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Registros recuperados: 21
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A practical approach to the understanding and teaching of RNA silencing in plants Electron. J. Biotechnol.
Bazzini,Ariel A; Mongelli,Vanesa C; Hopp,H. Esteban; del Vas,Mariana; Asurmendi,Sebastián.
Gene silencing, also called RNA interference (RNAi) is a specific mechanism of RNA degradation involved in gene regulation, development and defense in eukaryotic organisms. It became an important subject in the teaching programs of molecular biology, genetics and biotechnology courses in the last years. The aim of this work is to provide simple and inexpensive assays to understand and teach gene silencing using plants as model systems. The use of transient and permanent transgenic plants for expressing reporter genes, like those derived from jellyfish green fluorescent protein (gfp) encoding gene, provides a nice, colorful and conclusive image of gene silencing. Three experimental approaches to evidence RNA silencing are depicted. In the first approach...
Tipo: Journal article Palavras-chave: Gene silencing; GFP; HC-Pro; PDS; Protocols; PTGS; Teaching; Transgenic; Transient expression; TRV; VIGS.
Ano: 2007 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000200002
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Advances in Apple Transformation Technology to Confer Resistance to Fungal Diseases in Apple Crops: A Chilean Perspective Chilean J. Agric. Res.
Polanco,Víctor; Paredes,Mario; Becerra,Viviana; Pérez,Eduardo.
Apple (Malus domestica Borkh.) is one of the most consumed fruit in the world. Genetic transformation is a key process to sustain this demand by permitting the potential enhancement of existing cultivars as well as the development of new cultivars resistant to pests, diseases, and storage problems that occur in the major production areas. This review summarizes the advances of genetic engineering applied to the development of resistant apple cultivars to fungus disease, with particular attention in the generation of apples resistant to Venturia inaequalis (Cooke) G.Winter, the main phytosanitary problem that affects apple crops in Chile.
Tipo: Journal article Palavras-chave: Apple; Fungal disease; Transgenic; Cisgenesis.
Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392010000200013
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Attitudes towards genomic: Research in four Latin American countries Electron. J. Biotechnol.
Rodríguez Yunta,Eduardo; Valdebenito Herrera,Carolina; Misseroni,Adelio; Fernández Milla,Lautaro; Outomuro,Delia; Schiattino Lemus,Irene; Ferrer Lues,Marcela; Lolas Stepke,Fernando.
The present reflection refers to data obtained about the social representations of genomic research and its applications through interviews with legislators and lawyers, biomedical researchers and civilians and the review of scientific and legal literature in four Latin American countries: Argentine, Chile, Mexico and Peru. Several issues are addressed: little access to prevention and therapeutic methods, lack of equity in health benefits, commercialization of gene sequences through patents which leads to commercial exploitation of underdeveloped countries, the possibility of physical or psychological damage or genetic discrimination, the possibility of genetic modifications or abortion for eugenic reasons, the necessity of safeguarding confidentiality,...
Tipo: Journal article Palavras-chave: Cloning; Genome; Social representations; Transgenic.
Ano: 2005 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582005000300003
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Biotechnology and Planted Forests: Assessment of Potential and Possibilities AgEcon
Sedjo, Roger A..
This paper addresses the potential impact of the introduction and development of biotechnology on planted forests. It includes a description of some recent innovations in forestry including the use of traditional breeding, and also more recent innovations involving biotechnology, including the development of clonal propagation and the use of modern molecular biology techniques. In addition to describing these innovations, the paper undertakes an assessment of their probable impact on future production of the forest industry, on the global timber supply, and on future markets for timber and wood products. The paper offers a description of recent innovations in tree breeding and biotechnology, including a discussion of innovations in agriculture that have...
Tipo: Working or Discussion Paper Palavras-chave: Biotechnology; Plantation forests; Genetic modification; Genetic research; Economic benefits; Transgenic; GMO; Resource /Energy Economics and Policy; Q21; Q23; Q16; O32; L73.
Ano: 1999 URL: http://purl.umn.edu/10862
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Caracterização genético-bioquímica de milho tropical transformado com o gene que codifica para a zeína. Repositório Alice
CARNEIRO, N. P.; CARNEIRO, A. A.; CARVALHO, C.H.S.; LOPES, M.A.; PAIVA, E..
2000
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Transgenico; Milho tropical; Zeinas; Metionina; Endosperma; Zea mays; Tropical maize; Transgenic; Zein; Endosperm.
