| Registro completo |
| Provedor de dados: |
OAK
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| País: |
Japan
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| Título: |
Ouabain exacerbates botulinum neurotoxin-induced muscle paralysis via progression of muscle atrophy in mice
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| Autores: |
Fujikawa, Ryu
Muroi, Yoshikage
Unno, Toshihiro
Ishii, Toshiaki
室井, 喜景
石井, 利明
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| Data: |
2010
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| Ano: |
2010
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| Palavras-chave: |
Ouabain
Botulinum neurotoxin
Muscle atrophy
MAFbx
MuRF-1
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| Resumo: |
Botulinum neurotoxin serotype A (BoNT/A) inhibits acetylcholine release at the neuromuscular junction in isolated muscles, and ouabain can partially block its effect. However, it is not clear whether ouabain attenuates BoNT/A-induced neuromuscular paralysis in vivo. In this work, we investigated the effects of ouabain on BoNT/A-induced neuromuscular paralysis in mice. Ouabain was administered to mice intraperitoneally immediately after a single injection of BoNT/A into skeletal muscle. The effects of ouabain on BoNT/A-induced muscle paralysis were assessed by quantitative monitoring of muscle tension and digit abduction via the digit abduction scoring (DAS) assay. A single administration of ouabain significantly prolonged BoNT/A-induced neuromuscular paralysis. Moreover, consecutive daily injection of ouabain exacerbated BoNT/A-induced neuromuscular paralysis, and led to a significant decrease in both twitch and tetanic forces as assayed in isolated BoNT/A-injected muscles. We next looked at the effects of ouabain on BoNT/A-induced muscle atrophy. Administration of ouabain led to a decrease in the myofibrillar cross-sectional area (CSAs) by 14 post-BoNT/A injection. In addition, repeated administration of ouabain increased mRNA expression levels of ubiquitin ligases, which are markers of muscle atrophy, in BoNT/A-injected muscle. These results suggest that ouabain exacerbates BoNT/A-induced neuromuscular paralysis via a marked progression of BoNT/A-induced muscle atrophy.
http://www.jstage.jst.go.jp/article/jts/35/6/35_795/_article/-char/ja/
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| Idioma: |
Inglês
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| Identificador: |
http://ir.obihiro.ac.jp/dspace/handle/10322/3123
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| Editor: |
日本毒性学会
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| Formato: |
application/pdf
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| Direitos: |
日本毒性学会
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