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Provedor de dados:  OMA
País:  Belgium
Título:  Effect of different light regimes on the docosahexaenoic acid (DHA) content of <i>Isochrysis</i> aff. <i>galbana</i> (clone T-ISO)
Autores:  Tzovenis, I.
De Pauw, N.
Sorgeloos, P.
Data:  1997
Ano:  1997
Palavras-chave:  Rhodomonas salina
Resumo:  The culture of <i>Isochrysis</i> aff. <i>galbana</i> Green (T-ISO) in hatcheries is promoted for its high docosahexaenoic acid (DHA) content which favours the successful rearing of aquatic larvae. In this paper we identified the need for well-defined culture conditions of microalgae if sufficient DHA content is the final goal. Using photoperiods of 24:0h, 12:12h and 16:8h L/D combined with photon flux densities (PFD's) of 110, 220 and 460 µE m<sup>-2</sup> s<sup>-1</sup>, batch cultures of T-ISO were sampled for DHA content in the exponential as well as the post-exponential (light-limited) growth phase. The various light regimes resulted in differences of DHA content of T-ISO revealing influences and strong interactions among the three variables studied. For fast and economic production of T-ISO cells rich in DHA it is recommended to harvest batch cultures at the end of the exponential phase using high photon flux densities (PFD's) under 12:12 h L/D cycles. The same regime or lower PFD under 24:0 h L/D seem to be sufficient for a continuous culture optimization scheme. In any case a strict following of culture protocols is very important since there is evidence for coexistence of different clones within the parental cultures leading to different results depending on which one prevails.
Tipo:  Info:eu-repo/semantics/article
Idioma:  Inglês
Identificador:  http://www.vliz.be/nl/open-marien-archief?module=ref&refid=2979
Fonte:  %3Ci%3EAquacult.+Int.+5%3C%2Fi%3E%3A+489-507
Direitos:  info:eu-repo/semantics/restrictedAccess
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