| Registro completo |
| Provedor de dados: |
OAK
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| País: |
Japan
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| Título: |
アルファルファとVerticillium albo-atrumの相互作用 : 6. 菌体細胞壁成分に対して高いPAL活性を示す細胞系統の選抜とその特性
Alfalfa-Verticillium albo-atrum Interactions : 6. Selection and characterization of high PAL cell lines in response to fungal cell wall components
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| Autores: |
Koike, Masanori
Katsumata, Yukiyoshi
Amemiya, Yoshimiki
Shimada, Tohru
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| Data: |
1995-07-30
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| Ano: |
1995
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| Palavras-chave: |
Alfalfa
In vitro selection
Phenylalanine ammonia-lyase
アルファルファ
細胞選抜
Verticillium albo-atrum
フェニルアラニンアンモニアリアーゼ
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| Resumo: |
To obtain alfalfa cell lines with high phenylalanine ammonia-lyase (PAL) activity in response to heat-released cell wall components (elicitor) of an alfalfa pathogen (Verticillium albo-atrum), in vitro selection was performed. The selection using a replica plating methods was carried out on 65 cell lines derived from suspension cultures treated and/or non-treated with N-metyl-N'-nitro-N-nitrosoguanidine (MNNG) of the susceptible genotype of cv. Vertus. Three stable high PAL cell lines in response to elicitor (two cell lines recovered from MNNG treatment, one from non-MNNG treatment) were isolated. All these cell lines showed higher PAL activities, lignin depositions and phytoalexin accumulations than the parental cell line after treatment with active V. albo-atrum conidia. These results indicate that PAL may be a good marker for in vitro selection for Alfalfa Verticillium wilt.
日本草地学会Journal of Japanese Society of Grassland Science 41(2):128-132(1995)より転載
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| Idioma: |
Inglês
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| Identificador: |
http://ir.obihiro.ac.jp/dspace/handle/10322/1770
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| Editor: |
Japanese Society of Grassland Science
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| Formato: |
application/pdf
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