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Amplification of tlh gene in other Vibrionaceae specie by specie-specific multiplex PCR of Vibrio parahaemolyticus Electron. J. Biotechnol.
Yáñez,Romina; Bastías,Roberto; Higuera,Gastón; Salgado,Oscar; Katharios,Pantelis; Romero,Jaime; Espejo,Romilio; García,Katherine.
Background The surveillance of Vibrio parahaemolyticus in the Chilean coast has been mainly performed by multiplex PCR amplification of three different hemolysin genes, which are specie-specific virulence factors. These genes are also employed in the determination of V. parahaemolyticus pathogenic load in seafood and for characterization of pathogenic strains associated to diarrhea cases in human. During environmental surveillance that we performed every summer, we occasionally observed a thermolabile hemolysin (tlh) PCR product of a slightly smaller size than expected, which was coincident with low loads of V. parahaemolyticus in the environment. In order to understand this observation, we probed the specificity of tlh primers for the detection of V....
Tipo: Journal article Palavras-chave: Hemolysin; Multiplex PCR; Pathogen surveillance; Virulence factor; Vibrio parahaemolyticus.
Ano: 2015 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000600012
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Avaliação de parâmetros físicos e químicos e pesquisa de Vibrio parahaemolyticus em águas do estuário do rio Anil (São Luís, Estado do Maranhão) - DOI: 10.4025/actascibiolsci.v25i2.1978 Biological Sciences
Serra, Carmen Lucia Martins; UFMA; Cavalcante, Paulo Roberto Saraiva; UFMA; Alves, Lucia Maria Coelho; UFMA; Nascimento, Adenilde Ribeiro; UFMA; Diniz, Silvia Cristina Costa de Santana de; UFMA.
Avaliação dos parâmetros físico-químicos e isolamento de Vibrio parahaemolyticus em águas do estuário do rio Anil (São Luís-MA, Brasil), em bancos naturais de moluscos. Foi utilizada a técnica do Número Mais Provável (NMP) com enriquecimento e subseqüente isolamento em agar tiossulfato-citrato-sacarose-sais-biliares (TCBS). Adicionalmente, foram monitorados “in situ” alguns parâmetros físico-químicos. As determinações para o pH variaram (7,25 a 7,90), O2 (0,20 a 1,2mg/L), T°C (29 a 32°C), S‰ (5 a 28,0 UPS), NH4+ (0,04 a 3,41mg/L), NO2- (0,10 a 1,91mg/L), PO43- (0,03 a 4,88mg/L). Em 56,1% na estação I e 55,4% na estação II, apresentaram positividade, para V. parahaemolyticus, com níveis entre 1,1x10 e > 1,1x105NMP/mL. Os resultados exigem uma atenção...
Palavras-chave: 2.01.00.00-0 Biologia Geral parâmetros físicos e químicos; Vibrio parahaemolyticus; Estuário; Nutrientes; Análises de água 2.01.00.00-0 Biologia Geral.
Ano: 2003 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/1978
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Bactéries pathogènes indigènes des eaux estuariennes: cas des vibrios. Revue bibliographique ArchiMer
Fouche, Dominique.
De nombreuses bactéries, peuplant habituellement les zones estuariennes, se révèlent être de plus en plus fréquemment des agents de maladies dont les vecteurs principaux sont les coquillages et leur consommation par l'homme. Le présent travail synthétise l'état des connaissances sur les vibrios, en particulier Vibrio cholerae, Vibrio parahaemolyticus et Vibrio vulnificus, bactéries pour lesquelles il existe une  abondante littérature. L'écologie, le pouvoir pathogène et les milieux de culture les plus couramment utilisés en vue de leur détection sont présentés. Enfin, leur interaction avec les mollusques bivalves, leur survie dans ceux-ci et le problème de l'épuration conséquente de ces derniers sont abordés.
Tipo: Text Palavras-chave: Vibrio cholerae; Vibrio parahaemolyticus; Vibrio vulnificus; Bactéries indigènes; Estuaires; Bivalves; Purification.
Ano: 1993 URL: http://archimer.ifremer.fr/doc/00408/51929/52554.pdf
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Epidemiological investigation of a foodborne outbreak in Spain associated with U.S. West Coast genotypes of Vibrio parahaemolyticus ArchiMer
Martinez-urtaza, Jaime; Powell, Andy; Jansa, Josep; Castro Rey, Jose Luis; Paz Montero, Oscar; Garcia Campello, Marta; Zamora Lopez, Ma Jose; Pousa, Anxela; Faraldo Valles, Ma Jose; Trinanes, Joaquin; Hervio-heath, Dominique; Keay, William; Bayley, Amanda; Hartnell, Rachel; Baker-austin, Craig.
We describe an outbreak of seafood-associated Vibrio parahaemolyticus in Galicia, Spain in on 18th of August 2012 affecting 100 of the 114 passengers travelling on a food banquet cruise boat. Epidemiological information from 65 people was available from follow-on interviews, of which 51 cases showed symptoms of illness. The food items identified through the questionnaires as the most probable source of the infections was shrimp. This product was unique in showing a statistically significant and the highest OR with a value of 7.59 (1.52–37.71). All the nine strains isolated from stool samples were identified as V. parahaemolyticus, seven were positive for both virulence markers tdh and trh, a single strain was positive for trh only and the remaining strain...
