| Registro completo |
| Provedor de dados: |
OMA
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| País: |
Belgium
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| Título: |
The nuclear rDNA region of <i>Gyrodactylus arcuatus</i> and <i>G. branchicus</i> (Monogenea: Gyrodactylidae)
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| Autores: |
Zietara, M.S.
Arndt, A.
Geets, A.
Hellemans, B.
Volckaert, F.A.M.J.
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| Data: |
2000
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| Ano: |
2000
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| Palavras-chave: |
DNA
Molecular structure
Nucleotide sequence
Taxonomy
Gasterosteus aculeatus
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| Resumo: |
The primary structure of the ribosomal DNA internal transcribed spacers (ITS-1 and ITS-2) and 5.8S rRNA gene were used to characterize and identify 2 monogenean species of <i>Gyrodactylus</i> living externally on the threespine stickleback (<i>Gasterosteus aculeatus</i>). The ITS region was amplified by PCR from freshwater, brackish, and marine isolates of <i>Gyrodactylus arcuatus</i> and <i>G. branchicus</i>, and the ends of the coding regions were identified by comparative alignment. No intraspecific and very low interspecific variation were observed in the 5.8S rRNA gene; high inter- and low intraspecific variation were revealed in the ITS-1 and ITS-2 regions. The morphological species identification was in all cases confirmed by the molecular identification. Intraspecifically, samples from 2 locations in the North Sea could be differentiated, but the Baltic sample resembled North Sea genotypes. Our approach offers perspectives for a multimetric genetical, morphometrical, and ecological taxonomy of the genus <i>Gyrodactylus</i>.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.vliz.be/nl/open-marien-archief?module=ref&refid=8482
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| Relação: |
info:eu-repo/semantics/altIdentifier/wos/000166184200035
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| Fonte: |
%3Ci%3EJ.+Parasitol.+86%286%29%3C%2Fi%3E%3A+1368-1373.+%3Ca+href%3D%22https%3A%2F%2Fhdl.handle.net%2F10.2307%2F3285031%22+target%3D%22_blank%22%3Ehttps%3A%2F%2Fhdl.handle.net%2F10.2307%2F3285031%3C%2Fa%3E
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| Direitos: |
info:eu-repo/semantics/restrictedAccess
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