| Registro completo |
| Provedor de dados: |
Nature Precedings
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| País: |
United Kingdom
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| Título: |
Genome sequence and genetic linkage analysis of Shiitake mushroom _Lentinula edodes_
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| Autores: |
Hoi Shan Kwan
Chun Hang Au
Man Chun Wong
Jing Qin
Iris Sze Wai Kwok
Winnie Wing Yan Chum
Pui Ying Yip
Kin Sing Wong
Lei Li
Qian Li Huang
Wen Yan Nong
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| Data: |
2012-01-31
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| Ano: |
2012
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| Palavras-chave: |
Genetics & Genomics
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| Resumo: |
_Lentinula edodes_ (Shiitake/Xianggu) is an important cultivated mushroom. Understanding the genomics and functional genomics of _L. edodes_ allows us to improve its cultivation and quality. Genome sequence is a key to develop molecular genetic markers for breeding and genetic manipulation. We sequenced the genome of _L. edodes_ monokaryon L54A using Roche 454 and ABI SOLiD genome sequencing. Sequencing reads of about 1400Mb were de novo assembled into a 40.2 Mb genome sequence. We compiled the genome sequence into a searchable database with which we have been annotating the genes and analyzing the metabolic pathways. In addition, we have been using many molecular techniques to analyze genes differentially expressed during development. Gene ortholog groups of _L. edodes_ genome sequence compared across genomes of several fungi including mushrooms identified gene families unique to mushroom-forming fungi. We used a mapping population of haploid basidiospores of dikaryon L54 for genetic linkage analysis. High-quality variations such as single nucleotide polymorphisms, insertions, and deletions of the mapping population formed a high-density genetic linkage map. We compared the linkage map to the _L. edodes_ L54A genome sequence and located selected quantitative trait loci. The Shiitake community will benefit from these resources for genetic studies and breeding.

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| Tipo: |
Poster
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| Identificador: |
http://precedings.nature.com/documents/6855/version/1
oai:nature.com:10.1038/npre.2012.6855.1
http://dx.doi.org/10.1038/npre.2012.6855.1
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| Fonte: |
Nature Precedings
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| Direitos: |
Creative Commons Attribution 3.0 License
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