| Registro completo |
| Provedor de dados: |
OAK
|
| País: |
Japan
|
| Título: |
トキソブラズマ溶解抗原(TLA)によって誘導される細胞障害性における脾臓の付着性細胞の役割
Role of Adherent Spleen Cells in the Induction of Cytotoxic Activity by Toxoplasma Lysate Antigen
|
| Autores: |
MIYAHARA, Kazuro
TOSE, Shinichi
SAKURAI, Haruhisa
IGARASHI, Ikuo
SAITO, Atsushi
HIROSE, Tsuneo
SUZUKI, Naoyoshi
宮原, 和郎
五十嵐, 郁男
|
| Data: |
1992
|
| Ano: |
1992
|
| Palavras-chave: |
Adherent cell
Cytotoxic activity
Macrophage
Spleen cell
Toxoplasma lysate antigen
|
| Resumo: |
In order to identify mechanisms responsible for the anti-tumor effects of Toxoplasma lysate antigen (TLA), we used an in vitro 51Cr release assay to study the functional properties of plastic-adherent cells during induction of splenic cytotoxic activity by TLA. Cytotoxic activity of non-adherent cells was measured in all experiments after a 6 days incubation. Induction of cytotoxic non-adherent cells by TLA required the presence of plastic-adherent spleen cells. In contrast, rhIL-2 alone was able to induce transformation of cytotoxic non-adherent cells from non-adherent spleen cells. Contact between adherent and non-adherent spleen cells was necessary for successful induction of cytotoxic non-adherent cells by TLA. Treatment of spleen cells with anti-macrophage serum prevented induction of cytotoxic activity by TLA. Biologically active IL-2 was not detected in culture supernatants of spleen cells exposed to TLA. These findings suggest that contact between TLA-sensitized non-adherent cells and macrophages is necessary for induction of cytotoxic cells in the presence of TLA. This contact, however, is not necessary for generation of IL-2-induced killer cells.
https://www.jstage.jst.go.jp/article/jvms1991/54/4/54_4_629/_article
|
| Idioma: |
Inglês
|
| Identificador: |
http://ir.obihiro.ac.jp/dspace/handle/10322/4065
|
| Editor: |
日本獣医学会
|
| Direitos: |
日本獣医学会
|
|
