| Registro completo |
| Provedor de dados: |
Animal Sciences
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| País: |
Brazil
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| Título: |
Commercial and non-commercial pectinase and cellulase on the enzymatic hydrolysis efficacy of rice husk and Tifton 85 hay
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| Autores: |
Teixeira, Amito José
Weschenfelder, Leonardo Menoncin
Antunes, Angela
Zeni, Jamile
Backes, Geciane Toniazzo
Cansian, Rogério Luis
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| Data: |
2019-04-08
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| Ano: |
2019
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| Palavras-chave: |
5.00.00.00-4 Enzyme
Fiber
Forage
Residues
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| Resumo: |
The aim of this study was to evaluate the action of commercial and non-commercial cellulase and pectinase on rice husk and Tifton 85 hay hydrolyses. The hydrolysis kinetics of the substrates with commercial cellulase and pectinase were evaluated and the hydrolysis at different temperature and agitation conditions was maximized using experimental design. The combined use of commercial and non-commercial enzymes under optimized conditions was evaluated. The pre-treatment of the residues was also investigated by milling and different concentrations of NaOH. Finally, the effect of the hydrolysis on the bromatological composition of the residues was evaluated. The best hydrolysis times of rice husk and Tifton 85 hay were 10 and 12h for commercial cellulase, 12 and 14h for non-commercial cellulase, 10 and 14h for commercial pectinase and 16 and 20h for non-commercial pectinase, respectively. The highest hydrolysis values were obtained using commercial cellulase with 1:50 (w:v enzyme:water) dilution rate, at 45ºC and 300 rpm agitation for both substrates, reaching 20.6% maximum percentage for Tifton 85 hay and 11.6% for rice husk. The combined use of commercial enzymes did not increase hydrolysis percentage. The pre-treatment using 7.5% NaOH and 0.5 mm grain size significantly increased the rice husk and Tifton 85 hay hydrolyses (60-80%), either using commercial cellulase or pectinase enzymes. The use of non-commercial enzymes provided 18-30% hydrolysis obtained from commercial ones. Bromatological analyzes indicated a reduction in neutral detergent fiber and acid detergent fiber content for rice husk and Tifton 85 hay when using pectinases and commercial cellulases.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/45100
10.4025/actascianimsci.v41i1.45100
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| Editor: |
Editora da Universidade Estadual de Maringá
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| Relação: |
http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/45100/pdf
http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/downloadSuppFile/45100/20785
http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/downloadSuppFile/45100/20786
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| Formato: |
application/pdf
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| Fonte: |
Acta Scientiarum. Animal Sciences; v. 41 (2019): Publicação Contínua; e45100
Acta Scientiarum. Animal Sciences; v. 41 (2019): Publicação Contínua; e45100
1807-8672
1806-2636
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| Direitos: |
Direitos autorais 2019 Acta Scientiarum. Animal Sciences
http://creativecommons.org/licenses/by/4.0
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