Tipo: Text Palavras-chave: Seafood; Tdh; Trh; Vibrio parahaemolyticus; PNW clone; ST 36.
Ano: 2016 URL: http://archimer.ifremer.fr/doc/00311/42181/41482.pdf
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Microbial contamination of sand from major beaches in Fortaleza, Ceará State, Brazil BJM
Vieira,Regine Helena Silva dos Fernandes; Rodrigues,Dália dos Prazeres; Menezes,Everardo A.; Evangelista,Norma Suely de Santana; Reis,Eliane M.F. dos; Barreto,Leopoldo Melo; Gonçalves,Flávia Araújo.
The presence of faecal contamination and pathogenic microorganisms in samples of dry and wet sand collected from three major beaches in Fortaleza, Ceará State, Brazil: (Praia do Mucuripe, Praia do Futuro and Praia do Caça e Pesca), during the period of May 1999 to January 2000 was evaluated. Praia do Caça e Pesca had the highest incidence of E. coli in dry sand (56%) followed by Praia do Mucuripe (28%) and Praia do Futuro (16%). In wet sand, results were 48%, 28% and 24% for Praia do Caça e Pesca, Praia do Futuro and Praia do Mucuripe, respectively. Only two samples from Praia do Futuro, one from dry sand and another one from wet sand, were positive for Salmonella. V. parahaemolyticus was isolated from four samples from Praia do Caça e Pesca (two from...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Sand contamination; Yeasts; Faecal coliforms; Vibrio parahaemolyticus; Salmonella.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000200001
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MRNA detection by reverse transcription-PCR for monitoring viability and potential virulence in a pathogenic strain of Vibrio parahaemolyticus in viable but nonculturable state ArchiMer
Coutard, Francois; Pommepuy, Monique; Loaec, Solen; Hervio Heath, Dominique.
Aims: This work investigates the maintenance of viability and potential virulence of Vibrio parahaemolyticus in a viable but nonculturable population (VBNC) state by reverse transcription-polymerase chain reaction (RT-PCR). Methods and Results: Housekeeping genes, 16S-23S rDNA and rpoS, as well as virulence genes, tdh1 and tdh2, were selected and detected by PCR in a pathogenic strain of V. parahaemolyticus (Vp4). Their expression was then studied by RT-PCR in V. parahaemolyticus Vp4 cultivated in rich medium at 37degreesC. The 16S-23S rDNA and rpoS, tdh1, tdh2 genes were transcripted at the mid-logarithmic, stationary and late stationary phases, corresponding to various physiological states. The expression of these genes was also studied by RT-PCR in a...
Tipo: Text Palavras-chave: Vibrio parahaemolyticus; Viable but nonculturable state; Tdh2; Tdh1; RT PCR; RpoS; Environment; 16S 23S rDNA.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/publication-1098.pdf
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Recovery in culture of viable but nonculturable Vibrio parahaemolyticus: regrowth or resuscitation? ArchiMer
Coutard, Francois; Crassous, Philippe; Droguet, Michael; Gobin, Eric; Colwell, Rita; Pommepuy, Monique; Hervio Heath, Dominique.
The objective of this study was to explore the recovery of culturability of viable but nonculturable ( VBNC) Vibrio parahaemolyticus after temperature upshift and to determine whether regrowth or resuscitation occurred. A clinical strain of V. parahaemolyticus Vp5 was rendered VBNC by exposure to artificial seawater ( ASW) at 4 degrees C. Aliquots of the ASW suspension of cells ( 0.1, 1 and 10 ml) were subjected to increased temperatures of 20 degrees C and 37 degrees C. Culturability of the cells in the aliquots was monitored for colony formation on a rich medium and changes in morphology were measured by scanning ( SEM) and transmission ( TEM) electron microscopy. Samples of VBNC cells were fixed and examined by SEM, revealing a heterogeneous population...
Tipo: Text Palavras-chave: Transmission electron microscopy; Scanning electron microscopy; Resuscitation; Regrowth; Vibrio parahaemolyticus; VBNC state.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-3117.pdf
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The use of API 20E for the identification of <i>Vibrio anguillarum</i> and <i>V. ordalii</i> OMA
Grisez, L.; Ceusters, R.; Ollevier, F.P..
Sixty-eight strains of <i>Vibrio anguillarum</i>, five of <i>V. ordalii</i> and the type strains of <i>V. alginolyticus, V. carchariae, V. damsela</i> and <i>V. parahaemolyticus</i> were compared using the API 20E gallery. Within the <i>V. anguillarum</i> strains, distinct groups could be separated mainly on the basis of their reaction on indole production and the fermentation of amygdalin and arabinose. <i>Vibrio ordalii</i>, the former <i>V. anguillarum</i> biotope 2, could easily be separated from <i>V. anguillarum</i> and from the other fish pathogenic <i>Vibrio</i> spp.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacterial diseases; Disease control; Disease detection; Fish diseases; Identification; Vibrio carchariae; Vibrio ordalii; Vibrio alginolyticus; Vibrio anguillarum; Vibrio damsela; Vibrio parahaemolyticus.
Ano: 1991 URL: http://www.vliz.be/nl/open-marien-archief?module=ref&refid=2859
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