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Provedor de dados:  Nature Precedings
País:  United Kingdom
Título:  Modelling Hepatic Endoderm Development: Highly Efficient Differentiation of Human Embryonic Stem Cells to Functional Hepatic Endoderm Requires ActivinA and Wnt3a Signalling.
Autores:  David Hay
Judy Fletcher
Catherine Payne
John D. Terrace
Ronald C. J. Gallagher
Jan Snoeys
Jim Black
Davina Wojtacha
Kay Samuel
Zara Hannoun
Anne Pryde
Celine Filippi
Ian S. Currie
Stuart J. Forbes
James A. Ross
Philip Newsome
John Iredale
Data:  2007-12-22
Ano:  2007
Palavras-chave:  Biotechnology
Developmental Biology
Resumo:  Human embryonic stem cells (hESCs) are a valuable source of pluripotential primary cells. However, their homogeneous cellular differentiation to specific cell types _in vitro_ has proven difficult thus far. Wnt signalling has been shown to play important roles in coordinating development and we demonstrate that Wnt3a is differentially expressed at critical stages of human liver development _in vivo_. The essential role of Wnt3a in hepatocyte differentiation from hESCs is paralleled by our _in vitro_ model, demonstrating the importance of a physiological approach to cellular differentiation. Our studies provide compelling evidence that Wnt3a signaling is important for coordinated hepato-cellular function _in vitro_ and _in vivo_. In addition, we demonstrate Wnt3a facilitates clonal plating of hESCs capable of hepatic endoderm differentiation. These studies represent an important step forward toward the use of hESC-derived hepatocytes in biomedical applications and has opened the door to high through-put metabolic analysis of human liver function.
Tipo:  Manuscript
Identificador:  http://precedings.nature.com/documents/1454/version/1

oai:nature.com:10101/npre.2007.1454.1

http://hdl.handle.net/10101/npre.2007.1454.1
Fonte:  Nature Precedings
Direitos:  Creative Commons Attribution 3.0 License
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