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Formulation of economical microbial feed using degraded chicken feathers by a novel Streptomyces sp: mitigation of environmental pollution BJM
Ramakrishnan,Jayapradha; Balakrishnan,Hariram; Raja,Selvaraj Thirupathi Kumara; Sundararamakrishnan,Natarajan; Renganathan,Sadagoban; Radha,Venkatesh Nagarajan.
A new Streptomyces sp. IF 5 was isolated from the feather dumped soil and found to have a tremendous keratinase activity. The strain enabled the degradation of the chicken feathers very effectively in 60 h. The 16S rRNA sequence of 1474 bp long was submitted to the National centre for Biotechnological information. The keratinolytic activity in the culture medium was 1181 U/ml. The release and analyses of sulphydryl groups in the culture medium evident the degradation activity by the Streptomyces sp. IF 5. The idea of the present study was to use the degraded chicken feathers as the substrate for the growth and cultivation of microorganisms. We have designed a very economical culture medium that includes the usage of some basal salts alone and degraded...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Keratinase activity; Streptomyces sp; Microbial feed; Chicken feather.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000300001
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Optimization of clavulanic acid production by Streptomyces daufpe 3060 by response surface methodology BJM
Marques,Daniela A. Viana; Cunha,Márcia N. Carneiro; Araújo,Janete M.; Lima-Filho,José L.; Converti,Attilio; Pessoa-Jr,Adalberto; Porto,Ana L. Figueiredo.
Clavulanic acid is a β-lactam antibiotic which has a potent β-lactamase inhibiting activity. In order to optimize its production by the new isolate Streptomyces DAUFPE 3060, the influence of two independent variables, temperature and soybean flour concentration, on clavulanic acid and biomass concentrations was investigated in 250 mL-Erlenmeyers according to a 2² central composite design. To this purpose, temperature and soybean flour (SF) concentration were varied in the ranges 26-34°C and 10-50 g/L, respectively, and the results evaluated utilizing the Response Surface Methodology. The experimental maximum production of clavulanic acid (629 mg/L) was obtained at 32°C and 40 g/L SF after 48 h, while the maximum biomass concentration (3.9 g/L) at 30°C and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Clavulanic acid; Response surface methodology; Production; Optimization; Streptomyces sp.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000200030
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Partial characterization of cold active amylases and proteases of Streptomyces sp. from Antarctica BJM
Cotârleţ,Mihaela; Negoiţă,Teodor Gh; Bahrim,Gabriela E; Stougaard,Peter.
The aim of this study was to isolate novel enzyme-producing bacteria from vegetation samples from East Antarctica and also to characterize them genetically and biochemically in order to establish their phylogeny. The ability to grow at low temperature and to produce amylases and proteases cold-active was also tested. The results of the 16S rRNA gene sequence analysis showed that the 4 Alga rRNA was 100% identical to the sequences of Streptomyces sp. rRNA from Norway and from the Solomon Islands. The Streptomyces grew well in submerged system at 20ºC, cells multiplication up to stationary phase being drastically increased after 120 h of submerged cultivation. The beta-amylase production reached a maximum peak after seven days, while alpha-amylase and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Streptomyces sp; East Antarctica; Cold-adapted strain; Cold active proteases and amylase.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000300005
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Production of α-amylase from Streptomyces sp. SLBA-08 strain using agro-industrial by-products BABT
Santos,Édilla Ribeiro dos; Teles,Zozilene Nascimento Santos; Campos,Núria Mariana; Souza,Diogo Angeli Jacinto de; Bispo,Aline Simões da Rocha; Nascimento,Rodrigo Pires do.
Approximately 1.5 trillion tons are the estimated yearly biomass production, making it an essentially unlimited source of raw material for environmentally friendly and biocompatible products transformed by microorganism, specially fungi and actinomycetes. Several lignocellulosic residues, such as sisal waste and sugarcane bagasse contain starch in their structures which could become important sources for the production of amylases. This study evaluated the production of amylolytic enzymes using Streptomyces sp. SLBA-08 strain, isolated from a semi-arid soil, according to their ability to grow on soluble starch as the sole carbon source. The effect of the carbon source (sisal waste and sugarcane bagasse) on α-amylase production was studied using submerged...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Α-amylases; Sugarcane bagasse; Sisal waste; Streptomyces sp.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000500020
Registros recuperados: 4
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