| Registro completo |
| Provedor de dados: |
Acta Amazonica
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| País: |
Brazil
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| Título: |
LD5o of the bacteria Aeromonas hydrophila to matrinxã, Brycon amazonicus
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| Autores: |
Oliveira,Sarah Ragonha de
Souza,Rondon Tatsuta Yamane Baptista de
Brasil,Elenice Martins
Andrade,Jaqueline Inês Alves de
Nunes,Érica da Silva Santiago
Ono,Eduardo Akifumi
Affonso,Elizabeth Gusmão
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| Data: |
2011-01-01
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| Ano: |
2011
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| Palavras-chave: |
Aeromonas hydrophila
96-h LD50
Matrinxã
Tropical fishes
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| Resumo: |
In order to determine the lethal dose (96-h LD50) of the bacteria Aeromonas hydrophila to matrinxã, Brycon amazonicus, to be applied in challenge tests, 90 fish (63.23 ± 6.39 g) were divided into five treatments, with different bacterial solutions: T1 - Control (0.9% NaCl saline solution); T2 (4 x 10(11) cells/ mL); T3 (5 x 10(11) cells/ mL); T4 (1.36 x 10(12) cells/ mL) and T5 (3.06 x 10(12) cells/ mL). Fish were previously anesthetized with benzocaine (60 mg L-1), inoculated in the peritoneal cavity with the bacterial suspensions and then distributed into fifteen 80-L test chambers, where the water variables were monitored and fish mortality was observed. The experiment was randomly designed in three replicates and the 96-h LD50 was estimated according to the trimmed Spearman-Karber method. Water quality variables remained within adequate ranges for fish health and performance. Fish mortality rate increased with the bacterial concentrations of A. hydrophila (T1 = 0%; T2 = 16.66%; T3 = 44.44%; T4 = 72.22% and T5 = 100%), and the first mortalities were observed after 57 h, although the signs of the bacterial infection were already observed 24 h after the inoculation. The results indicate that the 96-h LD50 value of A. hydrophila to matrinxã is 6.66 x 10(11) cells/ mL.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672011000200019
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| Editor: |
Instituto Nacional de Pesquisas da Amazônia
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| Relação: |
10.1590/S0044-59672011000200019
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| Formato: |
text/html
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| Fonte: |
Acta Amazonica v.41 n.2 2011
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| Direitos: |
info:eu-repo/semantics/openAccess
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