| Registro completo |
| Provedor de dados: |
Biological Sciences
|
| País: |
Brazil
|
| Título: |
Molecular characteristics of polysulfated fractions from the green seaweed Caulerpa cupressoides and actions on thrombin generation in vitro
|
| Autores: |
Rodrigues, José Ariévilo Gurgel
Benevides, Norma Maria Barros
Tovar, Ana Maria Freire
Mourão, Paulo Antônio de Souza
|
| Data: |
2019-04-16
|
| Ano: |
2019
|
| Palavras-chave: |
Caulerpaceae
Marine alga
Sulfated glycans
Thrombosis.
|
| Resumo: |
Caulerpa cupressoides produces sulfated polysaccharides (Cc-SPs) with serpin-dependent anticoagulant effect, but their actions on thrombin generation (TG) are unknown. This study aimed to partially characterize Cc-SPs and examine their potential as modulators of TG. Infrared analysis characterized extract containing three ulvan fractions (Cc-SP1, -SP2 and -SP3) separated by DEAE-cellulose chromatography, with differences in the relative proportions of sulfate (10.99-18.38%) and total sugars (46.59-51.12%), without presenting proteins. Charge density patterns and nonSPs varying from 8 to > 100 kDa on agarose and polyacrylamide gel electrophoresis by sequential staining with toluidine blue and stains-all were also confirmed by gel permeation chromatography. The molecular weight of Cc-SP2 was not altered after treatment with 0.4 M HCl up to 5 h. Only Cc-SP2 altered the activated partial thromboplastin time (15 ± 0.3 IU) vs. heparin (193 IU) and abolished at high concentrations (> 4.1 µg) TG by intrinsic pathway in 60-fold diluted human plasma, while at 4.1 µg attenuated TG by 33.87% delaying the lag phase (32 min.) vs. control (28 min.). Cc-SP2 induced concentration-dependent TG in system without cephalin. Heparin abolished TG at 4.15-fold lower amount, but did not stimulate TG. Therefore, Cc-SPs express dual effects on thrombosis in vitro.
|
| Tipo: |
Info:eu-repo/semantics/article
|
| Idioma: |
Inglês
|
| Identificador: |
http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/35655
10.4025/actascibiolsci.v41i1.35655
|
| Editor: |
Universidade Estadual De Maringá
|
| Relação: |
http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/35655/pdf
http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/downloadSuppFile/35655/14384
|
| Formato: |
application/pdf
|
| Fonte: |
Acta Scientiarum. Biological Sciences; v. 41 (2019): Publicação contínua; e35655
Acta Scientiarum. Biological Sciences; v. 41 (2019): Publicação contínua; e35655
1807-863X
1679-9283
|
| Direitos: |
Direitos autorais 2019 Acta Scientiarum. Biological Sciences
http://creativecommons.org/licenses/by/4.0
|
