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Provedor de dados:  Agrociencia
País:  Mexico
Título:  Pollen grain viability in accessions of Crotalaria juncea L. (FABACEAE)
Autores:  Coelho,Ana P. D.
Morais,Katiule P.
Dail Laughinghouse IV,Haywood
Giacomini,Sandro J.
Tedesco,Solange B.
Data:  2012-08-01
Ano:  2012
Palavras-chave:  Crotalaria juncea Alexander's stain
Acetic orcein
Pollen viability
Plant cytogenetics
Resumo:  Studies on pollen viability are useful for breeding Crotalaria juncea L. since they provide information that will increase the chance of successful intraspecific crossing. The aim of this study was to evaluate pollen viability in C. juncea accessions cultivated in Rio Grande do Sul, Brazil. Inflorescences of 10 accessions of C. juncea were collected, fixed in absolute ethanol: glacial acetic acid (3:1) for 72 h at room temperature and placed in 70 % ethanol. Analysis of pollen viability was performed with slides of anthers using the squashing technique and staining with acetic orcein 2 % and Alexanders stain. Means were compared using the Tukey test (p≤0.05). Pollen viability was high and significantly different among the accessions; the highest value was 99.67 % and the lowest 45.34 %. However, unviable pollen grains were also observed in all accessions.
Tipo:  Info:eu-repo/semantics/article
Idioma:  Inglês
Identificador:  http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S1405-31952012000500005
Editor:  Colegio de Postgraduados
Formato:  text/html
Fonte:  Agrociencia v.46 n.5 2012
Direitos:  info:eu-repo/semantics/openAccess
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