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Provedor de dados:  Repositório Alice
País:  Brazil
Título:  Identification of a seed maturation protein gene from Coffea arabica (CaSMP) and analysis of its promoter activity in tomato.
Autores:  QUINTERO, F. O. C.
PINTO, L. G.
BARSALOBRES-CAVALLARI, C. F.
ARCURI, M. de L. C.
PINO, L. E.
PERES, L. E. P.
MALUF, M. P.
MAIA, I. G.
Data:  2019-10-21
Ano:  2018
Palavras-chave:  Tomato
Promoter
Seed maturation protein
Seed-specific expression
Coffee
Café
Resumo:  In this report, an expressed sequence tag-based approach was used to identify a seed-specific candidate gene for promoter isolation in Coffea arabica. The tissue-specific expression of the cognate gene (CaSMP), which encodes a yet uncharacterized coffee seed maturation protein, was validated by RT-qPCR. Additional expression analysis during coffee fruit development revealed higher levels of CaSMP transcript accumulation in the yellow/green phenological stage. Moreover, CaSMP was preferentially expressed in the endosperm and was down-regulated during water imbibition of the seeds. The presence of regulatory cis-elements known to be involved in seed- and endosperm-specific expression was observed in the CaSMP 5?-upstream region amplified by genome walking (GW). Additional histochemical analysis of transgenic tomato (cv. Micro-Tom) lines harboring the GW-amplified fragment (~ 1.4 kb) fused to uidA reporter gene confirmed promoter activity in the ovule of immature tomato fruits, while no activity was observed in the seeds of ripening fruits and in the other organs/tissues examined. These results indicate that the CaSMP promoter can be used to drive transgene expression in coffee beans and tomato seeds, thus representing a promising biotechnological tool.

bitstream/item/203217/1/Identificaction-of-a-seed-maturation-protein.pdf
Tipo:  Artigo em periódico indexado (ALICE)
Idioma:  Inglês
Identificador:  1324

http://www.alice.cnptia.embrapa.br/alice/handle/doc/1113247
Editor:  Plant Cell Reports, v. 37, n.9, p.1257–1268, September 2018.
Relação:  Embrapa Café - Artigo em periódico indexado (ALICE)
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