| Registro completo |
| Provedor de dados: |
Zoologia
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| País: |
Brazil
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| Título: |
Evaluation of lugol solution used for counting rumen ciliates
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| Autores: |
D'Agosto,Marta
Carneiro,Maria Elisa
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| Data: |
1999-01-01
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| Ano: |
1999
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| Palavras-chave: |
Rumen ciliate
Quantitative evaluation
Lugol solution
Technique
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| Resumo: |
The present study proposes a modification of the technique described by PURSER & MOIR (1959) for the quantitative evaluation of rumen ciliate based on an adaptation described by DEHORITY (I984). The modifying process includes: the replacement of two drops of brilliant green dye, for at least four hours, by three drops of lugol solution, for at least 15 minutes. It was made a comparative evaluation of these stainings. It was concluded that lugol solution can replace the brilliant green dye showing the following advantages: staining time reduction and subsequent speeding of sample processing; evidence of skeletal plates of entodiniomorphs making its identification easier; improved observation of small ciliates and inconspicuous structures; improved total counting and generic identification of the ciliates.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-81751999000300011
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| Editor: |
Sociedade Brasileira de Zoologia
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| Relação: |
10.1590/S0101-81751999000300011
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| Formato: |
text/html
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| Fonte: |
Revista Brasileira de Zoologia v.16 n.3 1999
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| Direitos: |
info:eu-repo/semantics/openAccess
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