosman,Murat Sirri; Özdemir,Vural; Taskiran,Nihal; Akalan,Mehmet Aydin; Bülbül,Aziz.
Mammalian reproductive axis is regulated by the combination of three fundamental tissues of neuroendocrine system including hypothalamus, hypophysis and gonads. In recent years, pineal gland has been included in this axis. The aim of the present study was to investigate the effect of 12L (Light):12D (Dark) photoperiod and melatonin administration (0.5 mg/kg/day; subcutaneously) on testicular volume and cellular parameters of testis at the pinealectomized (PE) rats. For this aim, twelve adult rats were firstly pinealectomized and then divided into two groups as GI and GII randomly. The GI rats served as control group and received only normal saline, whereas GII rats were the melatonin administered group. It was found that the total testicular volume,...
Tipo: Journal article Palavras-chave: Cavalieri Method; Melatonin; Optical Fractionator; Pinealectomy; Stereology; Testis.
Ano: 2013 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022013000300046
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Effect of praziquantel administration on hepatic stereology of mice infected with Schistosoma mansoni and fed a low-protein diet BJMBR
Barros,L.A.; Costa-Silva,M.; Biolchini,C.L.; Neves,R.H.; Machado-Silva,J.R..
A study was undertaken to investigate the effect of administering praziquantel (PZQ), focusing on the liver stereological findings of malnourished mice infected with Schistosoma mansoni. Thirty female Swiss Webster mice (age: 21 days; weight: 8-14 g) were fed either a low-protein diet (8%) or standard chow (22% protein) for 15 days. Five mice in each group were infected with 50 cercariae each of the BH strain (Brazil). PZQ therapy (80 mg/kg body weight, per day) was started on the 50th day of infection and consisted of daily administration for 5 days. Volume density (hepatocytes, sinusoids and hepatic fibrosis) was determined by stereology using a light microscope. Body weight gain and total serum albumin levels were always lower in undernourished mice....
Tipo: Info:eu-repo/semantics/other Palavras-chave: Schistosoma mansoni; Low-protein diet; Stereology; Praziquantel.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000900007
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Effect of Sodium Cyclamate on the Rat Fetal Exocrine Pancreas: a Karyometric and Stereological Study International Journal of Morphology
Martins,Alex Tadeu; Santos,Fabiano de Sant´Ana dos; Scannavino,Fábio Luiz Ferreira; Pires,Juliana Rico; Zuza,Elizângela Partata; Junior,João Armando Padovani; Azoubel,Reinaldo; Di Mateo,Miguel Angel Sala; Lopes,Ruberval Armando.
The cyclamate, a sweetner substance derived from N-cyclo-hexyl-sulfamic acid, is largely utilized as a non-caloric artificial edulcorant in foods and beverages as well as in the pharmaceutical industry. The objective of this study was to evaluate karyometric and stereological alterations in the rat fetal pancreas resulting from the intraperitoneal administration of sodium cyclamate. The exocrine pancreas of ten fetuses of rats were evaluated, five treated and five controls chosen at random, in which five rats that received from the 10th to 14th days of pregnancy an intraperitoneal daily injection of sodium cyclamate at 60 mg/Kg of body weight during 5 days. At the 20th day of gestation, the animals were removed and weighed, as were their placentas; the...
Tipo: Journal article Palavras-chave: Sodium cyclamate; Exocrine pancreas; Karyometry; Stereology.
Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022010000300038
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Effect of Sodium Cyclamate on the Rat Fetal Liver: A Karyometric and Stereological Study International Journal of Morphology
Martins,Alex Tadeu; Azoubel,Reinaldo; Lopes,Ruberval A.; di Matteo,Miguel A. Sala; de Arruda,José Germano Ferraz.
The cyclamate, a sweetner substance derived from N-cyclo-hexyl-sulfamic acid, is largely utilized as a non-caloric artificial edulcorant in foods and beverages as well as in the pharmaceutical industry. The objective of this study was to evaluate fetal hepatic karyometric and stereological alterations in the rat fetal liver resulting from the intraperitoneal administration of sodium cyclamate. The livers of ten rats were evaluated, five treated and five controls chosen at random, in which five rats that received from the 10th to 14th days of pregnancy an intraperitoneal daily injection of sodium cyclamate at 60 mg/Kg of body weight during 5 days. At the 20th day of gestation, the animals were removed and weighed, as were their placentas, on a precision...
Tipo: Journal article Palavras-chave: Sodium cyclamate; Fetal liver; Karyometry; Stereology.
Ano: 2005 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022005000300005
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Effects of Oral Nicotine on Rat Liver Stereology International Journal of Morphology
Valenca,Samuel Santos; Gouveia,Lucas; Alves Pimenta,Wagner; Porto,Luís Cristóvão.