Ano: 2000 URL: http://www.alice.cnptia.embrapa.br/handle/doc/484445
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Consumers Willingness to Pay to Avoid Transgenic Products AgEcon
Ribeiro, Francis Lee; de Noronha, Jose Ferreira; Noronha, Cassia Maria Silva.
The debate on transgenic, which is embebed in asymmetrical information, has as a focal point, the risks and uncertainties to them associated. Many are the concerns and questions about the risks for the human health and the environment. Considering the interests of the productive sector in the transgenic technology, the matter sets itself in terms of how fast it wants to be settled taking into consideration the incapability of establishing certainties about the damages or benefits within the process. With the recent liberation by the Brazilian government for its production the sector starts showing segmentation. Therefore, this issue reflects in the market itself. The question is: are the consumers willing to buy transgenic? As an attempt to answer this...
Tipo: Conference Paper or Presentation Palavras-chave: Transgenic; Contingent Valuation; Measure of Well-being; Demand and Price Analysis; Food Consumption/Nutrition/Food Safety.
Ano: 2005 URL: http://purl.umn.edu/24221
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Effects of high proline accumulation on chloroplast and mitochondrial ultrastructure and on osmotic adjustment in tobacco plants Agronomy
Borgo, Lucélia; Marur, Celso Jamil; Vieira, Luiz Gonzaga Esteves.
Proline accumulates in many organisms in response to abiotic stresses, such as drought and salinity. Reports have discussed the role of proline in relation to osmotic adjustment, even though no clear-cut evidence has yet been provided demonstrating this association as a defense mechanism against drought stress in plants. However, it has been indicated that the application of exogenous proline can damage the ultrastructure of chloroplasts and mitochondria. In this study, using transgenic tobacco plants carrying a mutant p5cs gene from Vigna aconitifolia under control of the CaMV 35S promoter, we show that the high levels of endogenous proline accumulated in leaves does not contribute to the osmotic adjustment in plants under water deficit conditions....
Tipo: Info:eu-repo/semantics/article Palavras-chave: 5.01.00.00-9 p5cs; Transgenic; Water deficit Melhoramento Vegetal.
Ano: 2015 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/19097
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EL PAPEL DEL ADMINISTRADOR DE AGRONEGOCIOS EN LA SEGURIDAD ALIMENTARIA AgEcon
Guerra E., Guillermo A.; Aguilar Valdes, Alfredo; Cabral Martell, Agustin; Alvarado Martinez, Luis Felipe; Alvarado Martinez, Tomas Everardo; Arras Vota, Ana Maria; Moreno Medina, Salomon.
For decades, food security has been a worldwide problem affecting the poorest in order to find a possible solution to this dilemma that not only continues, but constantly grows with the increase in population. Agriculture, as the main food supplier, has evolved and increased its productivity, and has expanded its agricultural frontiers, since previous decades, in its attempts to solve this problem. If predominant conventional agriculture (mainly dedicated to productivity) is complemented with substantial increase of transgenic, organic, agroecological, greenhouse and hydroponic crops, it will be more feasible to avoid the loss of forest, the damage and loss of soils and at the same time diminish food insecurity. Within this context, the agribusiness...
Tipo: Journal Article Palavras-chave: Food security; Transgenic; Organics; Agro ecological; Hydroponics; Greenhouse; Agribusiness manager.; Agribusiness.
Ano: 2011 URL: http://purl.umn.edu/99477
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Engenharia genética: conceitos básicos, ferramentas e aplicações. Infoteca-e
CORDEIRO, M. C. R..
ABSTRACT: Basic concepts and methodologies concerning genetic engineering are presented in this review and is directed to people or professionales not familiarized to it. Moreover, it can be a document to clarity what is genetic engineering, the importance of its contributions to modern agriculture, medicine or livestock research and to support discussions on genetic research ethics. Covers information on structure of nucleic acids, types, functions and main processes such us DNA duplication, transcription and traduction. Important methodologies are covered about DNA/RNA extraction, electrophoresis, PCR, nucleic acid libraries production, cloning, sequencing, gene characterization in connection to main historical events of genetic engineering. It...
Tipo: Documentos (INFOTECA-E) Palavras-chave: Engenharia genética; Biotecnologia; Marcador genético; DNA; RNA; Ácido nucléico; Gene; Biologia molecular; Clonagem; Transgênico; Genetic engineering; Biotechnology; Genetic markers; Nucleic acids; Genes; Molecular biology; Cloning; Transgenic.
