| Registro completo |
| Provedor de dados: |
Acta Amazonica
|
| País: |
Brazil
|
| Título: |
Protocol optimization for the detection of Trypanosoma cruzi DNA in açai (Euterpe oleraceae) pulp
|
| Autores: |
CARDOSO,Gabrielle Virgínia Ferreira
OLIVEIRA,Andrey Carlos do Sacramento de
SILVA,Andréia Silva da
SILVA,Marcos Clécio de Lemos
LIMA,Joelson Sousa
ROOS,Talita Bandeira
MORAES,Carina Martins de
|
| Data: |
2021-03-01
|
| Ano: |
2021
|
| Palavras-chave: |
Detection method
Foodborne disease
Food pathogen
Molecular detection
Chagas disease
|
| Resumo: |
ABSTRACT Chagas disease, caused by the protozoan Trypanosoma cruzi, has often been linked to oral transmission through açai consumption. Molecular methods that allow fast and accurate identification of the pathogen are important for the detection of the presence of the parasite in this food. This study aimed to optimize polymerase chain reaction (PCR)-based detection of T. cruzi DNA in açai pulp. Several dilutions of T. cruzi DTU TcI trypomastigote forms were cultured in liver infusion tryptose (LIT) medium. Trypanosoma cruzi DNA was extracted from the cells and subjected to PCR. Subsequently, culture dilutions were added to açai pulp to evaluate the detection threshold of the optimized PCR assay. We demonstrate that our assay can detect T. cruzi DNA in açai pulp at a concentration of 1.08 × 10-10 ng µL-1. We conclude that our optimized protocol is effective and can be used as an important tool for the detection of T. cruzi contamination in açaí.
|
| Tipo: |
Info:eu-repo/semantics/article
|
| Idioma: |
Inglês
|
| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672021000100079
|
| Editor: |
Instituto Nacional de Pesquisas da Amazônia
|
| Relação: |
10.1590/1809-4392201903426
|
| Formato: |
text/html
|
| Fonte: |
Acta Amazonica v.51 n.1 2021
|
| Direitos: |
info:eu-repo/semantics/openAccess
|
