| Registro completo |
| Provedor de dados: |
Biological Sciences
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| País: |
Brazil
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| Título: |
Isolation of Saprolegnia aenigmatica oomycetes and protocol for experimental infection of pacu (Piaractus mesopotamicus)
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| Autores: |
Carraschi, Silvia Patrícia
Garlich, Nathália
Souza-Pollo, Andressa
Pereira, Daniela Isabel Brayer
Cruz, Claudinei da
Ranzani-Paiva, Maria José Tavares
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| Data: |
2018-04-01
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| Ano: |
2018
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| Palavras-chave: |
5.06.03.00-0 oomycete
Zoosporangium
Oogonium
Saprolegniasis. Aquicultura
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| Resumo: |
Successful disease treatment depends on molecular studies under indoor conditions with experimental infection protocols that facilitate understanding the disease and the drug`s efficacy. The internal transcribed spacer (ITS) region was sequenced from three isolates, which were identified as Saprolegnia aenigmatica. Subsequently, healthy fish were immunosuppressed with dexamethasone (1.2 mg kg-1) and descaled to the skin using a sharp scalpel. These individuals were isolated in individual aquariums maintained at 22°C. Individuals in one group were subcutaneously inoculated with 9,000 zoospores (DDZ treatment), a second group was exposed to oomycetes in water with three colonized baits (DDB), a third group was maintained in water without zoospores (DD), and a control group (C) consisted of healthy animals. After 48 and 96 hours, two animals from each group were euthanized for fungal re-isolation. The fish from groups DD and C did not show clinical signs, and no oomycetes were isolated. The animals from the DDZ and DDB groups showed cotton-wool-like masses on the skin, and S. aenigmatica was re-isolated. Thus, for infection using zoospores or baits parasitized by S. aenigmatica, an immunosuppressor (dexamethasone) and a sharp scalpel can be used effectively to establish an experimental infection in P. mesopotamicus.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/38186
10.4025/actascibiolsci.v40i1.38186
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| Editor: |
Universidade Estadual De Maringá
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| Relação: |
http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/38186/pdf
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| Formato: |
application/pdf
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| Fonte: |
Acta Scientiarum. Biological Sciences; v. 40 (2018): Publicação Contínua; e38186
Acta Scientiarum. Biological Sciences; v. 40 (2018): Publicação Contínua; e38186
1807-863X
1679-9283
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| Direitos: |
Direitos autorais 2018 Acta Scientiarum. Biological Sciences
http://creativecommons.org/licenses/by/4.0
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