Ano: 2003 URL: http://www.infoteca.cnptia.embrapa.br/handle/doc/568132
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Ensaio de competição de cultivares tolerantes ao glifosato da rede soja sul de pesquisa, safra 2009/10. Infoteca-e
BERTAGNOLLI, P. F.; COSTAMILAN, L. M.; NICOLAU, M.; VERNETTI JUNIOR, F. de J.; STECKLING, C.; ROVERSI, T.; RUBIN, S. de A. L.; GONÇALVES, J. A.; CASTRO, R. L. de; GABE, N. L.; OLIVEIRA, M. A. R. de; VICENTE, D.; MATZENBACHER, R.; GIASSON, N. F.; BAGATINI, N. P.; SILVA, A. E. L. da..
2010
Tipo: Capítulo em livro técnico (INFOTECA-E) Palavras-chave: Soybean; Transgenic.
Ano: 2010 URL: http://www.infoteca.cnptia.embrapa.br/handle/doc/882340
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Ex-Ante Evaluation of Drought Tolerant Varieties in Eastern and Central Africa AgEcon
Kostandini, Genti; Mills, Bradford F.; Mykerezi, Elton.
Tipo: Conference Paper or Presentation Palavras-chave: Transgenic; Drought Tolerance; Household Benefits; Consumer/Household Economics; Crop Production/Industries.
Ano: 2008 URL: http://purl.umn.edu/49372
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Genomics and genetic transformation in arachis. Repositório Alice
BRASILEIRO, A. C. M.; ARAUJO, A. C. G.; LEAL BERTIOLI, S. C.; GUIMARAES, P. M..
2014
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Arachis; Functional genomics; Transgenic; Genome; Peanut.
Ano: 2014 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1006434
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Histocytological examination on organogenesis and somatic embryogenesis of HBsAg-transgenic cherry tomato mutant Phyton
Guan,Z-J; Guo,B; Huo,Y-L; Dai,J-K; Wei,Y-H.
The initiation and development of organogenic buds and somatic embryos in HBsAg-transgenic cherry tomato (Lycopersicum esculentum var. cerasiforme) mutant were studied histologically. The leaf explants of the mutant were cultured on Murashige & Skoog (MS) basal medium supplemented with 6-BA 1.0 mg/L and IAA 0.05 mg/L for callus induction. Histological studies on the leaf explants of the mutant at various developmental stages revealed that organogenic buds first appeared in the axillary position of explants on the 14th cultured day, and then somatic embryos formed in the same mutant explants after 35 days of culture. Transmission electron microscopy and scanning electron microscopy indicated that there were significant changes in morphology and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Histocytological; Organogenesis; Somatic embryogenesis; HBsAg; Transgenic; Cherry tomato; Mutant.
Ano: 2012 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-56572012000100008
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ISSUES IN DEVELOPMENT AND ADOPTION OF GENETICALLY MODIFIED (GM) WHEATS AgEcon
Wilson, William W.; Janzen, Edward L.; Dahl, Bruce L.; Wachenheim, Cheryl J..
Development of genetically modified (GM) wheat varieties is proceeding; however, several critical issues remain the focus of contention. This project summarizes the current state of knowledge on some of these critical issues for commercialization of GM wheats. Background on the evolution of GM Wheats is presented. Then, agronomic adoption and competitiveness of GM crops; research on GM traits in wheat; consumer acceptance of GM crops (a separate section is included on issues related to consumer acceptance of GM crops); regulatory issues and status; international trade; testing, segregation, and identity preservation; and production and marketing risks are examined. Finally, there is a description of the likely marketing system to evolve and a...
Tipo: Working or Discussion Paper Palavras-chave: Wheat; Genetic modification; Transgenic; Marketing; Crop Production/Industries; Research and Development/Tech Change/Emerging Technologies.
Ano: 2003 URL: http://purl.umn.edu/23497
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Produção de plantas transgênicas de milho tropical via biobalística. Repositório Alice
CARNEIRO, A. A.; CARVALHO, C.H.S.; CARNEIRO, N. P.; VASCONCELOS, M. J. V.; LOPES, M.A.; PAIVA, E..
2000
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Biobalistica; Milho; Transgenico; Qualidade nutricional; Zea mays; Maize; Transgenic; Nutritional quality; Biobalistics.
Ano: 2000 URL: http://www.alice.cnptia.embrapa.br/handle/doc/483859
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Produção de plantas transgênicas de milho tropical via biobalística. Repositório Alice
CARNEIRO, A. A.; CARVALHO, C.H.S.; CARNEIRO, N. P.; VASCONCELOS, M. J. V.; LOPES, M.A.; PAIVA, E..
