| Registro completo |
| Provedor de dados: |
OAK
|
| País: |
Japan
|
| Título: |
Identification and characterization of cross-reactive antigens from Neospora caninum and Toxoplasma gondii
|
| Autores: |
Liao, Min
Xuan, Xuenan
Huang, Xiaohong
Shirafuji, Hiroaki
Fukumoto, Shinya
Hirata, Haruyuki
Suzuki, Hiroshi
Fujisaki, Kozo
|
| Data: |
2005-05
|
| Ano: |
2005
|
| Palavras-chave: |
Neospora caninum
Toxoplasma gondii
Cross-reactive antigen
Protein disulfide isomerase
Heat-shock protein
Ribosomal protein 1
|
| Resumo: |
Murine monoclonal antibodies (mAbs) against Neospora caninum tachyzoites were produced to identify the cross-reactive antigens between N. caninum and Toxoplasma gondii. Ten mAbs recognizing cross-reactive antigens of both parasites were obtained and tentatively classified into 6 different groups based on their reactivity patterns in an indirect fluorescent antibody test and Western blot analysis. Three mAbs in group I recognized antigens located on the surface of parasites with molecular masses ranging from 28 to 76 kDa; one mAb in group 2 recognized antigens located on interior organelles of parasites with a molecular mass of 50 kDa; one mAb in group 3 recognized antigens located on interior organelles of parasites with molecular masses of 35 kDa and 14 kDa; three mAbs in group 4 recognized antigens located on interior organelles with a molecular mass of 64 kDa; one mAb in group 5 recognized antigens located on the surface of parasites with an unknown molecular mass; one mAb in group 6 recognized antigens located on the apical end of parasites with an unknown molecular mass. The mAbs in groups 1, 2, 3, and 5 showed inhibitory effects on the growth of the two parasites in vitro in a dose-dependent manner. A cDNA expression library prepared from N. caninum tachyzoite mRNA was immunoscreened with the mAb panel. Three kinds of proteins, protein disulfide isomerase (PDI), heat-shock protein 70 (HSP70), and ribosomal protein 1 (RP1), were identified as cross-reactive antigens recognized by mAbs in groups 2, 3, and 4, respectively. Some of the proteins could be useful in developing vaccines or drugs for controlling the diseases caused by the two parasites.
|
| Idioma: |
Inglês
|
| Identificador: |
http://ir.obihiro.ac.jp/dspace/handle/10322/816
|
| Editor: |
CAMBRIDGE UNIV PRESS
|
| Formato: |
application/pdf
|
|
