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Provedor de dados:  OAK
País:  Japan
Título:  Cloning and Characterization of a 2-Cys Peroxiredoxin from Babesia gibsoni
Autores:  MASATANI, Tatsunori
ASADA, Masahito
ICHIKAWA-SEKI, Madoka
USUI, Miho
TERKAWI, Mohamad A.
HAYASHI, Kei
KAWAZU, Shin-ichiro
XUAN, Xuenan
河津, 信一郎
玄, 学南
Data:  2014
Ano:  2014
Palavras-chave:  Antioxidant activity
Babesia gibsoni
Canine
Peroxiredoxin
Resumo:  Peroxiredoxins (Prxs) are a family of antioxidant enzymes. Here, we cloned a 2-Cys Prx, BgTPx-1, from the canine Babesia parasite B. gibsoni. Sequence identity between BgTPx-1 and 2-Cys Prx of B. bovis was 81% at the amino acid level. Enzyme activity assay by using recombinant BgTPx-1 (rBgTPx-1) indicated that BgTPx-1 has antioxidant activity. Antiserum from a mouse immunized with rBgTPx-1 reacted with parasite lysates and detect a protein with a monomeric size of 22 kDa and also a 44 kDa protein, which might be an inefficiently reduced dimer. BgTPx-1 was expressed in the cytoplasm of B. gibsoni merozoites. These results suggest that the BgTPx-1 may play a role to control redox balance in the cytoplasm of B. gibsoni.

https://www.jstage.jst.go.jp/article/jvms/76/1/76_13-0274/_article/-char/ja/
Idioma:  Inglês
Identificador:  http://ir.obihiro.ac.jp/dspace/handle/10322/3925
Editor:  日本獣医学会
Direitos:  日本獣医学会
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