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Registros recuperados: 24
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A low-cost apparatus for transforming Drosophila and detecting green fluorescent protein (GFP) genetic markers Genet. Mol. Biol.
Deprá,Maríndia; Sepel,Lenira Maria Nunes; Loreto,Élgion Lucio da Silva.
We describe the transformation of white mutant Drosophila simulans with a piggyBac transposon vector and a green fluorescent marker (GFP) and show how to construct inexpensive micro-manipulation and epifluorescence equipment for use in transposon-mediated germ-line transformation. Although the number of G0 adult flies (16) obtained in relation to the number of injected eggs was very low (12.5%) it was comparable to the proportion described by other authors and can be considered as a good rate of transformation.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Transgenic Drosophila; PiggyBac; GFP; Transposable elements.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000100012
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A re-annotation of the Anopheles darlingi mobilome Genet. Mol. Biol.
Diesel,Jose Francisco; Ortiz,Mauro F.; Marinotti,Osvaldo; Vasconcelos,Ana Tereza R.; Loreto,Elgion L. S..
Abstract The mobilome, portion of the genome composed of transposable elements (TEs), of Anopheles darlingi was described together with the genome of this species. Here, this mobilome was revised using similarity and de novo search approaches. A total of 5.6% of the A. darlingi genome is derived of TEs. Class I gypsy and copia were the most abundant superfamilies, corresponding to 22.36% of the mobilome. Non-LTR elements of the R1 and Jockey superfamilies account for 11% of the TEs. Among Class II TEs, the mariner superfamily is the most abundant (16.01%). Approximately 87% of the A. darlingi mobilome consist of short, truncated and/or degenerated copies of TEs. Only three retrotransposons, two belonging to gypsy and one to copia superfamilies, are...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Transposable elements; Transposons; Mosquitoes; Evolvability..
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000100125
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A transposable element annotation pipeline and expression analysis reveal potentially active elements in the microalga Tisochrysis lutea ArchiMer
Berthelier, Jeremy; Casse, Nathalie; Daccord, Nicolas; Jamilloux, Veronique; Saint-jean, Bruno; Carrier, Gregory.
Background Transposable elements (TEs) are mobile DNA sequences known as drivers of genome evolution. Their impacts have been widely studied in animals, plants and insects, but little is known about them in microalgae. In a previous study, we compared the genetic polymorphisms between strains of the haptophyte microalga Tisochrysis lutea and suggested the involvement of active autonomous TEs in their genome evolution. Results To identify potentially autonomous TEs, we designed a pipeline named PiRATE (Pipeline to Retrieve and Annotate Transposable Elements, download: https://doi.org/10.17882/51795), and conducted an accurate TE annotation on a new genome assembly of T. lutea. PiRATE is composed of detection, classification and annotation steps. Its...
Tipo: Text Palavras-chave: Transposable elements; Genome assembly; Pipeline; Tool; Annotation; Algae; Haptophyte; Tisochrysis lutea.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00441/55250/56732.pdf
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Abundance, distribution and potential impact of transposable elements in the genome of Mycosphaerella fijiensis. Repositório Alice
SANTANA, M. F.; SILVA, J. C. F.; BATISTA, A. D.; RIBEIRO, L. E.; SILVA, G. F. da; ARAÚJO, E. F. de; QUEIROZ, M. V. de.
Mycosphaerella fijiensis is a ascomycete that causes Black Sigatoka in bananas. Recently, the M. fijiensis genome was sequenced. Repetitive sequences are ubiquitous components of fungal genomes. In most genomic analyses, repetitive sequences are associated withtransposable elements (TEs). TEs are dispersed repetitive DNA sequences found in a host genome. These elements have the ability to move from one location to another within the genome, and their insertion can cause a wide spectrum of mutations in their hosts. Some of the deleterious effects of TEs may be due to ectopic recombination among TEs of the same family. In addition, some transposons are physically linked to genes and can control their expression. To prevent possible damage caused by the...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Mycosphaerella fijiensis; Transposable elements; RIP; Genome.
Ano: 2012 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/946093
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Analysis of putative transposable element-induced mutants in sorghum. Repositório Alice
CARVALHO, C.H.S.; ZEHR, U.B.; CHOPRA, S.; AXTELL, J.D..
2002
Tipo: Artigo em anais de congresso (ALICE) Palavras-chave: Sorghum; Cs1; Transposable elements; Gene tagging.
