| Registro completo |
| Provedor de dados: |
Acta Amazonica
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| País: |
Brazil
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| Título: |
Transferability of heterologous primers in Brycon falcatus
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| Autores: |
PENHA,Diego dos Santos
SOUZA,Felipe Pinheiro de
LIMA,Ed Christian Suzuki de
URREA-ROJAS,Angela Maria
PANDOLFI,Victor César Freitas
YAMACHITA,Andrei Lincoln
POVH,Jayme Aparecido
LEITE,Natalia Gonçalves
PEREIRA,Ulisses de Pádua
LOPERA-BARRERO,Nelson Mauricio
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| Data: |
2020-09-01
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| Ano: |
2020
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| Palavras-chave: |
Bryconidae
Cross amplification
Genetic conservation
Genetic variability
SSR
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| Resumo: |
ABSTRACT The genusBryconcomprises fish species of significant socioeconomic and biological importance in Brazil. Despite that, the genetic knowledge about these species is scarce, especially regardingBrycon falcatus. Thus, the objective of this study was to evaluate the transferability of heterologous microsatellite primers inB. falcatus for the first time. Heterologous primers obtained from B. opalinus, B. hilarii, B. insignis, B. orbignyanus, B. amazonicus, Prochilodus argenteus, Prochilodus lineatus, Piaractus mesopotamicus, and Colossoma macropomum were evaluated. The primers that showed the best amplification patterns were applied to a sample of 22 individuals and the genetic parameters were calculated. Nine primers displayed satisfactory cross-amplification withB. falcatus: BoM5 (Brycon opalinus); Bh8, Bh13 and Bh16 (B. hilarii); Borg59 (B. orbignyanus); Bag22 (B. amazonicus); Par12 and Par80 (P. argenteus), and Cm1A8 (C. macropomum). The genetic parameters (number of alleles, effective alleles, allele richness, and expected and observed heterozygosity) and the polymorphic information content (PIC) confirmed the viability of these primers for population genetics analyses. Our study demonstrates the potential of transferability of microsatellite markers from related species and even different genera to B. falcatus, providing usefull tools for future population genetic studies in this species.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672020000300232
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| Editor: |
Instituto Nacional de Pesquisas da Amazônia
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| Relação: |
10.1590/1809-4392201904191
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| Formato: |
text/html
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| Fonte: |
Acta Amazonica v.50 n.3 2020
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| Direitos: |
info:eu-repo/semantics/openAccess
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