| Registro completo |
| Provedor de dados: |
Agronomy
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| País: |
Brazil
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| Título: |
Peroxidase activity in roots of arracacha affected by pH and temperature - doi: 10.4025/actasciagron.v33i3.6264
Peroxidase activity in roots of arracacha affected by pH and temperature - doi: 10.4025/actasciagron.v33i3.6264
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| Autores: |
Menolli, Luciana Nunes; UFV
Finger, Fernando Luiz; UFV
Barbosa, Janaína Miranda; UFV
Correia, Teresa Drummond; UFV
Vieira, Luciana Marques; UFV
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| Data: |
2011-06-22
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| Ano: |
2011
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| Palavras-chave: |
5.01.00.00-9 Arracacia xanthorrhyza Bancroft
Oxidative enzyme
Inactivation 5.07.01.04-5 Arracacia xanthorrhyza Bancroft
Oxidative enzyme
Inactivation
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| Resumo: |
In this paper, roots of arracacha (Arracacia xanthorrhyza Bancroft) were stored at 5ºC to induce chilling injury symptoms and stress-related peroxidase activity. Later, peroxidase kinetic activity was determined in different pH and temperature conditions. For this, soluble crude extract was sequentially saturated with ammonium sulfate, obtaining a semi-purified enzyme solution used for the analysis. Activity of peroxidase induced by the chilling at 5oC was determined from pH 2.5 to 9.0 and at temperature ranging from 10 to 80oC. The peroxidase had higher activity when the reaction occurred between pH 5.5 and 6.0 and at temperature of 30oC. Complete inactivation of the activity was observed in pH 2.5 after 60 minutes of pre-incubation or at 60oC for 10 minutes or alternatively at 70oC after 5 minutes of pre-incubation. The enzyme is more susceptible to inactivation in acid than alkaline pHs or alternatively using heat treatment.
In this paper, roots of arracacha (Arracacia xanthorrhyza Bancroft) were stored at 5ºC to induce chilling injury symptoms and stress-related peroxidase activity. Later, peroxidase kinetic activity was determined in different pH and temperature conditions. For this, soluble crude extract was sequentially saturated with ammonium sulfate, obtaining a semi-purified enzyme solution used for the analysis. Activity of peroxidase induced by the chilling at 5oC was determined from pH 2.5 to 9.0 and at temperature ranging from 10 to 80oC. The peroxidase had higher activity when the reaction occurred between pH 5.5 and 6.0 and at temperature of 30oC. Complete inactivation of the activity was observed in pH 2.5 after 60 minutes of pre-incubation or at 60oC for 10 minutes or alternatively at 70oC after 5 minutes of pre-incubation. The enzyme is more susceptible to inactivation in acid than alkaline pHs or alternatively using heat treatment.
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| Idioma: |
Inglês
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| Identificador: |
http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/6264
10.4025/actasciagron.v33i3.6264
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| Editor: |
Universidade Estadual de Maringá
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| Formato: |
application/pdf
application/pdf
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| Fonte: |
Acta Scientiarum. Agronomy; v. 33, n. 3 (2011); 513-518
Acta Scientiarum. Agronomy; v. 33, n. 3 (2011); 513-518
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| Direitos: |
DECLARATION OF ORIGINALITY AND CESSATION OF COPYRIGHTI Declare that current article is original and has not been submitted for publication, in part or in whole, to any other national or international journal. I also declare that once published in the Acta Scientiarum. Agronomy, a publication of the Universidade Estadual de Maringá, it will not be submitted by me or by any co-author to any other scientific communication mean. In my name and in the name of co-authors, I shall cede the copyright of the above mentioned article to the Universidade Estadual de Maringá and I declare that I know that the non-observance of this norm may make me liable for the penalties contemplated in the Law for the Protection of Authors’ Rights (Act 9609 of the 19th February 1998).
DECLARACIÓN DE ORIGINALIDAD Y CESIÓN DE DERECHOS DE AUTOR Declaro que el presente artículo es original, no fue sometido a la publicación en cualquier otro periódico nacional o internacional, sea en parte o en su totalidad. Declaro, aun, que una vez publicado en la revista Acta Scientiarum. Agronomy, editado por la Universidad Estadual de Maringá, éste jamás será sometido por mí o por cualquier uno de los demás coautores a cualquier otro periódico. A través de este instrumento, en mi nombre y en nombre de los demás coautores, por ventura existentes, cedo los derechos de autor del referido artículo a la Universidad Estadual de Maringá y declaro estar informado de que la no observancia de este compromiso someterá al infractor las sanciones y penas previstas en la Ley de Protección de Derechos de Autor (Nº9609, de 19/02/98).
DECLARAÇÃO DE ORIGINALIDADE E CESSÃO DE DIREITOS AUTORAISDeclaro que o presente artigo é original, não tendo sido submetido à publicação em qualquer outro periódico nacional ou internacional, quer seja em parte ou em sua totalidade. Declaro, ainda, que uma vez publicado na revista Acta Scientiarum. Agronomy, editada pela Universidade Estadual de Maringá, o mesmo jamais será submetido por mim ou por qualquer um dos demais co-autores a qualquer outro meio de divulgação científica. Através deste instrumento, em meu nome e em nome dos demais co-autores, porventura existentes, cedo os direitos autorais do referido artigo à Universidade Estadual de Maringá e declaro estar ciente de que a não observância deste compromisso submeterá o infrator a sanções e penas previstas na Lei de Proteção de Direitos Autorias (Nº9609, de 19/02/98).
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