Ano: 2002 URL: http://www.alice.cnptia.embrapa.br/handle/doc/485685
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Boty-II, a novel LTR retrotransposon in Botrytis cinerea B05.10 revealed by genomic sequence Electron. J. Biotechnol.
Zhao,Ming; Zhou,Jin yan; Li,Zhi dong; Song,Wei wei; Tan,You jiu; Tan,Hong.
Botrytis cinerea is a necrotrophic pathogen causing pre- and post-harvest diseases in at least 235 plant species. It manifests extraordinary genotype and phenotype variation. One of the causes of this variation is transposable elements. Two transposable elements have been discovered in this fungus, the retrotransposon (Boty), and the transposon (Flipper). In this work, two complete (Boty-II-76 and Boty-II-103) and two partial (Boty-II-95 and Boty-II-141) long terminal repeat (LTR) retrotransposons were identified by an in silico genomic sequence analysis. Boty-II-76 and Boty-II-103 contain 6439 bp nucleotides with a pair of LTRs at both ends, and an internal deduced pol gene encoding a polyprotein with reverse transcriptase and DDE integrase domains. They...
Tipo: Journal article Palavras-chave: Botrytis cinerea; LTR retrotransposons; Molecular marker; Plant pathogen; Population diversity; Transposable elements.
Ano: 2009 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582009000300002
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Chromosomal distribution and evolution of abundant retrotransposons in plants: gypsy elements in diploid and polyploid Brachiaria forage grasses. Repositório Alice
SANTOS, F. C.; VALLE, C. B. do; CHIARI, L..
2015
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Centromeres; Retrotransposons; FISH; In situ hybridization; Metaviridae; Grasses; Genomics; Genome organization; Transposons; Transposable elements; Genetics; Repetitive DNA; Chromosomes.
Ano: 2015 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1040145
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Chromosomal distribution of the retroelements Rex 1, Rex 3 and Rex 6 in species of the genus Harttia and Hypostomus (Siluriformes: Loricariidae) Neotropical Ichthyology
Traldi,Josiane B.; Lui,Roberto L.; Martinez,Juliana de F.; Vicari,Marcelo R.; Nogaroto,Viviane; Moreira Filho,Orlando; Blanco,Daniel R..
ABSTRACT The transposable elements (TE) have been widely applied as physical chromosome markers. However, in Loricariidae there are few physical mapping analyses of these elements. Considering the importance of transposable elements for chromosomal evolution and genome organization, this study conducted the physical chromosome mapping of retroelements (RTEs) Rex1, Rex3 and Rex6 in seven species of the genus Harttia and four species of the genus Hypostomus, aiming to better understand the organization and dynamics of genomes of Loricariidae species. The results showed an intense accumulation of RTEs Rex1, Rex3 and Rex6 and dispersed distribution in heterochromatic and euchromatic regions in the genomes of the species studied here. The presence of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Armored catfish; Fluorescence in situ hybridization; Hypostominae; Loricariinae; Transposable elements.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252019000200214
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DNA methylation of the endogenous retrovirus Fematrin-1 in fetal placenta is associated with survival rate of cloned calves. Repositório Alice
SILVEIRA, M. M.; VARGAS, L. N.; BAYÃO, H. X. S.; SCHUMANN, N. A. B.; CAETANO, A. R.; RUMPF, R.; FRANCO, M. M..
bitstream/item/203972/1/1-s2.0-S0143400419306708-main.pdf
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Transposable elements; Retrotransposon; Envelope protein; Placental morphogenesis; Cloning; Epigenetics.
Ano: 2019 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1113684
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Drosophila relics hobo and hobo-MITEs transposons as raw material for new regulatory networks Genet. Mol. Biol.
Loreto,Elgion L.S.; Deprá,Maríndia; Diesel,José F.; Panzera,Yanina; Valente-Gaiesky,Vera Lucia S..
Abstract Hypermutable strains of Drosophila simulans have been studied for 20 years. Several mutants were isolated and characterized, some of which had phenotypes associated with alteration in development; for example, showing ectopic legs with eyes being expressed in place of antennae. The causal agent of this hypermutability is a non-autonomous hobo-related sequence (hoboVA). Around 100 mobilizable copies of this element are present in the D. simulans genome, and these are likely mobilized by the autonomous and canonical hobo element. We have shown that hoboVA has transcription factor binding sites for the developmental genes, hunchback and even-skipped, and that this transposon is expressed in embryos, following the patterns of these genes. We suggest...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Hobo; Transposable elements; Cis-regulatory sequences; Drosophila.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000200198
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Evidence of ectopic recombination and a repeat-induced point (RIP) mutation in the genome of Sclerotinia sclerotiorum, the agent responsible for white mold Genet. Mol. Biol.