Nicotine is the more abundant component in cigarette smoke. Because nicotine is first metabolized in the liver, our aim was to investígate the effects of nicotine on this organ by biochemical and stereological methods. Male Wistar rats were treated with oral nicotine (ON) diluted in drinking water during 32 days. The control group was treated with drinking water in the same period. Rats were sacrificed 24 hours after last day, the blood was collected and the liver was removed. Lipidogram was performed by enzymatic method and collagen fibers, fat globules and hepatocytes were count in the liver by stereological methods. We observed in control group preserved hepatocytes, with no presence of inflammatory cells. However in the ON group was possible to observe...
Tipo: Journal article Palavras-chave: Nicotine; Rat; Liver; Stereology.
Ano: 2008 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022008000400037
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Effects of Preweaning Polysensorial Enrichment upon Development of the Parietal Cortical Plate of Undernourished Rats: A Stereological Study International Journal of Morphology
González,Héctor; Adaro,Luis; Hernández,Alejandro; Fernández,Víctor.
This investigation was undertaken in order to quantify the effects of early polysensorial enrichment on the development of cortical pyramids, located in the parietal cortex of rats simultaneously submitted to protein-energy undernutrition. A short period of stimulation during suckling significantly decreases the cellular density in the cortical plate (phylogenetic-ontogenetic evolutionary index). Results suggest that the cerebral cortex develops according to a sophisticated neuronal network, which exhibits a notable degree of structural specificity, regulated by genetic and environmental clues. The most obvious prediction is that environmental influences are probably transduced as a structural expression in the developing parietal cortical plate.
Tipo: Journal article Palavras-chave: Environmental enrichment; Cortical gradients; Undernutrition; Parietal cortex; Stereology.
Ano: 2014 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022014000400016
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ENALAPRIL AND VERAPAMIL ATTENUATE THE AORTIC WALL REMODELING IN NITRIC OXIDE DEFICIENCY International Journal of Morphology
Meirelles Pereira,Leila Maria; Gonçalves Bezerra,Daniele; Mandarim-de-Lacerda,Carlos Alberto.
Forty mature male rats (Wistar strain) were maintained alive for 40 days, separated in four groups of five rats each (Control, L-NAME, L-NAME + Enalapril, L-NAME + Verapamil). Three aortic wall parameters were analyzed, as follows: the Q A(SMN) (the number of the tunica media smooth muscle nuclear profiles per area), the S V(lamellae) (surface density of the aortic lamellae), and the aortic wall thickness (AWT). Rats of the L-NAME group showed high blood pressure and cardiac hypertrophy. The AWT, the Q A(SMN) and the S V(lamellae) showed the same tendency among the groups. The increased AWT, or the reduced Q A(SMN) and the S V(lamellae) in l-NAME rats were not completely prevented by the administration of enalapril or verapamil. Hypertension causes the AWT...
Tipo: Journal article Palavras-chave: Nitric oxide; Aorta; Enalapril; Verapamil; Stereology.
Ano: 2003 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022003000100002
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End-To-Side Loop Neurorrhaphy: Axonal Comparative Stereological Study International Journal of Morphology
Boer,Nagib Pezati; Viterbo,Fausto; Faleiros,Humberto Regis; Batigalia,Fernando; Boer,Ana Luísa Ricci; Carvalho Jr,Altair Antonio.
Accidents or diseases can affect the peripheral part of the nervous system, which raises clinical and surgical therapies, among others. In this context, the technique of end-to-side neurorrhaphy is a treatment option, yet its modification loop needs some additional efficacy studies. The purpose of this study was to compare, among rats, stereological results (axons volume density) after end-to-side neurorrhaphy and after end-to-side loop neurorrhaphy. Thirty Wistar rats were used, divided into six groups (five animals per group), consisting of two control groups (for the fibular and tibial nerves), two study groups for the fibular nerve (one with an end-to-side neurorrhaphy, and the other with an end-to-side loop neurorrhaphy) and two study groups for the...
Tipo: Journal article Palavras-chave: Nerve; Axon; Stereology; Neurorrhaphy.
Ano: 2014 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022014000300032
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Evaluation of Lumbar Vertebral Body and Disc: A Stereological Morphometric Study International Journal of Morphology
Gocmen-Mas,Nuket; Karabekir,Hamit; Ertekin,Tolga; Edizer,Mete; Canan,Yazici; Duyar,Izzet.
Anterior access to the L1-L5 vertebrae and disc spaces can be technically challenging, frequently requiring the use of an approach to a surgeon for an adequate exposure. The technique is used for lesion excision, corpectomy, vertebral body reconstruction with cages, realignment, and/or plating or screwing. For a successful anterior approach and a suitable instrumental design via screw, adequate morphometric knowledge about body of lumbar vertebrae and disc spaces and standardized volumetric data are also required for neurosurgeons. We aimed morphometric and volumetric evaluation of lumbar bodies and discs to contribute to a safe anterior approach during surgery. We evaluated vertebral body and disc morphometry using stereology in right-handed 25 adult...
