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Provedor de dados:  Inra
País:  France
Título:  Secretome analysis of Phanerochaete chrysosporium strains CIRM-BRFM41 grown on softwood
Autores:  Ravalason, H.
Jan, G.
Mollé, D.
Pasco, M.
Coutinho, P.M.
Lapierre, C.
Pollet, B.
Bertaud, F.
Petit-Conil, M.
Grisel, S.
Sigoillot, J.C.
Asther, M.
Gimbert, I.
Data:  2008
Ano:  2008
Phanerochaete chrysosporium
Resumo:  Abstract Proteomic analysis was performed to determine and differentiate the composition of the secretomes of Phanerochaete chrysosporium CIRM-BRFM41, a peroxidasehypersecretory strain grown under ligninolytic conditions and on softwood chips under biopulping conditions.Extracellular proteins from both cultures were analyzed by bidimensional gel electrophoresis and matrix-assisted laserdesorption/ionization time-of-flight tandem mass spectrometry.A total of 37 spots were identified. The secretome in liquid synthetic medium comprised mainly peroxidases,while several wood-degrading enzymes and enzymes involved in fungal metabolism were detected in biopulping cultures on softwood. This prompted an analysis of the impact of secretome modulation in the presence ofsoftwood chips. Biotreated wood was submitted to kraft cooking and chemical bleaching using chlorine dioxide.The fungal pre-treatment led to a significant increase in pulp yield and a better bleachability of the pulp. Thisbleachability improvement could be explained by the production of specific lignocellulose-degrading enzymes.
Tipo:  Journal Article
Idioma:  Inglês
Fonte:  Applied Microbiology and Biotechnology. 2008, 80 (4) : 719-733

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