2000
Tipo: Artigo em anais de congresso (ALICE) Palavras-chave: Milho; Transgenico; Biobalistica; Qualidade nutricional; Zea mays; Maize; Transgenic; Biobalistics; Nutritional quality.
Ano: 2000 URL: http://www.alice.cnptia.embrapa.br/handle/doc/484011
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Transgenic animal models for the functional analysis of vasoactive peptides BJMBR
Bader,M..
The interplay of vasoactive peptide systems is an essential determinant of blood pressure regulation in mammals. While the endothelin and the renin-angiotensin systems raise blood pressure by inducing vasoconstriction and sodium retention, the kallikrein-kinin and the natriuretic-peptide systems reduce arterial pressure by eliciting vasodilatation and natriuresis. Transgenic technology has proven to be very useful for the functional analysis of vasoactive peptide systems. As an outstanding example, transgenic rats overexpressing the mouse Ren-2 renin gene in several tissues become extremely hypertensive. Several other transgenic rat and mouse strains with genetic modifications of components of the renin-angiotensin system have been developed in the past...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Renin-angiotensin system; Transgenic; Animal models; Knockout.
Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000900011
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Transgenic Trees: Implementation and Outcomes of the Plant Protection Act AgEcon
Sedjo, Roger A..
The responsibility for protecting U.S. agriculture from pests and diseases is assigned by the Federal Plant Pest Act (FPPA) to the Animal and Plant Health Inspection Service (APHIS) of the Department of Agriculture. The Plant Protection Act (Title 7 U.S.C. Sections 7701 et seq.) gives Aphis statutory authority over genetically modified organisms (GMO), in effect assigning to APHIS a related responsibility of determining whether a genetically altered plant, crop, or tree is likely to pose unacceptable risks to the environment. Although APHIS has considerable experience with crop plants, it has only limited experience with trees. Yet the possible benefits of applying genetic engineering to trees are substantial and include industrial wood production and...
Tipo: Working or Discussion Paper Palavras-chave: Forestry; Biotechnology; Transgenic; Tree plantations; Timber supply; Genes; GMOs; Industrial wood; Economics; Regulations; Costs; Benefits; Conservation; Resource /Energy Economics and Policy; Q21; Q23; Q16; O32; L73.
Ano: 2004 URL: http://purl.umn.edu/10629
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Valuing Transgenic Cotton Technologies Using a Risk/Return Framework AgEcon
Bryant, Kelly J.; Reeves, Jeanne M.; Nichols, Robert L.; Greene, Jeremy K.; Tingle, Christopher H.; Studebaker, Glenn E.; Bourland, Fred M.; Capps, Charles D.; Groves, Frank E..
Stochastic Efficiency with Respect to a Function (SERF) is used to rank transgenic cotton technology groups and place an upper and lower bound on their value. Yield and production data from replicated plot experiments are used to build cumulative distribution functions of returns for nontransgenic, Roundup Ready, Bollgard, and stacked gene cotton cultivars. Analysis of Arkansas data indicated that the stacked gene and Roundup Ready technologies would be preferred by a large number of risk neutral and risk averse producers as long as the costs of the technology and seed are below the lower bounds calculated in this manuscript.
Tipo: Journal Article Palavras-chave: Cotton; Financial risk; Market value; SERF; Transgenic; Agribusiness; Crop Production/Industries; Risk and Uncertainty; Q12; Q16.
Ano: 2008 URL: http://purl.umn.edu/47257
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Welfare Measures and Mandatory Regulation for Transgenic Food in the European Union: A Theoretical Framework for the Analysis AgEcon
Diez, Maria Del Carmen Fernandez.
This paper presents an analytical framework for studying the impact of mandatory labelling regulation for transgenic food. We compare Genetically Modified (GM) and conventional crop markets and identify gains for food processors prior to mandatory labelling and losses after this measure for the GM market. Nevertheless, food processors could obtain gains for conventional products after market disgregation. Finally, consumers will be worse off both for conventional and GM foods unless qualities other than changes to prices are considered.
Tipo: Conference Paper or Presentation Palavras-chave: Welfare; Mandatory labelling; Transgenic; Genetically modified organism; European Union policy; Agricultural and Food Policy; Q18; K32; D62.
Ano: 2005 URL: http://purl.umn.edu/24472
Registros recuperados: 21
Primeira ... 12 ... Última
 

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