| Registro completo |
| Provedor de dados: |
Agronomy
|
| País: |
Brazil
|
| Título: |
Non-destructive analysis of photosynthetic pigments in cotton plants - doi: 10.4025/actasciagron.v33i4.10926
Non-destructive analysis of photosynthetic pigments in cotton plants - doi: 10.4025/actasciagron.v33i4.10926
|
| Autores: |
Brito, Giovani Greigh; Empresa Brasileira de Pesquisa Agropecuária
Sofiatti, Valdinei; Empresa Brasileira de Pesquisa Agropecuária
Brandão, Ziany Neiva; Empresa Brasileira de Pesquisa Agropecuária
Silva, Vivianny Belo; Empresa Brasileira de Pesquisa Agropecuária
Silva, Franklin Magnum; Empresa Brasileira de Pesquisa Agropecuária
Silva, Dalva Almeida; Empresa Brasileira de Pesquisa Agropecuária
|
| Data: |
2011-09-29
|
| Ano: |
2011
|
| Palavras-chave: |
Fisiologia de plantas cultivadas Gossypium hirsutum
Chlorophyll
Carotenoids
Cotton 5.01.03.06-7 Gossypium hirsutum
Chlorophyll
Carotenoids
Cotton
|
| Resumo: |
Analytical techniques used to extract chlorophyll from plant leaves are destructive and based on the use of organic solvents. This study proposes a non-destructive quantification of the photosynthetic pigment concentration in cotton leaves using two portable chlorophyll meters, the SPAD-502 and the CLOROFILOG 1030. After obtaining 200 leaf discs, each with an area of 113 mm2, the greening rate in each disc was determined by the average of five readings from both meters. Immediately after measurement, 5 mL of dimethyl sulfoxide (DMSO) was added, and the samples were kept in a water bath at 70ºC for 30 min. After cooling, 3 mL of the liquid extract was used for analyses by spectrophotometry at 470, 646 and 663 nm. Mathematical models were adjusted from analytical results using the reading index obtained from both devices to predict the contents of chlorophyll a, chlorophyll b, total chlorophyll and carotenoids. Based on these results, it was concluded that both portable chlorophyll meters are an effective way to estimate the concentration of photosynthetic pigments in cotton leaves, thus saving time, space and the resources that are often required for these analyses.
Analytical techniques used to extract chlorophyll from plant leaves are destructive and based on the use of organic solvents. This study proposes a non-destructive quantification of the photosynthetic pigment concentration in cotton leaves using two portable chlorophyll meters, the SPAD-502 and the CLOROFILOG 1030. After obtaining 200 leaf discs, each with an area of 113 mm2, the greening rate in each disc was determined by the average of five readings from both meters. Immediately after measurement, 5 mL of dimethyl sulfoxide (DMSO) was added, and the samples were kept in a water bath at 70ºC for 30 min. After cooling, 3 mL of the liquid extract was used for analyses by spectrophotometry at 470, 646 and 663 nm. Mathematical models were adjusted from analytical results using the reading index obtained from both devices to predict the contents of chlorophyll a, chlorophyll b, total chlorophyll and carotenoids. Based on these results, it was concluded that both portable chlorophyll meters are an effective way to estimate the concentration of photosynthetic pigments in cotton leaves, thus saving time, space and the resources that are often required for these analyses.
|
| Tipo: |
Pesquisa Empirica de Campo
|
| Idioma: |
Inglês
|
| Identificador: |
http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/10926
10.4025/actasciagron.v33i4.10926
|
| Editor: |
Universidade Estadual de Maringá
|
| Formato: |
application/pdf
application/pdf
|
| Fonte: |
Acta Scientiarum. Agronomy; v. 33, n. 4 (2011); 671-678
Acta Scientiarum. Agronomy; v. 33, n. 4 (2011); 671-678
|
| Direitos: |
DECLARATION OF ORIGINALITY AND CESSATION OF COPYRIGHTI Declare that current article is original and has not been submitted for publication, in part or in whole, to any other national or international journal. I also declare that once published in the Acta Scientiarum. Agronomy, a publication of the Universidade Estadual de Maringá, it will not be submitted by me or by any co-author to any other scientific communication mean. In my name and in the name of co-authors, I shall cede the copyright of the above mentioned article to the Universidade Estadual de Maringá and I declare that I know that the non-observance of this norm may make me liable for the penalties contemplated in the Law for the Protection of Authors’ Rights (Act 9609 of the 19th February 1998).
DECLARACIÓN DE ORIGINALIDAD Y CESIÓN DE DERECHOS DE AUTOR Declaro que el presente artículo es original, no fue sometido a la publicación en cualquier otro periódico nacional o internacional, sea en parte o en su totalidad. Declaro, aun, que una vez publicado en la revista Acta Scientiarum. Agronomy, editado por la Universidad Estadual de Maringá, éste jamás será sometido por mí o por cualquier uno de los demás coautores a cualquier otro periódico. A través de este instrumento, en mi nombre y en nombre de los demás coautores, por ventura existentes, cedo los derechos de autor del referido artículo a la Universidad Estadual de Maringá y declaro estar informado de que la no observancia de este compromiso someterá al infractor las sanciones y penas previstas en la Ley de Protección de Derechos de Autor (Nº9609, de 19/02/98).
DECLARAÇÃO DE ORIGINALIDADE E CESSÃO DE DIREITOS AUTORAISDeclaro que o presente artigo é original, não tendo sido submetido à publicação em qualquer outro periódico nacional ou internacional, quer seja em parte ou em sua totalidade. Declaro, ainda, que uma vez publicado na revista Acta Scientiarum. Agronomy, editada pela Universidade Estadual de Maringá, o mesmo jamais será submetido por mim ou por qualquer um dos demais co-autores a qualquer outro meio de divulgação científica. Através deste instrumento, em meu nome e em nome dos demais co-autores, porventura existentes, cedo os direitos autorais do referido artigo à Universidade Estadual de Maringá e declaro estar ciente de que a não observância deste compromisso submeterá o infrator a sanções e penas previstas na Lei de Proteção de Direitos Autorias (Nº9609, de 19/02/98).
|
|