Tipo: Journal article Palavras-chave: Lumbar vertebra; Body; Morphometry; Volumetry; Stereology; Anterior access.
Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022010000300028
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Exendin-4 effects on islet volume and number in mouse pancreas BJPS
Khorsandi,Layasadat; Nejad-Dehbashi,Fereshteh.
The aim of this study was to evaluate Exendin-4 (EX-4) effects on islet volume and number in the mouse pancreas. Thirty-two healthy adult male NMRI mice were randomly divided into control and experimental groups. EX-4 was injected intraperitoneally (i. p.) at doses of 0.25 (E1 group), 0.5 (E2 group), and 1 µg/kg (E3 group), twice a day for 7 consecutive days. One day after the final injection, the mice were sacrificed, and the pancreas from each animal dissected out, weighed, and fixed in 10% formalin for measurement of pancreas and islet volume, and determination of islet number by stereological assessments. There was a significant increase in the weight of pancreases in the E3 group. Islet and pancreas volumes in E1 and E2 groups were unchanged compared...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Exendin-4/effects; Exendin-4/experimental study; Pancreas/effects fo Exendin-4; Islet/hypertrophy; Stereology.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000400014
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Gametogenesis, reproductive investment, and spawning behavior of the Pacific giant oyster Crassostrea gigas: evidence of an environment-dependent strategy ArchiMer
Enriquez-diaz, M.; Pouvreau, Stephane; Chavez-villalba, J.; Le Pennec, M..
The progress of gametogenesis was studied in oysters Crassostrea gigas having the same origin (Tremblade), but cultured during 1 year in two distinctive French marine areas, the Baie des Veys and Marennes-Ol,ron. We assessed seasonal changes in the reproduction cycle on the basis of stereological techniques to estimate reproductive investment and measurement of gonad evolution area by quantitative histology. From a qualitative point of view, both oyster groups presented typical reproductive stages, but showed differential timing, in particular during the sequence of spawning and duration of the re-absorption stage. Oysters in Baie des Veys had a single partial spawning in August and a re-absorption stage that extended until winter. Oysters in...
Tipo: Text Palavras-chave: Stereology; Reproductive investment; Pacific oysters; Image analysis; Gametogenesis; Crassostrea gigas.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/2009/publication-7300.pdf
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High Concentration of Reticular Fibers in Periurethral Region of the Prostatic Urethra from Patients with Benign Prostatic Hyperplasia as Revealed by Stereology International Journal of Morphology
Manaia,Jorge H. M; Costa,Waldemar S; Sampaio,Francisco J. B; Figueiredo,Marcelo A; Cardoso,Gilberto P; Babinski,Marcio A.
The goal of this investigation was to analyze and quantify changes of the reticular fibers in the prostatic urethra of patients with benign prostatic hyperplasia (BPH) and compare with a control group. Prostatic urethra tissue samples were obtained from ten patients (age range 65 to 79 years, mean 66) with clinical symptoms of bladder outlet obstruction who had undergone open prostatectomy. The ten control group samples (urethral tissue samples from the transitional zone) were collected from prostates obtained during autopsy of accidental death adults of less than 25 years. The Vv of the reticular fibers was determined with stereologic methods from 25 random fields per sample using the point-count method with a M-42 grid test system. The quantitative data...
Tipo: Journal article Palavras-chave: Prostatic urethra; Reticular fibers; Stereology.
Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022012000300065
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High-Intensity Interval Training Beneficial Effects in Diet-Induced Obesity in Mice: Adipose Tissue, Liver Structure, and Pancreatic Islets International Journal of Morphology
Motta,Victor F; Aguila,Marcia B; Mandarim-de-Lacerda,Carlos A.
The study aimed to evaluate the impact of high-intensity interval training (HIIT) on adipose tissue, pancreatic islets and liver in mice fed high-fat diet. C57BL/6 male mice were fed one of two diets: standard chow (Lean group - LE) or a high-fat diet (Obese group ­ OB). After the first 12-weeks, the animals were divided into non-trained (LE-NT and OB-NT), trained groups (LE-T and OB-T), and started the exercise protocol. The HIIT protocol in the trained animals (LE-T and OB-T) compared to their counterparts (LE-NT and OB-NT) led to a reduction in size of the pancreatic islets (LE-T vs. LE-NT -40 %, OB-T vs. OB-NT -22 %) and to an increase in insulin immunodensity in pancreatic islet (LE-T vs. LE-NT +35 %, OB-T vs. OB-NT +31 %). Apart from the above...
Tipo: Journal article Palavras-chave: HIIT; Obesity; Insulin Resistance; Hepatic Steatosis; Stereology.
Ano: 2016 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022016000200042
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