| Registro completo |
| Provedor de dados: |
Anais da ABC (AABC)
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| País: |
Brazil
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| Título: |
High-fat diet and streptozotocin in the induction of type 2 diabetes mellitus: a new proposal
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| Autores: |
MAGALHÃES,DIEGO A. DE
KUME,WILLIAN T.
CORREIA,FRANCYELE S.
QUEIROZ,THAÍS S.
ALLEBRANDT NETO,EDGAR W.
SANTOS,MAÍSA P. DOS
KAWASHITA,NAIR H.
FRANÇA,SUÉLEM A. DE
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| Data: |
2019-01-01
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| Ano: |
2019
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| Palavras-chave: |
Diabetes mellitus type 2 model
High-fat diet
Streptozotocin
Rats
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| Resumo: |
Our objective was to establish a diabetes mellitus type 2 (DM2) model in rats using a high-fat diet and streptozotocin (HF-STZ). Male Wistar rats (240-250g) were divided into a control group (commercial feed), and HF-STZ group, (66.5%-commercial feed, 13.5%-lard, and 20%-sugar). STZ (40mg/kg i.p.) or vehicle was administered on the 13th day. An oral glucose tolerance test (OGTT) was performed (2.5mg of glucose/kg v.o.) on both groups. After 39 days of treatment, blood and tissue samples were collected for analyses. The weight gain after STZ administration was lower in the HF-STZ group than in the control group with reductions in muscle mass and adipose tissue. The HF-STZ group showed hyperglycemia after STZ administration (glucose on day 39: HF-STZ: 499 ± 60; control: 134 ± 9mg/dL). Serum glucagon was 23% lower, and insulin levels were unaltered. The HOMA index was 4-times higher in the HF-STZ. The HF-STZ group showed increased post-prandial (330%) and fasting (125%) triglycerides, and while glycogen content in the liver and muscles decreased (70-80%). The area under the curve (OGTT) was 282% higher in the HF-STZ group. The combination of high-fat diet with STZ (i.p) generated rats with hyperglycemia associated with hypertriglyceridemia and introduced many other alterations present in human DM2.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000100615
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| Editor: |
Academia Brasileira de Ciências
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| Relação: |
10.1590/0001-3765201920180314
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| Formato: |
text/html
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| Fonte: |
Anais da Academia Brasileira de Ciências v.91 n.1 2019
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| Direitos: |
info:eu-repo/semantics/openAccess
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