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Lactic acid bacteria increase the resistance of turbot larvae, Scophthalmus maximus, against pathogenic vibrio ArchiMer
Gatesoupe, Fj.
Characteristics of Lactic Acid Bacteria (LAB) isolated from rotifers, Brachionus plicatilis, resembled those of Lactobacillus plantarum or Carnobacterium sp. It was investigated whether the artificial maintenance of a high concentration of LAB in rotifers might increase their dietary value for turbot larvae, particularly when the fish were infected with pathogenic Vibrio sp. One LAB strain was cultivated, then introduced daily into the enrichment medium of rotifers. In this way, LAB were retrieved in large amounts in turbot, and a significant limitation of larval mortality rate was observed when turbot were challenged with pathogenic vibrio at day 9. The inoculum concentration of LAB had a decisive effect on survival rate, and the optimum was between 10(7)...
Tipo: Text Palavras-chave: LACTIC ACID BACTERIA; VIBRIO; PATHOGEN; ROTIFER; EXPERIMENTAL INFECTION; LARVAL FISH CULTURE; TURBOT; PLEURONECTIFORMES.
Ano: 1994 URL: http://archimer.ifremer.fr/doc/00065/17672/15200.pdf
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Recombinant expression and anti-microbial activity of anti-lipopolysaccharide factor (ALF) from the black tiger shrimp Penaeus monodon Inra
Somboonwiwat, K.; Marcos, M.; Tassanakajon, A.; Klinbunga, S.; Aumelas, A.; Romestand, B.; Gueguen, Y.; Boze, H.; Moulin, G.; Bachère, E..
Anti-lipopolysaccharide factors (ALFs), originally characterized from horseshoe crabs, have been recently identified from hemocytes of the black tiger shrimp, Penaeus monodon, by a genomic approach. In order to characterize the properties and biological activities of this immune effector in shrimp, ALFPm3, the most abundant isoform found in P. monodon, was expressed in the yeast Pichia pastoris. Large-scale production in fermentor provided 262 mg/l of recombinant ALFPm3 which was purified to homogeneity by single chromatography step on expanded-bed Streamline SP6XL. The rALFPm3 was further characterized in terms of N-terminal sequencing and mass spectrometry. Anti-microbial assays demonstrated that rALFPm3 has a broad spectrum of anti-fungal properties...
Tipo: Journal Article Palavras-chave: SHRIMP; CRUSTACEAN; ANTI-LIPOPOLYSACCHARIDE FACTOR; LPS-BINDING; ANTI6mICROBIAL ACTIVITY; VIBRIO; HEMOCYTE.
Ano: 2005 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20092e49f543&uri=/notices/prodinra1/2010/10/
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