Registro completo |
Provedor de dados: |
Biological Sciences
|
País: |
Brazil
|
Título: |
Evaluation of supplementation of sucrose medium on the synthesis of Zymomonas mobilis bio-products - doi: 10.4025/actascibiolsci.v32i3.1519
|
Autores: |
Borsari, Raquel Renan Jorge; Universidade Estadual de Londrina
Buzato, João Batista; UEL
Silva, Rui Sérgio dos Santos Ferreira da; UEL
Celligoi, Maria Antonia Pedrini Colabone; UEL
|
Data: |
2010-09-15
|
Ano: |
2010
|
Palavras-chave: |
2.08.02.00-5
2.12.02.02-8 Zymomonas mobilis
Fermentation
Biomass
Substrate
Sorbitol
Levan Bioquímica dos microrganismos
Microbiologia industrial e de fermentação Zymomonas mobilis
Fermentation
Biomass
Substrate
Sorbitol
Levan
|
Resumo: |
The effect of the variables pantothenic acid, yeast extract and sodium chloride, as well as the cell permeabilization technique, were investigated on the formation of levan, ethanol, sorbitol and biomass of Zymomonas mobilis, using a 24-1 fraction factorial design. Cell growth was determined by turbidimetry at 605 nm, relating it to a biomass with a dry weight calibration curve. Reducing sugars were quantified according to Somogyi and Nelson. Total sugars were quantified by the phenol-sulfuric acid method, sorbitol by HPLC and ethanol. The levan produced was precipitated by the addition of absolute ethanol and quantified in fructose units. In levan biosynthesis, the variable that had the largest contribution was cell condition. The results suggested that the factors that most affected biomass and ethanol formation were sodium chloride concentration and cell condition that affected negatively on production. For sorbitol, the variable that had a significant effect was permeabilization, which decreased its synthesis. Studies to amplify the range of established factors would be important.
The effect of the variables pantothenic acid, yeast extract and sodium chloride, as well as the cell permeabilization technique, were investigated on the formation of levan, ethanol, sorbitol and biomass of Zymomonas mobilis, using a 24-1 fraction factorial design. Cell growth was determined by turbidimetry at 605 nm, relating it to a biomass with a dry weight calibration curve. Reducing sugars were quantified according to Somogyi and Nelson. Total sugars were quantified by the phenol-sulfuric acid method, sorbitol by HPLC and ethanol. The levan produced was precipitated by the addition of absolute ethanol and quantified in fructose units. In levan biosynthesis, the variable that had the largest contribution was cell condition. The results suggested that the factors that most affected biomass and ethanol formation were sodium chloride concentration and cell condition that affected negatively on production. For sorbitol, the variable that had a significant effect was permeabilization, which decreased its synthesis. Studies to amplify the range of established factors would be important.
|
Idioma: |
Inglês
|
Identificador: |
http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/1519
10.4025/actascibiolsci.v32i3.1519
|
Editor: |
Universidade Estadual De Maringá
|
Formato: |
application/pdf
application/pdf
|
Fonte: |
Acta Scientiarum. Biological Sciences; v. 32, n. 3 (2010); 229-233
Acta Scientiarum. Biological Sciences; v. 32, n. 3 (2010); 229-233
|
Direitos: |
DECLARATION OF ORIGINALITY AND CESSATION OF COPYRIGHTI Declare that current article is original and has not been submitted for publication, in part or in whole, to any other national or international journal. I also declare that once published in the Acta Scientiarum. Biological Sciences, a publication of the Universidade Estadual de Maringá, it will not be submitted by me or by any co-author to any other scientific communication mean. In my name and in the name of co-authors, I shall cede the copyright of the above mentioned article to the Universidade Estadual de Maringá and I declare that I know that the non-observance of this norm may make me liable for the penalties contemplated in the Law for the Protection of Authors’ Rights (Act 9609 of the 19th February 1998).
DECLARAÇÃO DE ORIGINALIDADE E CESSÃO DE DIREITOS AUTORAIS Declaro que o presente artigo é original, não tendo sido submetido à publicação em qualquer outro periódico nacional ou internacional, quer seja em parte ou em sua totalidade. Declaro, ainda, que uma vez publicado na revista Acta Scientiarum. Biological Sciences, editada pela Universidade Estadual de Maringá, o mesmo jamais será submetido por mim ou por qualquer um dos demais co-autores a qualquer outro meio de divulgação científica. Através deste instrumento, em meu nome e em nome dos demais co-autores, porventura existentes, cedo os direitos autorais do referido artigo à Universidade Estadual de Maringá e declaro estar ciente de que a não observância deste compromisso submeterá o infrator a sanções e penas previstas na Lei de Proteção de Direitos Autorias (Nº9609, de 19/02/98).
|
|