| Registro completo |
| Provedor de dados: |
101
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| País: |
Cuba
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| Título: |
Influence of culture medium components on Mycoplasma gallisepticum growth
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| Autores: |
Pérez Bueno,Tania
Sánchez Miranda,Lilian
Rosado Ruiz-Apodaca,Ileana
Díaz Bravo,Tito
Rodríguez Edreira,Mercedes
Martínez Marrero,Siomara
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| Data: |
2014-08-01
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| Ano: |
2014
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| Palavras-chave: |
Culture medium optimization
Mycoplasma gallisepticum
Statistical experimental design
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| Resumo: |
The composition of a culture medium produced in Cuba to grow Mycoplasma gallisepticum was evaluated using a response surface methodology. Initially, the influence of Mycoplasma base, horse serum and yeast extract on biomass production was assessed in a 2³ factorial experimental design. Then, a central composite design was applied. Mycoplasma base and horse serum factors were selected for this study. In 7 L and 35 L bioreactors, the variations carried out in the culture medium composition were estimated. The experimental results were fitted to a second order polynomial model equation. Finally, the optimal growth conditions established were at concentrations of 1,88% Mycoplasma base and 18,16% horse serum (v/v) with 0,6% of yeast extract. For the conditions tested, at a bioreactors scale, a high cellular yield of M. gallisepticum (higher than 0,5 ODu/mL) was obtained.
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| Tipo: |
Journal article
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| Idioma: |
Inglês
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| Identificador: |
http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0253-570X2014000200009
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| Editor: |
Centro Nacional de Sanidad Agropecuaria
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| Formato: |
text/html
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| Fonte: |
Revista de Salud Animal v.36 n.2 2014
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