| Registro completo |
| Provedor de dados: |
Anais da ABC (AABC)
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| País: |
Brazil
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| Título: |
Effects of Jatropha curcas oil inLactuca sativa root tip bioassays
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| Autores: |
ANDRADE-VIEIRA,LARISSA F.
BOTELHO,CAROLINA M.
LAVIOLA,BRUNO G.
PALMIERI,MARCEL J.
PRAÇA-FONTES,MILENE M.
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| Data: |
2014-03-01
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| Ano: |
2014
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| Palavras-chave: |
Chromosomal aberration
Germination rate
Lactuca sativa
Phorbol esters
Root elongation
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| Resumo: |
Jatropha curcas L. (Euphorbiaceae) is important for biofuel production and as a feed ingredient for animal. However, the presence of phorbol esters in the oil and cake renders the seeds toxic. The toxicity ofJ. curcas oil is currently assessed by testing in animals, leading to their death. The identification of toxic and nontoxic improved varieties is important for the safe use of J. curcas seeds and byproducts to avoid their environmental toxicity. Hence, the aim of this study was to propose a short-term bioassay using a plant as a model to screen the toxicity of J. curcas oil without the need to sacrifice any animals. The toxicity of J. curcas oil was evident in germination, root elongation and chromosomal aberration tests in Lactuca sativa. It was demonstrated that J. curcas seeds contain natural compounds that exert phyto-, cyto- and genotoxic effects on lettuce, and that phorbol esters act as aneugenic agents, leading to the formation of sticky chromosomes and c-metaphase cells. In conclusion, the tests applied have shown reproducibility, which is important to verify the extent of detoxification and to determine toxic doses, thus reducing the numbers of animals that would be used for toxicity tests.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652014000100373
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| Editor: |
Academia Brasileira de Ciências
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| Relação: |
10.1590/0001-3765201420130041
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| Formato: |
text/html
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| Fonte: |
Anais da Academia Brasileira de Ciências v.86 n.1 2014
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| Direitos: |
info:eu-repo/semantics/openAccess
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