| Registro completo |
| Provedor de dados: |
Anais da ABC (AABC)
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| País: |
Brazil
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| Título: |
Cryopreservation of banana’s cv Grand Naine in vitro rhizomes
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| Autores: |
LONDE,LUCIANA C.N.
VENDRAME,WAGNER A.
SANAEI,MASSY
OLIVEIRA,ALEXANDRE B. DE
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| Data: |
2018-03-01
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| Ano: |
2018
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| Palavras-chave: |
Musa acumminata
In vitro conservation
Cryoprotectants
Pre-cooling
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| Resumo: |
ABSTRACT The preservation of banana genetic material is usually performed through seedlings. However, most banana cultivars do not produce seed and are propagated vegetatively. Therefore, cryopreservation is a feasible technique that allows the preservation of banana genotypes indefinitely. For the success of cryopreservation protocols, the selection of cryoprotectants and pre-freezing techniques are important factor. Therefore, the objective of this study was to verify the effects of different cryoprotectants with and without 1% phloroglucinol and pre-cooling periods on the development of a protocol for cryopreservation of in vitro rhizomes ofMusa accuminata(AAA) cv Grand Naine banana. The addition of 1% phloroglucinol to the cryoprotective solutions, such as PVS2 enhanced recovery of cryopreserved banana rhizomes. In addition, pre-cooling of explants in ice for 3 hours in PVS2 + 1% of phloroglucinol allowed efficient cryopreservation of banana rhizomes, followed by successful recovery and regeneration of in vitro shoots of banana cv Grand Naine.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000100495
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| Editor: |
Academia Brasileira de Ciências
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| Relação: |
10.1590/0001-3765201720170056
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| Formato: |
text/html
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| Fonte: |
Anais da Academia Brasileira de Ciências v.90 n.1 2018
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| Direitos: |
info:eu-repo/semantics/openAccess
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