| Registro completo |
| Provedor de dados: |
OMA
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| País: |
Belgium
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| Título: |
Application of AFLP for taxonomic and epidemiological studies of <i>Photobacterium damselae</i> subsp. <i>piscicida</i>
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| Autores: |
Thyssen, A.
Van Eygen, S.
Hauben, L.
Goris, J.
Swings, J.
Ollevier, F.P.
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| Data: |
2000
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| Ano: |
2000
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| Palavras-chave: |
Epidemiology
Taxonomy
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| Resumo: |
A collection of 106 <i>Photobacterium damselae</i> subsp. <i>piscicida</i> strains and 19 <i>Photobacterium damselae</i> subsp. <i>damselae</i> strains, including reference and type strains, were genetically characterized using AFLP. The total genomic DNA of each bacterial strain was digested using restriction endonucleases <i>Hind</i>III and <i>Taq</i>I. Using numerical analysis, six clusters were recognized. The largest cluster (n=106) contained the majority of the strains tested and consisted exclusively of <i>Photobacterium damselae</i> subsp. <i>piscicida</i>. The <i>Photobacterium damselae</i> subsp. <i>damselae</i> strains fell outside this cluster. DNA-DNA hybridization experiments showed 77% DNA binding between the two subspecies, indicating a close genetic relationship. This clearly demonstrates the applicability of AFLP in studying the taxonomic position of <i>Photobacterium damselae</i> subsp. <i>piscicida</i>. In addition, AFLP proved to be a useful genotypic technique for epidemiological surveys of the pathogen, since it was able to discriminate between Mediterranean and Japanese <i>Photobacterium damselae</i> subsp. <i>piscicida</i> isolates.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.vliz.be/nl/open-marien-archief?module=ref&refid=9527
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| Fonte: |
%3Ci%3EInt.+J.+Syst.+Bacteriol.+50%3C%2Fi%3E%3A+1013-1019
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| Direitos: |
info:eu-repo/semantics/restrictedAccess
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