Goldfarb,Míriam; Santana,Mateus Ferreira; Salomão,Tânia Maria Fernandes; Queiroz,Marisa Vieira de; Barros,Everaldo Gonçalves de.
Abstract Two retrotransposons from the superfamilies Copia and Gypsy named as Copia-LTR_SS and Gypsy-LTR_SS, respectively, were identified in the genomic bank of Sclerotinia sclerotiorum. These transposable elements (TEs) contained direct and preserved long terminal repeats (LTR). Domains related to codified regions for gag protein, integrase, reverse transcriptase and RNAse H were identified in Copia-LTR_SS, whereas in Gypsy-LTR_SS only domains for gag, reverse transcriptase and RNAse H were found. The abundance of identified LTR-Solo suggested possible genetic recombination events in the S. sclerotiorum genome. Furthermore, alignment of the sequences for LTR elements from each superfamily suggested the presence of a RIP (repeat-induced point mutation)...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Phytopathogens; Retrotransposons; Transposable elements.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000300426
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Evolutionary history of the Tip100 transposon in the genus Ipomoea Genet. Mol. Biol.
Christoff,Ana-Paula; Loreto,Elgion L.S.; Sepel,Lenira M.N..
Tip100 is an Ac-like transposable element that belongs to the hAT superfamily. First discovered in Ipomoea purpurea (common morning glory), it was classified as an autonomous element capable of movement within the genome. As Tip100 data were already available in databases, the sequences of related elements in ten additional species of Ipomoea and five commercial varieties were isolated and analyzed. Evolutionary analysis based on sequence diversity in nuclear ribosomal Internal Transcribed Spacers (ITS), was also applied to compare the evolution of these elements with that of Tip100 in the Ipomoea genus. Tip100 sequences were found in I. purpurea, I. nil, I. indica and I. alba, all of which showed high levels of similarity. The results of phylogenetic...
Tipo: Info:eu-repo/semantics/article Palavras-chave: HAT; Transposable elements; Ac-Ds; Ipomoea; Genome evolution; ITS.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000300013
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Functional characterization of sugarcane mustang domesticated transposases and comparative diversity in sugarcane, rice, maize and sorghum Genet. Mol. Biol.
Kajihara,Daniela; Godoy,Fabiana de; Hamaji,Thais Alves; Blanco,Silvia Regina; Van Sluys,Marie-Anne; Rossi,Magdalena.
Transposable elements (TEs) account for a large portion of plant genomes, particularly in grasses, in which they correspond to 50%-80% of the genomic content. TEs have recently been shown to be a source of new genes and new regulatory networks. The most striking contribution of TEs is referred as “molecular domestication”, by which the element coding sequence loses its movement capacity and acquires cellular function. Recently, domesticated transposases known as mustang and derived from the Mutator element have been described in sugarcane. In order to improve our understanding of the function of these proteins, we identified mustang genes from Sorghum bicolor and Zea mays and performed a phenetic analysis to assess the diversity and evolutionary history of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Domesticated tranposases; Saccharum sp.; Sorghum bicolor; Transposable elements; Zea mays.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000400014
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Genetic transformation of Drosophila willistoni using piggyBac transposon and GFP BABT
Finokiet,Manuela; Goni,Beatriz; Loreto,Élgion Lúcio Silva.
Studies were carried out on the use of piggyBac transposable element as vector and the green fluorescent protein (EGFP) from the jellyfish, Aquorea victoria, as a genetic marker for the transformation of Drosophila willistoni. Preblastoderm embryos of D. willistoni white mutant were microinjected with a plasmid containing the EGFP marker and the piggyBac ITRs, together with a helper plasmid containing the piggyBac transposase placed under the control of the D. melanogaster hsp70 promoter. G0 adults transformants were recovered at a frequency of approximately 67%. Expression of EGFP in larvae, pupae and adults was observed up to the third generation, suggesting that this transposon was not stable in D. willistoni. Transformed individuals displayed high...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Transposable elements; Insect transgenesis; Germ-line transformation; Fluorescent protein marker; Microinjection.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000100013
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Genome assembly of the Pink Ipê (Handroanthus impetiginosus, Bignoniaceae), a highly valued, ecologically keystone Neotropical timber forest tree. Repositório Alice
SILVA-JUNIOR, O. B.; GRATTAPAGLIA, D.; NOVAES, E.; COLLEVATTI, R. G..
bitstream/item/181067/1/gix125.pdf
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Heterozygous genome; RNA-Seq; Quinoids; Transposable elements; Bignoniaceae.
