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| Provedor de dados: |
OceanDocs
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| País: |
Belgium
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| Título: |
Aislamiento e identificación de bacterias presentes en cultivos de microalgas marinas. Actividad antibacteriana de algunas de las especies encontradas
Isolation and identification of bacteria present in marine microalgae cultures. Antibacterial activity of some of the species found
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| Autores: |
Hernández-Zulueta, J.
Leal, S.
Lugioyo, G.M.
Loza, S.
Curbelo, R.
Caballero, V.M.
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| Data: |
2013-03-12
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| Ano: |
2011
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| Palavras-chave: |
Check lists
Isolation (genetics)
Algae culture
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| Resumo: |
El presente estudio de las siguientes especies de microalgas marinas tuvo como objetivo identificar y determinar la actividad antibacteriana de la microbiota presente en los cultivos de las siguientes especies de microalgas marinas: Chaetoceros ceratosporum, Chaetoceros muelleri, Thalassiosira sp., Thalassiosira weisflogii, Amphora sp., Navicula sp. y Tetraselmis suecica, especies que se utilizan en la alimentación de animales acuáticos. Se estudiaron las características morfológicas, culturales y tintoriales de las cepas aisladas, así como las características fisiológicas y bioquímicas. Para la clasificación se utilizó el Manual de Bergey (Krieg & Holt, 1984; Sneath et al., 1986). La actividad antibacteriana se determinó mediante el método de difusión en placa donde se empleó el medio de cultivo agar nutriente y bloques de agar con los cultivos a evaluar. Se aislaron e identificaron 70 bacterias, de las cuales el 25,7 % fueron productoras de sustancias antibacterianas. Las cepas con mayores actividades antibacterianas fueron Bacillus cereus (en cultivos de Thalassiosira sp. y Tetraselmis suecica), y B. megaterium (en cultivos de Chaetoceros ceratosporum) que inhibieron a cuatro de las cepas patógenas ensayadas. Los resultados sugieren que las bacterias aisladas podrían ser aprovechadas como agentes probióticos en el control biológico de patógenos de organismos marinos de interés en maricultivo.
In this paper, we report the identification and actibacterial activity of marine bacterial strains associated in the marine microalgal cultures Chaetoceros ceratosporum, Chaetoceros muelleri, Thalassiosira sp., Thalassiosira weisflogii, Amphora sp., Navicula sp. and Tetraselmis suecica. The antibacterial activity was determined by the diffusion plate method using Nutrient agar and agar blocks with the cultures to study. As result, 70 marine bacterial strains were isolated and identified, the 25,7 % of the bacterial microbiota was able to produce antibacterial substances. The strains with most antibacterial activity were Bacillus cereus (Thalassiosira sp.), B. cereus (Tetraselmis suecica), and B. megaterium (Chaetoceros ceratosporum) that inhibited four of the pathogens strains assayed. The results suggest that the isolated bacteria could be used as probiotics agents for the biological control of pathogens from marine organisms of interest in mariculture.
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| Tipo: |
Journal Contribution
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| Idioma: |
Espanhol
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| Identificador: |
Revista cubana de investigaciones pesqueras, 28 (2), p. 59-67
0138-8452
http://hdl.handle.net/1834/4651
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| Relação: |
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