| Resumo: |
The objective of this work was to evaluate the alterations of antioxidant enzyme reserves and antioxidant enzymes during germination under water deficit in maize hybrids and to associate with seed vigor, determining the mechanisms related to tolerance for this stress. Two three-way maize hybrids were characterized by their vigor at different levels of water deficit induced by polyethylene glycol 6000. Next, the seeds were hydrated at different osmotic potentials (0.0, -0.3, and -0.9 MPa) and removed at different times to assess the levels of the total soluble protein, total soluble sugars, proline, starch, and antioxidant enzymes, such as superoxide dismutase, catalase and ascorbate peroxidase. The analysis of variance, Tukey test at 5% and principal component analysis (PCA) were used. The vigorous hybrid (HT1) was more efficient than the low vigor hybrid seeds (HT2) in mobilizing the total soluble protein during the initial stages of germination and the total soluble sugars before and after root protrusion under water deficit in addition to increasing the catalase activity at the different osmotic potentials that were assessed.
The objective of this work was to evaluate the alterations of antioxidant enzyme reserves and antioxidant enzymes during germination under water deficit in maize hybrids and to associate with seed vigor, determining the mechanisms related to tolerance for this stress. Two three-way maize hybrids were characterized by their vigor at different levels of water deficit induced by polyethylene glycol 6000. Next, the seeds were hydrated at different osmotic potentials (0.0, -0.3, and -0.9 MPa) and removed at different times to assess the levels of the total soluble protein, total soluble sugars, proline, starch, and antioxidant enzymes, such as superoxide dismutase, catalase and ascorbate peroxidase. The analysis of variance, Tukey test at 5% and principal component analysis (PCA) were used. The vigorous hybrid (HT1) was more efficient than the low vigor hybrid seeds (HT2) in mobilizing the total soluble protein during the initial stages of germination and the total soluble sugars before and after root protrusion under water deficit in addition to increasing the catalase activity at the different osmotic potentials that were assessed.
|