Ano: 2018 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1085131
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Geographic polymorphism of P element in populations of Drosophila sturtevanti Genet. Mol. Biol.
Almeida,Luciane M. de; Langeani,Francisco; Carareto,Claudia M.A..
The aim of this report was to detect full-sized P element sequences in eight strains of Drosophila sturtevanti populations from distant geographic regions and to assess the structural geographic variation among P element sequences. PCR analysis confirmed the presence of a putative complete P element in all strains. Southern blot analysis indicated bands shared by all strains, and bands restricted to geographically related strains. Parsimony analysis corroborated the hybridization pattern that reflected the geographic relationships.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Canonical P elements; Drosophila; P element polymorphism; Saltans group; Transposable elements.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000200010
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Gonadal hybrid dysgenesis in Drosophila sturtevanti (Diptera, Drosophilidae) Iheringia, Sér. Zool.
Almeida,Luciane M. de; Carareto,Claudia M. A..
The occurrence of hybrid dysgenesis was investigated in Drosophila sturtevanti Duda, 1927 using diagnostic crosses similar to those used for induction of dysgenics traits in D. melanogaster. Reciprocal test crosses were made, at 27° C, between an old laboratory strain of D. sturtevanti (COL, from Colombia), assumed to be an M'-like strain, and eight freshly collected strains from several natural populations. The gonadal dysgenesis indices were under 10% in most of crosses, except in hybrids of COL with I27, a strain from Minas Gerais (Brazil), in which the index values were moderate in both directions of crosses (25.71 and 12.87). The smallest productivity was also observed in hybrids of females COL mated to I27 males. No causal relationship between the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Hybrid sterility; P element; I element; Hobo element; Transposable elements.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0073-47212002000200007
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Highly efficient pipeline to perform integrated analysis of transposable elements and tandem repeats in complex genomes. Repositório Alice
BRITO, L. S. de.; FERREIRA FILHO, J. A.; SOUZA, M. S.; FORMIGHIERI, E. F..
2016
Tipo: Artigo em anais de congresso (ALICE) Palavras-chave: Pipeline; Transposable elements; Microsatellites; Bioinformatics; Perl; Elementos transponíveis; Microsatélites; Bioinformática.
Ano: 2016 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1057082
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Identification and characterization of spontaneous mutants in the 'Sunki' mandarin group. Repositório Alice
SANTANA, D.D.S.; MILORI, D. M. B. P.; GAIOTO, F. A.; SOARES FILHO, W. dos S.; GESTEIRA, A. da S..
Mandarins of the 'Sunki' [Citrus sunki (Hayata) hort. ex Tanaka] group have great potential for diversification of citrus rootstock in Brazil, with emphasis on the 'Tropical' selection of 'Sunki', obtained by Embrapa Cassava and Fruits, a natural mutation of nucellar origin, from a common clone of this mandarin.
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Citrus; Mutations; Transposable elements; Molecular markers; Genetic breeding.
Ano: 2011 URL: http://www.alice.cnptia.embrapa.br/handle/doc/923269
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New insights into Phakopsora pachyrhizi infection based on transcriptome analysis in planta Genet. Mol. Biol.
Rincão,Michelle Pires; Carvalho,Mayra Costa da Cruz Gallo de; Nascimento,Leandro Costa; Lopes-Caitar,Valéria S.; Carvalho,Kenia de; Darben,Luana M.; Yokoyama,Alessandra; Carazzolle,Marcelo Falsarella; Abdelnoor,Ricardo Vilela; Marcelino-Guimarães,Francismar Correa.
Abstract Asian soybean rust (ASR) is one of the most destructive diseases affecting soybeans. The causative agent of ASR, the fungus Phakopsora pachyrhizi, presents characteristics that make it difficult to study in vitro, limiting our knowledge of plant-pathogen dynamics. Therefore, this work used leaf lesion laser microdissection associated with deep sequencing to determine the pathogen transcriptome during compatible and incompatible interactions with soybean. The 36,350 generated unisequences provided an overview of the main genes and biological pathways that were active in the fungus during the infection cycle. We also identified the most expressed transcripts, including sequences similar to other fungal virulence and signaling proteins. Enriched P....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Asian soybean rust; Multigenic families; Transposable elements.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000400671
Registros recuperados: 24
Primeira ... 12 ... Última
 

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