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| Provedor de dados: |
Biological Sciences
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| País: |
Brazil
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| Título: |
Establishment and content of sugars and phenols in Physalis callus obtained from different explants and concentrations of bap and naa - doi: 10.4025/actascibiolsci.v36i1.18074
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| Autores: |
Bertoncelli, Douglas Junior; Universidade Tecnológica Federal do Paraná
Oliveira, Marisa de Cacia; Universidade Tecnológica Federal do Paraná
Passos, Amanda Izabel dos; Universidade Tecnológica Federal do Paraná
Ariati, Ana Claudia; Universidade Tecnológica Federal do Paraná
Ortolan, Alexandre Osmar; Universidade Tecnológica Federal do Paraná
Witt, Elaine de Paula; Universidade Tecnológica Federal do Paraná
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| Data: |
2013-09-04
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| Ano: |
2013
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| Palavras-chave: |
2.03.00.00-0 cell
Growth
Phenols
Sugars Botânica-biotecnologia
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| Resumo: |
Obtaining cells of Physalis pubescens is of interest for studies of primary and secondary metabolic pathways, in the search for new active molecules. Our objectives were to evaluate the regeneration potential of explants from different parts of the plant, growth regulators to be used, and the determination of the growth curve of the callus. We used explants of leaf, root, stem and petiole, cultured on Murashige and Skoog medium with different concentrations of 6-benzylaminopurine and a-naphthaleneacetic acid. The explants from stem and petiole had a higher regeneration potential of the shoot to the treatment with 0.5 mg L-1 6-benzylaminopurine, and the explants of leave and root emitted more roots, with lower production of callus. The tests showed that the regeneration of the whole plant should be done in two steps: cultivation for shoot regeneration and transplantation to a new rooting medium. The growth of callus showed five distinct phases, with accumulation of phenols in the final stages of growth. The levels of soluble sugars increased with age, while reducing sugars showed variations, with higher concentrations in the initial stages of cultivation, with fall and rise again at the final evaluation (28th day).
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Português
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| Identificador: |
http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/18074
10.4025/actascibiolsci.v36i1.18074
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| Editor: |
Universidade Estadual De Maringá
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| Relação: |
http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/18074/pdf
http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/18074/pdf_1
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| Formato: |
application/pdf
application/pdf
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| Fonte: |
Acta Scientiarum. Biological Sciences; v. 36, n. 1 (2014); 27-33
Acta Scientiarum. Biological Sciences; v. 36, n. 1 (2014); 27-33
1807-863X
1679-9283
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| Direitos: |
DECLARATION OF ORIGINALITY AND CESSATION OF COPYRIGHTI Declare that current article is original and has not been submitted for publication, in part or in whole, to any other national or international journal. I also declare that once published in the Acta Scientiarum. Biological Sciences, a publication of the Universidade Estadual de Maringá, it will not be submitted by me or by any co-author to any other scientific communication mean. In my name and in the name of co-authors, I shall cede the copyright of the above mentioned article to the Universidade Estadual de Maringá and I declare that I know that the non-observance of this norm may make me liable for the penalties contemplated in the Law for the Protection of Authors’ Rights (Act 9609 of the 19th February 1998).
DECLARAÇÃO DE ORIGINALIDADE E CESSÃO DE DIREITOS AUTORAIS Declaro que o presente artigo é original, não tendo sido submetido à publicação em qualquer outro periódico nacional ou internacional, quer seja em parte ou em sua totalidade. Declaro, ainda, que uma vez publicado na revista Acta Scientiarum. Biological Sciences, editada pela Universidade Estadual de Maringá, o mesmo jamais será submetido por mim ou por qualquer um dos demais co-autores a qualquer outro meio de divulgação científica. Através deste instrumento, em meu nome e em nome dos demais co-autores, porventura existentes, cedo os direitos autorais do referido artigo à Universidade Estadual de Maringá e declaro estar ciente de que a não observância deste compromisso submeterá o infrator a sanções e penas previstas na Lei de Proteção de Direitos Autorias (Nº9609, de 19/02/98).
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