Sabiia Seb
        Busca avançada

Botão Atualizar

Botão Atualizar

Registro completo
Provedor de dados:  Agronomy
País:  Brazil
Título:  Mutation of Trp-574-Leu ALS gene confers resistance of radish biotypes to iodosulfuron and imazethapyr herbicides
Autores:  Cechin, Joanei
Vargas, Leandro
Agostinetto, Dirceu
Lamego, Fabiane Pinto
Mariani, Franciele
Dal Magro, Taísa
Data:  2017-05-17
Ano:  2017
Palavras-chave: Raphanus sativus
Mechanism of resistence
ALS enzyme activity
Gene mutation
Herbologia e Resistência de plantas daninhas
Resumo:  Acetolactate synthase inhibitors are the main group of herbicides used in winter crops in Southern Brazil where their intensive use has selected for herbicide-resistant biotypes of radish. The resistance affects the efficacy of herbicides, and identifying the resistance mechanism involved is important for defining management strategies. The aim of this study was to elucidate the resistance mechanism of radish biotypes by quantifying the enzyme activity, ALS gene sequencing and evaluating the response of biotypes to iodosulfuron and imazethapyr herbicide application after treatment with a cytochrome P450 monooxygenase inhibitor. The susceptible (B1) and resistant (B4 and B13) biotypes were from wheat fields in the Northwest of Rio Grande do Sul State. The results demonstrated that the enzyme affinity for the substrate (KM) was not affected in biotypes B4 and B13 but that the Vmax of the resistant biotypes was higher than that of biotype B1. The resistant biotypes showed no differential metabolic response to iodosulfuron and imazethapyr herbicides when inhibited by malathion and piperonyl butoxide. However, gene sequencing of ALS showed a mutation at position 574, with an amino acid substitution of tryptophan for leucine (Trp-574-Leu) in resistant biotypes. 
Tipo:  Info:eu-repo/semantics/article
Idioma:  Inglês

Editor:  Universidade Estadual de Maringá
Formato:  application/pdf
Fonte:  Acta Scientiarum. Agronomy; v. 39, n. 3 (2017); 299-306

Acta Scientiarum. Agronomy; v. 39, n. 3 (2017); 299-306


Direitos:  Direitos autorais 2017 Acta Scientiarum. Agronomy

Empresa Brasileira de Pesquisa Agropecuária - Embrapa
Todos os direitos reservados, conforme Lei n° 9.610
Política de Privacidade
Área restrita

Parque Estação Biológica - PqEB s/n°
Brasília, DF - Brasil - CEP 70770-901
Fone: (61) 3448-4433 - Fax: (61) 3448-4890 / 3448-4891 SAC:

Valid HTML 4.01 Transitional