| Registro completo |
| Provedor de dados: |
OMA
|
| País: |
Belgium
|
| Título: |
Liquid chromatographic determination of efficacy of incorporation of trimethoprim and sulfamethoxazole in brine shrimp (<i>Artemia</i> spp.) used for prophylactic chemotherapy of fish
|
| Autores: |
Nelis, H.
Léger, P.
Sorgeloos, P.
De Leenheer, A.P.
|
| Data: |
1991
|
| Ano: |
1991
|
| Palavras-chave: |
Aquaculture techniques
Fish culture
Infectious diseases
Medicine
Artemia
|
| Resumo: |
The brine shrimp <i>Artemia</i> , an excellent live food source in aquaculture, has been studied as a carrier to deliver selected chemotherapeutic agents to fish for prophylactic treatment of infectious diseases. To monitor the efficiency of incorporation of trimethoprim and sulfamethoxazole in <i><i>Artemia</i> franciscana</i>, a sensitive and specific analytical method was developed. It is based on homogenization of <i>Artemia</i> nauplii in methanol, extraction of lipids with hexane, solid-phase cleanup on C<sub>18</sub> cartridges, and reversed-phase liquid chromatography with detection at 210 nm. The method is sensitive (detection limit, on the order of 3 µg/g with a sample quantity of 30 mg [dry weight]) and reproducible (coefficients of variation, 2.2 and 1.8% for trimethoprim and sulfamethoxazole at levels of 79.6 and 257 µg/g of body weight, respectively). Preliminary quantitative data indicated excellent uptake and persistence of both therapeutic agents in <i>A. franciscana</i>, with levels of 115 µg/g for trimethoprim and 277 µg/g for sulfamethoxazole.
|
| Tipo: |
Info:eu-repo/semantics/article
|
| Idioma: |
Inglês
|
| Identificador: |
http://www.vliz.be/imisdocs/publications/262329.pdf
|
| Formato: |
application/pdf
|
| Fonte: |
%3Ci%3EAntimicrob.+Agents+Chemother.+35%2812%29%3C%2Fi%3E%3A+2486-2489
|
| Direitos: |
info:eu-repo/semantics/openAccess
|
|
